CP24222Integration of seed priming with nano-sized chitosan-proline and biochar application improves salt tolerance in differentially responding genotypes of alfalfa (Medicago sativa)
Integrated use of nano-sized chitosan-proline (NsCP) seed priming and biochar significantly improved the salt tolerance in alfalfa (Medicago sativa) genotypes. The combined application of NsCP and biochar was more effective in enhancing salt tolerance than individual treatments alone. NsCP seed priming enhanced the salinity stress tolerance in alfalfa genotypes by facilitating osmotic adjustment (proline accumulation), maintaining ionic homeostasis (higher K+/Na+ ratio), and increasing the levels of α-tocopherol, flavonoids, and the activities of antioxidant enzymes.