Mechanical mastication and prescribed fire in conifer–hardwood chaparral: differing responses of ectomycorrhizae and truffles
Darlene Southworth A C , Jessica Donohue A , Jonathan L. Frank A and Jennifer Gibson B
+ Author Affiliations
- Author Affiliations
A Department of Biology, Southern Oregon University, Ashland, OR 97520-5071, USA.
B Whiskeytown National Recreation Area, PO Box 188, Whiskeytown, CA 96095, USA.
C Corresponding author. Email: southworth@sou.edu
International Journal of Wildland Fire 20(7) 888-896 https://doi.org/10.1071/WF10033
Submitted: 9 March 2010 Accepted: 15 February 2011 Published: 1 September 2011
Abstract
Fire-prone hardwood–conifer chaparral comprises a significant component of vegetation in seasonally dry areas where prescribed burns of standing vegetation are limited by air-quality restrictions and narrow climatic opportunities for burning. Mechanical mastication is used by land managers to reduce aerial fuels. When burned, the dry masticated slash layer may result in prolonged soil heating, particularly of the upper soil layers, which contain ectomycorrhizal roots and seasonal truffles (hypogeous fungal sporocarps). The purpose of this study was to examine the effects of mechanical mastication followed by prescribed fire on ectomycorrhizae and truffles. We treated blocks with mechanical mastication only, mechanical mastication followed by prescribed fire, prescribed fire only, and no treatment. Five years after the prescribed burn, soils with ectomycorrhizal roots were sampled at the canopy dripline of Pinus attenuata and Quercus kelloggii and surveyed for truffles. Ectomycorrhizae and truffles were described by morphology and by DNA sequences of the internal transcribed spacer region. Ectomycorrhizal communities did not differ among treatments. However, burning reduced the abundance and species richness of truffles in both controls and masticated vegetation. We conclude that prescribed burning of mechanically masticated slash does not harm ectomycorrhizal communities, but does inhibit fruiting of truffles.
Additional keywords: brush mastication, fuels reduction, hypogeous fungi, oak–chaparral.
References
Agerer R (1991) Characterization of ectomycorrhiza. Methods in Microbiology 23, 25–73.| Characterization of ectomycorrhiza.Crossref | GoogleScholarGoogle Scholar |
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. Journal of Molecular Biology 215, 403–410.
Amaranthus MP, Trappe JM (1993) Effects of soil erosion and ecto- and VA-mycorrhizal inoculum potential following wildfire in southwestern Oregon. Plant and Soil 150, 41–49.
| Effects of soil erosion and ecto- and VA-mycorrhizal inoculum potential following wildfire in southwestern Oregon.Crossref | GoogleScholarGoogle Scholar |
Baar J, Horton TR, Kretzer AM, Bruns TD (1999) Mycorrhizal colonization of Pinus muricata from resistant propagules after a stand-replacing wildfire. New Phytologist 143, 409–418.
| Mycorrhizal colonization of Pinus muricata from resistant propagules after a stand-replacing wildfire.Crossref | GoogleScholarGoogle Scholar |
Bastias BA, Xu Z, Cairney JWG (2006) Influence of long term repeated prescribed burning on mycelial communities of ectomycorrhizal fungi. New Phytologist 172, 149–158.
| Influence of long term repeated prescribed burning on mycelial communities of ectomycorrhizal fungi.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhtVyisrbL&md5=37747a17f2e91075e1e01db72ca6cdabCAS |
Boerner RE, Huang JJ, Hart SC (2009) Impacts of fire and fire surrogate treatments on forest soil properties: a meta-analytical approach. Ecological Applications 19, 338–358.
| Impacts of fire and fire surrogate treatments on forest soil properties: a meta-analytical approach.Crossref | GoogleScholarGoogle Scholar |
Bradley T, Gibson J, Bunn W (2006) Fuels management and non-native plant species: an evaluation for fire and fire surrogate treatments in a chaparral plant community. (Joint Fire Science Program) Available at http://jfsp.nifc.gov/projects/01B-3-3-27/01B-3-3-27_final_report.pdf [Verified March 2010]
Busse MD, Hubbert KR, Fiddler GO, Shestak CJ, Powers RF (2005) Lethal soil temperatures during burning of masticated forest residues. International Journal of Wildland Fire 14, 267–276.
| Lethal soil temperatures during burning of masticated forest residues.Crossref | GoogleScholarGoogle Scholar |
Cairney JWG, Bastias BA (2007) Influences of fire on forest soil fungal communities. Canadian Journal of Forest Research 37, 207–215.
| Influences of fire on forest soil fungal communities.Crossref | GoogleScholarGoogle Scholar |
Cairney JWG, Chambers SM (Eds) (1999) ‘Ectomycorrhizal Fungi: Key Genera in Profile.’ (Springer: New York)
Castellano MA, Trappe JM, Maser Z, Maser C (1989) ‘Key to Spores of the Genera of Hypogeous Fungi of North Temperate Forests.’ (Mad River Press: Eureka, CA)
Castellano MA, Trappe JM, Luoma DL (2004) Sequestrate Fungi. In ‘Biodiversity of Fungi’. (Eds GM Mueller, GF Bills, MS Foster) pp. 197–213. (Elsevier-Academic Press: San Francisco, CA)
Claridge AW, Trappe JM, Hansen K (2009) Do fungi have a role as soil stabilizers and remediators after forest fire? Forest Ecology and Management 257, 1063–1069.
| Do fungi have a role as soil stabilizers and remediators after forest fire?Crossref | GoogleScholarGoogle Scholar |
de Román M, de Miguel AM (2005) Post-fire seasonal and annual dynamics of the ectomycorrhizal community in a Quercus ilex L. forest over a 3-year period. Mycorrhiza 15, 471–482.
| Post-fire seasonal and annual dynamics of the ectomycorrhizal community in a Quercus ilex L. forest over a 3-year period.Crossref | GoogleScholarGoogle Scholar |
Fogel R, Trappe JM (1978) Fungus consumption (mycophagy) by small animals. Northwest Science 52, 1–31.
Frank JL, Barry S, Southworth D (2006) Mammal mycophagy and dispersal of mycorrhizal inoculum in Oregon white oak woodlands. Northwest Science 80, 264–273.
Frank JL, Anglin S, Carrington EM, Taylor DS, Viratos B, Southworth D (2009) Rodent dispersal of fungal spores promotes seedling establishment away from mycorrhizal networks in Quercus garryana. Botany 87, 821–829.
| Rodent dispersal of fungal spores promotes seedling establishment away from mycorrhizal networks in Quercus garryana.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhtF2kurrF&md5=b0bf456c4053342c9d112d3ae417d2f9CAS |
Fry DL, Stephens SL (2006) Influence of humans and climate on the fire history of a ponderosa pine–mixed conifer forest in the south-eastern Klamath Mountains, California. Forest Ecology and Management 223, 428–438.
| Influence of humans and climate on the fire history of a ponderosa pine–mixed conifer forest in the south-eastern Klamath Mountains, California.Crossref | GoogleScholarGoogle Scholar |
Fujimura KE, Smith JE, Horton TR, Weber NS, Spatafora JW (2005) Pezizalean mycorrhizas and sporocarps in ponderosa pine (Pinus ponderosa) after prescribed fires in eastern Oregon, USA. Mycorrhiza 15, 79–86.
| Pezizalean mycorrhizas and sporocarps in ponderosa pine (Pinus ponderosa) after prescribed fires in eastern Oregon, USA.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD2M7isFequw%3D%3D&md5=7593ba2b76a6914c1b5bcb24a5111fe3CAS |
Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes – application to the identification of mycorrhizae and rusts. Molecular Ecology 2, 113–118.
| ITS primers with enhanced specificity for basidiomycetes – application to the identification of mycorrhizae and rusts.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXlslOmsro%3D&md5=a1cb511bde92526ff830a6e8a8796c3bCAS |
Glitzenstein JS, Streng DR, Achtemeier GL, Naeher LP, Wade DD (2006) Fuels and fire behavior in chipped and unchipped blocks: implications for land management near the wildland–urban interface. Forest Ecology and Management 236, 18–29.
| Fuels and fire behavior in chipped and unchipped blocks: implications for land management near the wildland–urban interface.Crossref | GoogleScholarGoogle Scholar |
Goodman DM, Durall DM, Trofymow JA, Berch SM (Eds) (2002) ‘Concise Descriptions of North American Ectomycorrhizas.’ (Mycologue Publications: Victoria, BC)
Hagerman SM, Jones MD, Bradfield GE, Gillespie M, Durall DM (1999) Effects of clear-cut logging on the diversity and persistence of ectomycorrhizae at a subalpine forest. Canadian Journal of Forest Research 29, 124–134.
| Effects of clear-cut logging on the diversity and persistence of ectomycorrhizae at a subalpine forest.Crossref | GoogleScholarGoogle Scholar |
Hart SC, DeLuca TH, Newman GS, MacKenzie MD, Boyle SI (2005) Post-fire vegetative dynamics as drivers of microbial community structure and function in forest soils. Forest Ecology and Management 220, 166–184.
| Post-fire vegetative dynamics as drivers of microbial community structure and function in forest soils.Crossref | GoogleScholarGoogle Scholar |
Johnson CN (1996) Interactions between mammals and ectomycorrhizal fungi. Trends in Ecology & Evolution 11, 503–507.
| Interactions between mammals and ectomycorrhizal fungi.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3M7itFGlsA%3D%3D&md5=8f9ee5d728528c5caf0db8ac0d346f47CAS |
Jonsson L, Dahlberg A, Nilsson MC, Zackrisson O, Kåren O (1999) Ectomycorrhizal fungal communities in late-successional Swedish boreal forests, and their composition following wildfire. Molecular Ecology 8, 205–215.
| Ectomycorrhizal fungal communities in late-successional Swedish boreal forests, and their composition following wildfire.Crossref | GoogleScholarGoogle Scholar |
Kane JM, Knapp EE, Varner JM (2006) Variability in loading of mechanically masticated fuel beds in northern California and south-western Oregon. USDA Forest Service, Rocky Mountain Research Station RMRS-P-41. (Fort Collins, CO)
Kane JM, Varner JM, Knapp EE (2009) Novel fuelbed characteristics associated with mechanical mastication treatments in northern California and south-western Oregon. International Journal of Wildland Fire 18, 686–697.
| Novel fuelbed characteristics associated with mechanical mastication treatments in northern California and south-western Oregon.Crossref | GoogleScholarGoogle Scholar |
Keeley JE (2009) Fire intensity, fire severity and burn severity: a brief review and suggested usage. International Journal of Wildland Fire 18, 116–126.
| Fire intensity, fire severity and burn severity: a brief review and suggested usage.Crossref | GoogleScholarGoogle Scholar |
Kennedy PG, Izzo AD, Bruns TD (2003) There is high potential for the formation of common mycorrhizal networks between understorey and canopy trees in a mixed evergreen forest. Journal of Ecology 91, 1071–1080.
| There is high potential for the formation of common mycorrhizal networks between understorey and canopy trees in a mixed evergreen forest.Crossref | GoogleScholarGoogle Scholar |
Kozlowski TT, Kramer PJ, Pallardy SG (1991) ‘The Physiological Ecology of Woody Plants.’ (Academic Press: San Diego, CA)
Luoma D, Frenkel RE, Trappe JM (1991) Fruiting of hypogeous fungi in Oregon Douglas-fir forests. Mycologia 83, 335–353.
| Fruiting of hypogeous fungi in Oregon Douglas-fir forests.Crossref | GoogleScholarGoogle Scholar |
Mallory JI, Colwell WL, Powell WR (1973) Soils and vegetation of the French Gulch quadrangle (24D–1,2,3,4), Trinity and Shasta counties, California. USDA Forest Service, Pacific Southwest Forest and Range Experiment Station, Resource Bulletin PSW-12. (Berkeley, CA)
McCarthy C (1998) Chromas 1.45. (Griffith University: Southport, QLD)
McCune B, Grace JB (2002) ‘Analysis of Ecological Communities.’ (MjM Software: Gleneden Beach, OR)
McCune B, Mefford MJ (1999) ‘PC-ORD, Multivariate Analysis of Ecological Data, Version 4.’ (MjM Software: Gleneden Beach, OR)
Meyer MC, North MP, Kelt DA (2005) Short-term effects of fire and forest thinning on truffle abundance and consumption by Neotamias speciosus in the Sierra Nevada of California. Canadian Journal of Forest Research 35, 1061–1070.
| Short-term effects of fire and forest thinning on truffle abundance and consumption by Neotamias speciosus in the Sierra Nevada of California.Crossref | GoogleScholarGoogle Scholar |
Meyer MC, North MP, Roberts SL (2008) Truffle abundance in recently prescribed burned and unburned forests in Yosemite National Park: implications for mycophagous mammals. Fire Ecology 4, 105–114.
| Truffle abundance in recently prescribed burned and unburned forests in Yosemite National Park: implications for mycophagous mammals.Crossref | GoogleScholarGoogle Scholar |
Miller SL, McClean TM, Stanton NL, Williams SE (1998) Mycorrhization, physiognomy, and first-year survivability of conifer seedlings following natural fire in Grand Teton National Park. Canadian Journal of Forest Research 28, 115–122.
| Mycorrhization, physiognomy, and first-year survivability of conifer seedlings following natural fire in Grand Teton National Park.Crossref | GoogleScholarGoogle Scholar |
Morris MH, Smith ME, Rizzo DM, Rejmánek M, Bledsoe CS (2008) Contrasting ectomycorrhizal fungal communities on the roots of co-occurring oaks (Quercus spp.) in a California woodland. New Phytologist 178, 167–176.
| Contrasting ectomycorrhizal fungal communities on the roots of co-occurring oaks (Quercus spp.) in a California woodland.Crossref | GoogleScholarGoogle Scholar |
Moser AM, Petersen CA, D’Allura JA, Southworth D (2005) Comparison of ectomycorrhizas of Quercus garryana (Fagaceae) on serpentine and non-serpentine soils in south-western Oregon. American Journal of Botany 92, 224–230.
| Comparison of ectomycorrhizas of Quercus garryana (Fagaceae) on serpentine and non-serpentine soils in south-western Oregon.Crossref | GoogleScholarGoogle Scholar |
Moser AM, Frank JL, D’Allura JA, Southworth D (2009) Mycorrhizal communities of Quercus garryana on serpentine soils exhibit edaphic tolerance. Plant and Soil 315, 185–194.
| Mycorrhizal communities of Quercus garryana on serpentine soils exhibit edaphic tolerance.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXjvFeltQ%3D%3D&md5=1572d0ab015a14782c63744dbca6d21dCAS |
Neary DG, Klopatek CC, DeBano LF, Ffolliott PF (1999) Fire effects on belowground sustainability: a review and synthesis. Forest Ecology and Management 122, 51–71.
| Fire effects on belowground sustainability: a review and synthesis.Crossref | GoogleScholarGoogle Scholar |
North M, Trappe JM, Franklin J (1997) Standing crop and animal consumption of fungal sporocarps in Pacific Northwest forests. Ecology 78, 1543–1554.
| Standing crop and animal consumption of fungal sporocarps in Pacific Northwest forests.Crossref | GoogleScholarGoogle Scholar |
Noss RF, Franklin JF, Baker WL, Schornnagel T, Moyle PB (2006) Managing fire-prone forests in the western United States. Frontiers in Ecology and the Environment 4, 481–487.
| Managing fire-prone forests in the western United States.Crossref | GoogleScholarGoogle Scholar |
Odion DC, Davis FW (2000) Fire, soil heating, and the formation of vegetation patterns in chaparral. Ecological Monographs 70, 149–169.
| Fire, soil heating, and the formation of vegetation patterns in chaparral.Crossref | GoogleScholarGoogle Scholar |
Peay KG, Garbelotto M, Bruns TD (2009) Spore heat resistance plays an important role in disturbance-mediated assemblage shift of ectomycorrhizal fungi colonizing Pinus muricata seedlings. Journal of Ecology 97, 537–547.
| Spore heat resistance plays an important role in disturbance-mediated assemblage shift of ectomycorrhizal fungi colonizing Pinus muricata seedlings.Crossref | GoogleScholarGoogle Scholar |
Pilz DP, Perry DA (1984) Impact of clearcutting and slash burning on ectomycorrhizal associations of Douglas-fir seedlings. Canadian Journal of Forest Research 14, 94–100.
| Impact of clearcutting and slash burning on ectomycorrhizal associations of Douglas-fir seedlings.Crossref | GoogleScholarGoogle Scholar |
Poff RJ (1996) Effects of silvicultural practices and wildfire on productivity of forest soils. Sierra Nevada ecosystem project: final report to Congress, vol. II, assessments and scientific basis for management options. (University of California: Davis, CA) Available at http://ceres.ca.gov/snep/pubs/v2s2.html [Verified March 2010]
Reiner AL, Vaillant NM, Fites-Kaufman J, Dailey SM (2009) Mastication and prescribed fire impacts on fuels in a 25-year old ponderosa pine plantation, southern Sierra Nevada. Forest Ecology and Management 258, 2365–2372.
| Mastication and prescribed fire impacts on fuels in a 25-year old ponderosa pine plantation, southern Sierra Nevada.Crossref | GoogleScholarGoogle Scholar |
Schwilk DW, Keeley JE, Knapp EE, McIver J, Bailey JD, Fettig CJ, Fiedler CE, Harrod RY, Moghaddas JJ, Outcalt KW, Skinner CN, Stephens SL, Waldrop TA, Yaussy DA, Youngblood A (2009) The national fire and fire surrogate study: effects of fuel reduction methods on forest vegetation structure and fuels. Ecological Applications 19, 285–304.
| The national fire and fire surrogate study: effects of fuel reduction methods on forest vegetation structure and fuels.Crossref | GoogleScholarGoogle Scholar |
Simard SW (2009) The foundational role of mycorrhizal networks in self-organization of interior Douglas-fir forests. Forest Ecology and Management 258, S95–S107.
| The foundational role of mycorrhizal networks in self-organization of interior Douglas-fir forests.Crossref | GoogleScholarGoogle Scholar |
Smith SE, Read DJ (2008) ‘Mycorrhizal Symbiosis.’ 3rd edn (Academic Press: San Diego, CA)
Smith JE, McKay D, Niwa CG, Thies WG, Brenner G, Spatafora JW (2004) Short-term effects of seasonal prescribed burning on the ectomycorrhizal fungal community and fine root biomass in ponderosa pine stands in the Blue Mountains of Oregon. Canadian Journal of Forest Research 34, 2477–2491.
| Short-term effects of seasonal prescribed burning on the ectomycorrhizal fungal community and fine root biomass in ponderosa pine stands in the Blue Mountains of Oregon.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXisVKgtr4%3D&md5=62e51f588deb8fef77c3bff667aa5164CAS |
Smith JE, McKay D, Brenner G, McIver J, Spatafora JW (2005) Early impacts of forest restoration treatments on the ectomycorrhizal fungal community and fine root biomass in a mixed conifer forest. Journal of Applied Ecology 42, 526–535.
| Early impacts of forest restoration treatments on the ectomycorrhizal fungal community and fine root biomass in a mixed conifer forest.Crossref | GoogleScholarGoogle Scholar |
Smith ME, Douhan GW, Rizzo DM (2007) Ectomycorrhizal community structure in a xeric Quercus woodland based on rDNA sequence analysis of sporocarps and pooled roots. New Phytologist 174, 847–863.
| Ectomycorrhizal community structure in a xeric Quercus woodland based on rDNA sequence analysis of sporocarps and pooled roots.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXnt1amtb0%3D&md5=17d01dbd4bd0b5f40f0a6e95eb909a8fCAS |
Smith ME, Douhan GW, Fremier AK, Rizzo DM (2009) Are true multihost fungi the exception or the rule? Dominant ectomycorrhizal fungi on Pinus sabiniana differ from those on co-occurring Quercus species. New Phytologist 182, 295–299.
| Are true multihost fungi the exception or the rule? Dominant ectomycorrhizal fungi on Pinus sabiniana differ from those on co-occurring Quercus species.Crossref | GoogleScholarGoogle Scholar |
Southworth D, Valentine LL, He XH, Swenson W, Bledsoe CS, Horwath WR (2005) Application of network theory to mycorrhizal networks. Mycorrhiza 15, 589–595.
| Application of network theory to mycorrhizal networks.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD2MngtlGhuw%3D%3D&md5=1af876efb1a0be64bdf100e91e78ae84CAS |
Stendell ER, Horton TR, Bruns TD (1999) Early effects of prescribed fire on the structure of the ectomycorrhizal fungus community in a Sierra Nevada ponderosa pine forest. Mycological Research 103, 1353–1359.
| Early effects of prescribed fire on the structure of the ectomycorrhizal fungus community in a Sierra Nevada ponderosa pine forest.Crossref | GoogleScholarGoogle Scholar |
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25, 4876–4882.
| The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXntFyntQ%3D%3D&md5=551fcd59dadec8122db4ffda0e902f70CAS |
Trappe JM (1979) The orders, families, and genera of hypogeous Ascomycotina (truffles and their relatives). Mycotaxon 9, 297–340.
Trappe JM, Nicholls AO, Claridge AW, Cork SJ (2006) Prescribed burning in a Eucalyptus woodland suppresses fruiting of hypogeous fungi, an important food source for mammals. Mycological Research 110, 1333–1339.
| Prescribed burning in a Eucalyptus woodland suppresses fruiting of hypogeous fungi, an important food source for mammals.Crossref | GoogleScholarGoogle Scholar |
Trappe M, Evans F, Trappe JM (2007) ‘Field Guide to North American truffles.’ (Ten Speed Press: Berkeley, CA)
USDA Forest Service (2001) National fire plan. (USDA Forest Service: Washington, DC) Available at http://www.forestsandrangelands.gov/overview/index.shtml [Verified March 2010]
Valentine LL, Fiedler TL, Hart AN, Petersen CA, Berninghausen HK, Southworth D (2004) Biodiversity of ectomycorrhizal fungi associated with Quercus garryana. Canadian Journal of Botany 82, 123–135.
| Biodiversity of ectomycorrhizal fungi associated with Quercus garryana.Crossref | GoogleScholarGoogle Scholar |
Visser S (1995) Ectomycorrhizal fungal succession in jack pine stands following wildfire. New Phytologist 129, 389–401.
| Ectomycorrhizal fungal succession in jack pine stands following wildfire.Crossref | GoogleScholarGoogle Scholar |
White TJ, Bruns TD, Lee SB, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In ‘PCR Protocols – A Guide to Methods and Applications’. (Eds MA Innis, DH Gelfand, JJ Sninsky, TJ White) pp. 315–322. (Academic Press: New York)
