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RESEARCH ARTICLE

Community-acquired Clostridium difficile infection and Australian food animals

Michele M Squire A , Daniel R Knight A and Thomas V Riley A B
+ Author Affiliations
- Author Affiliations

A Microbiology and Immunology
School of Pathology and Laboratory Medicine
The University of Western Australia
Queen Elizabeth II Medical Centre
Nedlands, WA 6009, Australia

B Tel: +61 8 6383 4355, Fax: +61 8 9346 2912, Email: thomas.riley@uwa.edu.au

Microbiology Australia 36(3) 111-113 https://doi.org/10.1071/MA15040
Published: 6 August 2015

Abstract

Clostridium difficile is an anaerobic Gram positive spore-forming bacterium, the leading cause of infectious diarrhoea (C. difficile infection; CDI) in hospitalised humans. The assumption that CDI is primarily a hospital-acquired infection is being questioned. Community-acquired CDI (CA-CDI) is increasing1 particularly in groups previously considered at low risk2,3. In Australia, CA-CDI rates doubled during 2011 and increased by 24% between 2011 and 20124. Two potentially high-risk practices in Australian food animal husbandry may present a risk for CA-CDI: slaughtering of neonatal animals for food, and effluent recycling to agriculture.


References

[1]  Khanna, S. et al. (2012) The epidemiology of community-acquired Clostridium difficile infection: a population-based study. Am. J. Gastroenterol. 107, 89–95.
The epidemiology of community-acquired Clostridium difficile infection: a population-based study.Crossref | GoogleScholarGoogle Scholar | 22108454PubMed |

[2]  Naggie, S. et al. (2010) Community-associated Clostridium difficile infection: experience of a veteran affairs medical center in southeastern USA. Infection 38, 297–300.
Community-associated Clostridium difficile infection: experience of a veteran affairs medical center in southeastern USA.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3cjgtVKmtA%3D%3D&md5=f470efde40972d33f8ce32bb51edef92CAS | 20454827PubMed |

[3]  Wilcox, M.H. et al. (2008) A case-control study of community-associated Clostridium difficile infection. J. Antimicrob. Chemother. 62, 388–396.
A case-control study of community-associated Clostridium difficile infection.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXosVOgtr4%3D&md5=e5d9cb96e5989de0ede2ed0b5d0c411eCAS | 18434341PubMed |

[4]  Slimings, C. et al. (2014) Increasing incidence of Clostridium difficile infection, Australia, 2011-2012. Med. J. Aust. 200, 272–276.
Increasing incidence of Clostridium difficile infection, Australia, 2011-2012.Crossref | GoogleScholarGoogle Scholar | 24641152PubMed |

[5]  Bauer, M.P. et al. (2009) Clinical and microbiological characteristics of community-onset Clostridium difficile infection in The Netherlands. Clin. Microbiol. Infect. 15, 1087–1092.
Clinical and microbiological characteristics of community-onset Clostridium difficile infection in The Netherlands.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD1MjntFyntA%3D%3D&md5=ec092093d1001fc8cc9704cffbd51e34CAS | 19624512PubMed |

[6]  Eyre, D.W. et al. (2013) Diverse sources of C. difficile infection identified on whole-genome sequencing. N. Engl. J. Med. 369, 1195–1205.
Diverse sources of C. difficile infection identified on whole-genome sequencing.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhsFGktLjI&md5=c1805bba409bc96fc5b985e3018e58ecCAS | 24066741PubMed |

[7]  Foster, N.F. et al. (2014) Epidemiology of Clostridium difficile infection in two tertiary-care hospitals in Perth, Western Australia: a cross-sectional study. New Microbes New Infect. 2, 64–71.
Epidemiology of Clostridium difficile infection in two tertiary-care hospitals in Perth, Western Australia: a cross-sectional study.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC2M3ksVGisg%3D%3D&md5=f2dd25531767384fd0236e889c38294fCAS | 25356346PubMed |

[8]  Huber, C.A. et al. (2014) Surveillance snapshot of Clostridium difficile infection in hospitals across Queensland detects binary toxin producing ribotype UK 244. Commun. Dis. Intell. Q. Rep. 38, E279–E284.
| 25631588PubMed |

[9]  Knetsch, C.W. et al. (2014) Whole genome sequencing reveals potential spread of Clostridium difficile between humans and farm animals in the Netherlands, 2002 to 2011. Euro Surveill. 19, 20954.
| 1:STN:280:DC%2BC2M3pslehuw%3D%3D&md5=6076668097e5930bcb05311832a1b95cCAS | 25411691PubMed |

[10]  Songer, J.G. et al. (2009) Clostridium difficile in retail meat products, USA, 2007. Emerg. Infect. Dis. 15, 819–821.
Clostridium difficile in retail meat products, USA, 2007.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXlslOgsrg%3D&md5=4224a30f300164f211f5ce5be48cf3f9CAS | 19402980PubMed |

[11]  Bauer, M.P. et al. (2011) Clostridium difficile infection in Europe: a hospital-based survey. Lancet 377, 63–73.
Clostridium difficile infection in Europe: a hospital-based survey.Crossref | GoogleScholarGoogle Scholar | 21084111PubMed |

[12]  Jhung, M.A. et al. (2008) Toxinotype V Clostridium difficile in humans and food animals. Emerg. Infect. Dis. 14, 1039–1045.
Toxinotype V Clostridium difficile in humans and food animals.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXptFyis74%3D&md5=968b71d1d35628559d58ca9f6cf1115aCAS | 18598622PubMed |

[13]  Keel, M.K. et al. (2006) The comparative pathology of Clostridium difficile-associated disease. Vet. Pathol. 43, 225–240.
The comparative pathology of Clostridium difficile-associated disease.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xlt1alu70%3D&md5=06d6697e04e17cca6908b84f6b6dacb2CAS | 16672570PubMed |

[14]  Rupnik, M. et al. (2010) Clostridium difficile: its potential as a source of foodborne disease. Adv. Food Nutr. Res. 60C, 53–66.
Clostridium difficile: its potential as a source of foodborne disease.Crossref | GoogleScholarGoogle Scholar |

[15]  Rodriguez-Palacios, A. et al. (2013) Clostridium difficile in foods and animals: history and measures to reduce exposure. Anim. Health Res. Rev. 14, 11–29.
Clostridium difficile in foods and animals: history and measures to reduce exposure.Crossref | GoogleScholarGoogle Scholar | 23324529PubMed |

[16]  Weese, J.S. et al. (2010) Longitudinal investigation of Clostridium difficile shedding in piglets. Anaerobe 16, 501–504.
Longitudinal investigation of Clostridium difficile shedding in piglets.Crossref | GoogleScholarGoogle Scholar | 20708700PubMed |

[17]  Bouttier, S. et al. (2010) Clostridium difficile in ground meat France. Emerg. Infect. Dis. 16, 733–735.
Clostridium difficile in ground meat France.Crossref | GoogleScholarGoogle Scholar | 20350408PubMed |

[18]  Indra, A. et al. (2009) Clostridium difficile: a new zoonotic agent? Wien. Klin. Wochenschr. 121, 91–95.
Clostridium difficile: a new zoonotic agent?Crossref | GoogleScholarGoogle Scholar | 19280132PubMed |

[19]  Von Abercron, S.M. et al. (2009) Low occurrence of Clostridium difficile in retail ground meat in Sweden. J. Food Prot. 72, 1732–1734.
| 19722410PubMed |

[20]  Jöbstl, M. et al. (2010) Clostridium difficile in raw products of animal origin. Int. J. Food Microbiol. 138, 172–175.
Clostridium difficile in raw products of animal origin.Crossref | GoogleScholarGoogle Scholar | 20079946PubMed |

[21]  Bouttier, S. et al. (2007) Screening for Clostridium difficile in meat from French retailers. In European Congress of Clinical Microbiology and Infectious Diseases, Munchen.

[22]  Rodriguez-Palacios, A. et al. (2007) Clostridium difficile in retail ground meat, Canada. Emerg. Infect. Dis. 13, 485–487.
Clostridium difficile in retail ground meat, Canada.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXjvVOktro%3D&md5=da7f670077895d29862bf7f3dc645a54CAS | 17552108PubMed |

[23]  Weese, J.S. et al. (2009) Detection and enumeration of Clostridium difficile spores in retail beef and pork. Appl. Environ. Microbiol. 75, 5009–5011.
Detection and enumeration of Clostridium difficile spores in retail beef and pork.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhtVSgtrvO&md5=b2292fabb237537f58ddaeaa304b0bc6CAS | 19525267PubMed |

[24]  Rodriguez-Palacios, A. et al. (2011) Moist-heat resistance, spore aging, and superdormancy in Clostridium difficile. Appl. Environ. Microbiol. 77, 3085–3091.
Moist-heat resistance, spore aging, and superdormancy in Clostridium difficile.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhtVeisr%2FP&md5=04e0798f149b58b8aa8a7a649153d259CAS | 21398481PubMed |

[25]  Bakri, M.M. et al. (2009) Clostridium difficile in ready-to-eat salads, Scotland. Emerg. Infect. Dis. 15, 817–818.
Clostridium difficile in ready-to-eat salads, Scotland.Crossref | GoogleScholarGoogle Scholar | 19402979PubMed |

[26]  Metcalf, D. et al. (2011) Clostridium difficile in seafood and fish. Anaerobe 17, 85–86.
Clostridium difficile in seafood and fish.Crossref | GoogleScholarGoogle Scholar | 21376822PubMed |

[27]  Metcalf, D.S. et al. (2010) Clostridium difficile in vegetables, Canada. Lett. Appl. Microbiol. 51, 600–602.
Clostridium difficile in vegetables, Canada.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3cbmtlWnsw%3D%3D&md5=bd86665ca9b0038ed2c8316293a4a4b2CAS | 21069911PubMed |

[28]  Squire, M.M. et al. (2011) Detection of Clostridium difficile after treatment in a two-stage pond system. In Manipulating Pig Production XIII (van Barneveld, R.J., ed), p. 215, APSA Biennial Conference, Australasian Pig Science Association.

[29]  Knight, D.R. et al. (2015) Nationwide surveillance study of Clostridium difficile in Australian neonatal pigs shows high prevalence and heterogeneity of PCR ribotypes. Appl. Environ. Microbiol. 81, 119–123.
Nationwide surveillance study of Clostridium difficile in Australian neonatal pigs shows high prevalence and heterogeneity of PCR ribotypes.Crossref | GoogleScholarGoogle Scholar | 25326297PubMed |

[30]  Koene, M.G. et al. (2012) Clostridium difficile in Dutch animals: their presence, characteristics and similarities with human isolates. Clin. Microbiol. Infect. 18, 778–784.
Clostridium difficile in Dutch animals: their presence, characteristics and similarities with human isolates.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC38zlvVSmsQ%3D%3D&md5=e36fe33ef8f35bb62ff290d3075dc54fCAS | 21919997PubMed |

[31]  Chan, G. et al. (2013) A retrospective study on the etiological diagnoses of diarrhea in neonatal piglets in Ontario, Canada, between 2001 and 2010. Can. J. Vet. Res. 77, 254–260.
| 24124267PubMed |

[32]  Susick, E.K. et al. (2012) Longitudinal study comparing the dynamics of Clostridium difficile in conventional and antimicrobial free pigs at farm and slaughter. Vet. Microbiol. 157, 172–178.
Longitudinal study comparing the dynamics of Clostridium difficile in conventional and antimicrobial free pigs at farm and slaughter.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC38vgtlGksg%3D%3D&md5=cf9a614b2b5de89bb6fdb5d491255edaCAS | 22243897PubMed |

[33]  Knight, D.R. et al. (2013) Cross-sectional study reveals high prevalence of Clostridium difficile non-PCR ribotype 078 strains in Australian veal calves at slaughter. Appl. Environ. Microbiol. 79, 2630–2635.
Cross-sectional study reveals high prevalence of Clostridium difficile non-PCR ribotype 078 strains in Australian veal calves at slaughter.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXlvVKlsb8%3D&md5=fe41bf93ea1f80d722914da6423b5d7aCAS | 23396338PubMed |

[34]  Rodriguez-Palacios, A. et al. (2006) Clostridium difficile PCR ribotypes in calves, Canada. Emerg. Infect. Dis. 12, 1730–1736.
Clostridium difficile PCR ribotypes in calves, Canada.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xht1entbzP&md5=948270fd31304c62ee2f9aeb853c549aCAS | 17283624PubMed |

[35]  Houser, B.A. et al. (2012) Prevalence of Clostridium difficile toxin genes in the feces of veal calves and incidence of ground veal contamination. Foodborne Pathog. Dis. 9, 32–36.
Prevalence of Clostridium difficile toxin genes in the feces of veal calves and incidence of ground veal contamination.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xjs1Wisw%3D%3D&md5=777736cb78d4c4ba50d9d60f1dfc769bCAS | 21988399PubMed |

[36]  Avbersek, J. et al. (2009) Diversity of Clostridium difficile in pigs and other animals in Slovenia. Anaerobe 15, 252–255.
Diversity of Clostridium difficile in pigs and other animals in Slovenia.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhsFShtL%2FO&md5=f9e79fc22d6d5530be28a0d2324dc370CAS | 19632350PubMed |

[37]  Hoffer, E. et al. (2010) Low occurrence of Clostridium difficile in fecal samples of healthy calves and pigs at slaughter and in minced meat in Switzerland. J. Food Prot. 73, 973–975.
| 1:STN:280:DC%2BC3czmtlGgtA%3D%3D&md5=62f3e267ea16153892aa1ed97a13bb34CAS | 20501051PubMed |

[38]  Costa, M.C. et al. (2011) Epidemiology of Clostridium difficile on a veal farm: prevalence, molecular characterization and tetracycline resistance. Vet. Microbiol. 152, 379–384.
Epidemiology of Clostridium difficile on a veal farm: prevalence, molecular characterization and tetracycline resistance.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhtVKltbrN&md5=015fe03bfe4889239f6db83bd798d7dfCAS | 21641131PubMed |

[39]  Stabler, R.A. et al. (2012) Macro and micro diversity of Clostridium difficile isolates from diverse sources and geographical locations. PLoS One 7, e31559.
Macro and micro diversity of Clostridium difficile isolates from diverse sources and geographical locations.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XjvFaisLc%3D&md5=c3fa314943a44229d298198679668885CAS | 22396735PubMed |

[40]  Androga, G.O. et al. (2015) Evaluation of the Cepheid Xpert C. difficile/Epi and meridian bioscience illumigene C. difficile assays for detecting Clostridium difficile ribotype 033 strains. J. Clin. Microbiol. 53, 973–975.
Evaluation of the Cepheid Xpert C. difficile/Epi and meridian bioscience illumigene C. difficile assays for detecting Clostridium difficile ribotype 033 strains.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXjsVyiu7s%3D&md5=aa1bdf1610584df5b84311ab46443e21CAS | 25520452PubMed |

[41]  Knight, D.R. et al. (2013) Prevalence of gastrointestinal Clostridium difficile carriage in Australian sheep and lambs. Appl. Environ. Microbiol. 79, 5689–5692.
Prevalence of gastrointestinal Clostridium difficile carriage in Australian sheep and lambs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhsVSgtrzP&md5=328c9382e171409f7874c56321e75c64CAS | 23851101PubMed |

[42]  Knight, D.R. et al. (2014) Laboratory detection of Clostridium difficile in piglets in Australia. J. Clin. Microbiol. 52, 3856–3862.
Laboratory detection of Clostridium difficile in piglets in Australia.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXitVyitb7J&md5=5ad4f85dce9f2944903368acdf27d9f7CAS | 25122859PubMed |