Testing mitochondrial marker efficacy for DNA barcoding in spiders: a test case using the dwarf spider genus Oedothorax (Araneae : Linyphiidae : Erigoninae)
Lara Lopardo A B and Gabriele Uhl A
+ Author Affiliations
- Author Affiliations
A Allgemeine und Systematische Zoologie, Zoologisches Institut und Museum, Ernst-Moritz-Arndt-Universität, Anklamer Strasse 20, D-17489 Greifswald, Germany.
B Corresponding author. Email: laralopardo@gmail.com
Invertebrate Systematics 28(5) 501-521 https://doi.org/10.1071/IS14017
Submitted: 3 April 2014 Accepted: 12 July 2014 Published: 13 November 2014
Abstract
The present study focusses on comparatively assessing the efficacy for DNA barcoding of the two most commonly used mitochondrial markers (cox1 and 16S) in a genus of erigonine spiders. In total, 53 specimens representing five species, including four multi-sampled species, were sampled from several European localities. Initial evaluation of species monophyly was performed through parsimony and Bayesian phylogenetic analyses. Efficacy of mitochondrial markers was tested using operational (including distance-, tree-based measures and Barcode Gap) and evolutionary criteria (using the General Mixed Yule-coalescent Model) for species delimitation. We propose that the cox1 marker can potentially overestimate analyses of biodiversity and thus might not be the preferred marker for DNA species identification and delimitation methods in Oedothorax. Instead, our results suggest that the 16S marker appears to be a promising candidate for such endeavour. Evaluating the contribution and suitability of markers to the re-identification of species, measured by their recovery of well established morphological species, is critical for future studies and for reliable results in species identification in spiders.
Additional keywords: biodiversity, species boundaries, mitochondrial DNA, molecular taxonomy.
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