Wild populations of Sydney rock oysters differ in their proteomic responses to elevated carbon dioxide
E. L. Thompson A B F , L. Parker C , V. Amaral A D , M. J. Bishop A B , W. A. O’Connor E and D. A. Raftos A
+ Author Affiliations
- Author Affiliations
A Department of Biological Sciences, Macquarie University, North Ryde, NSW 2109, Australia.
B Sydney Institute of Marine Science, Building 19, Chowder Bay Road, Mosman, NSW 2088, Australia.
C School of Natural Sciences, College of Health and Science, University of Western Sydney, Penrith South DC, Sydney, NSW 1797, Australia.
D MARE – Marine and Environmental Sciences Centre, Faculdade de Ciências da Universidade de Lisboa, Campo Grande, PT-1749-016 Lisboa, Portugal.
E NSW Department of Primary Industries, Port Stephens Fisheries Research Institute, Taylors Beach, NSW 2316, Australia.
F Corresponding author. Email: emma.thompson@mq.edu.au
Marine and Freshwater Research 67(12) 1964-1972 https://doi.org/10.1071/MF15320
Submitted: 10 December 2014 Accepted: 5 November 2015 Published: 8 January 2016
Abstract
This study tested the proteomic responses of three spatially distinct Sydney rock oyster populations to elevated pCO2. Oysters were collected from environmentally different sites, two chronically affected by acid sulfate soil. Oysters from each of the three populations were exposed to ambient (380 µatm) or elevated (856 and 1500 µatm) pCO2 for 4 weeks. Subsequent proteomic analysis from haemolymph revealed that (1) there were differences between the proteomes of the three populations after exposure to ambient pCO2, and (2) the different oyster populations mounted significantly different responses to elevated pCO2. Proteins that differed significantly in concentration between pCO2 treatments fell into five broad functional categories: energy metabolism, cellular stress responses, the cytoskeleton, protein synthesis and the extracellular matrix. This is consistent with the hypothesis that environmental stress in oysters leads to a generic response involving increased mitochondrial energy production to maintain cellular homeostasis. Proteins involved in the cytoskeleton and energy metabolism were the most differentially expressed and were seen in all three oyster populations. Differences between populations in their proteomic responses suggested that the local environments from which oysters originate may affect their capacity to respond to ocean acidification.
Additional keywords: ocean acidification, proteomics, Sydney rock oysters.
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