Short- and long-term effects of surface fires on heat stress protein content in Scots pine needles
N. E. Korotaeva A D , I. G. Gette B , N. V. Pacharkova B , I. V. Kosov C and G. B. Borovskii A
+ Author Affiliations
- Author Affiliations
A Siberian Institute of Plant Physiology and Biochemistry, Irkutsk Science Center, Siberian Branch, Russian Academy of Sciences, RU-664033 Irkutsk, Russian Federation.
B Siberian Federal University, RU-660041 Krasnoyarsk, Russian Federation.
C Sukachev Institute of Forest, Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences, RU-660036 Krasnoyarsk, Russian Federation.
D Corresponding author. Email: knev73@yandex.ru
International Journal of Wildland Fire 30(12) 978-989 https://doi.org/10.1071/WF20084
Submitted: 5 June 2020 Accepted: 8 September 2021 Published: 22 October 2021
Abstract
Plants can minimise the damaging effects of high temperatures through numerous protective mechanisms; however, it is largely unknown how these mechanisms respond to extreme temperatures associated with wildfire. We investigated the effect of experimental burning (EB) on the accumulation of stress heat shock proteins (Hsps), which are one of the factors of thermotolerance in plants, in the needles of Scots pine (Pinus sylvestris L.). Previous fire exposure led not only to short- and long-term changes in the content of stress proteins in needles but also to changes in the accumulation of these proteins in response to reheating. The content of Hsp 101, Hsp 70 and Hsp 17.6 in the needles increased on the second day after EB (short-term effect of fire). Three years after EB, the content of Hsps in the fire-exposed needles was lower compared with the control needles. When these needles were subjected to the heat stress test at 45°C, the content of Hsps increased, whereas the content of Hsps in control needles decreased. Our results suggest that Scots pine needles retain a fairly long-term ‘stress memory’, expressed through proteomic defence mechanisms, to wildfire heat-induced damage.
Keywords: Scots pine, needles, experimental burning, wildfire, post-fire impacts, heat shock proteins, Hsp, fire severity.
References
Alekseev VA (1989) Diagnostics of the vital state of trees and stands. Russian Journal of Forest Science 4, 51–57. [In Russian]Allakhverdiev SI, Kreslavski VD, Klimov VV, Los DA, Carpentier R, Mohanty P (2008) Heat stress: an overview of molecular responses in photosynthesis. Photosynthesis Research 98, 541–550.
| Heat stress: an overview of molecular responses in photosynthesis.Crossref | GoogleScholarGoogle Scholar | 18649006PubMed |
Biswal B, Joshi PN, Raval MK, Biswal UC (2011) Photosynthesis, a global sensor of environmental stress in green plants: stress signalling and adaptation. Current Science 101, 47–56.
Bita CE, Gerats T (2013) Plant tolerance to high temperature in a changing environment: scientific fundamentals and production of heat stress-tolerant crops. Frontiers in Plant Science 4, 273
| Plant tolerance to high temperature in a changing environment: scientific fundamentals and production of heat stress-tolerant crops.Crossref | GoogleScholarGoogle Scholar | 23914193PubMed |
Boston RS, Viitanen PV, Vierling E (1996) Molecular chaperones and protein folding in plants. Plant Molecular Biology 32, 191–222.
| Molecular chaperones and protein folding in plants.Crossref | GoogleScholarGoogle Scholar | 8980480PubMed |
Bugaeva KS (2009) Typological structure of tree stands in the Pogorelsky pine forest, Krasnoyarsk forest-steppe. Russian Journal of Forest Science 6, 46–53. [In Russian]
Bulgakov VP, Wu H-C, Jinn T-L (2019) Coordination of ABA and chaperone signaling in plant stress responses. Trends in Plant Science 24, 636–651.
| Coordination of ABA and chaperone signaling in plant stress responses.Crossref | GoogleScholarGoogle Scholar | 31085125PubMed |
Colombo SJ, Timmer VR (1992) Limits of tolerance to high temperatures causing direct and indirect damage to black spruce. Tree Physiology 11, 95–104.
| Limits of tolerance to high temperatures causing direct and indirect damage to black spruce.Crossref | GoogleScholarGoogle Scholar | 14969970PubMed |
Ensminger I, Sveshnikov D, Campbell DA, Funk C, Jansson S, Lloyd J, Shibistova O, Öquist G (2004) Intermittent low temperatures constrain spring recovery of photosynthesis in boreal Scots pine forests. Global Change Biology 10, 995–1008.
| Intermittent low temperatures constrain spring recovery of photosynthesis in boreal Scots pine forests.Crossref | GoogleScholarGoogle Scholar |
Federal Forestry Agency (2015) Guide to planning, organization and maintaining of inspections of forest pathology. Order of Federal Forestry Agency of 15 May 2015, no. 159, p. 20. Available at http://www.forestforum.ru/info/laws/lp_159.pdf [In Russian]
Furniss TJ, Larson AJ, Kane VR, Lutz JA (2019) Multi-scale assessment of post-fire tree mortality models. International Journal of Wildland Fire 28, 46–61.
| Multi-scale assessment of post-fire tree mortality models.Crossref | GoogleScholarGoogle Scholar |
Gavrilenko VF, Zhigalova TV (Eds) (2003) ‘Large workshop in photosynthesis.’ (Academy: Moscow, Russian Federation) [In Russian]
Gette IG, Pakharkova NV, Kosov IV, Bezkorovaynaya IN (2017) Fluorescence methods for estimation of post-fire response of pine needles. Folia Forestalia Polonica – A, Forestry 59, 249–257.
| Fluorescence methods for estimation of post-fire response of pine needles.Crossref | GoogleScholarGoogle Scholar |
Girs GI (Ed.) (1982) ‘Physiology of weakened trees.’ (Nauka: Novosibirsk, Russian Federation) [In Russian]
Guo M, Liu J-H, Ma X, Luo D-X, Gong Z-H, Lu M-H (2016) The plant heat stress transcription factors (HSFs): structure, regulation, and function in response to abiotic stresses. Frontiers in Plant Science 7, 114
| The plant heat stress transcription factors (HSFs): structure, regulation, and function in response to abiotic stresses.Crossref | GoogleScholarGoogle Scholar | 26904076PubMed |
Hartl FU (1996) Molecular chaperones in cellular protein folding. Nature 381, 571–579.
| Molecular chaperones in cellular protein folding.Crossref | GoogleScholarGoogle Scholar | 8637592PubMed |
Heckathorn SA, Downs CA, Sharkey TD, Coleman JS (1998) The small, methionine-rich chloroplast heat-shock protein protects photosystem II electron transport during heat stress. Plant Physiology 116, 439–444.
| The small, methionine-rich chloroplast heat-shock protein protects photosystem II electron transport during heat stress.Crossref | GoogleScholarGoogle Scholar | 9449851PubMed |
Huang B, Xu C (2008) Identification and characterization of proteins associated with plant tolerance to heat stress. Journal of Integrative Plant Biology 50, 1230–1237.
| Identification and characterization of proteins associated with plant tolerance to heat stress.Crossref | GoogleScholarGoogle Scholar | 19017110PubMed |
Keeling PL, Banisadr R, Barone L, Wasserman BP, Singletary GW (1994) Effect of temperature on enzymes in the pathway of starch biosynthesis in developing wheat and maize grain. Australian Journal of Plant Physiology 21, 807–827.
| Effect of temperature on enzymes in the pathway of starch biosynthesis in developing wheat and maize grain.Crossref | GoogleScholarGoogle Scholar |
Jacob P, Hirt H, Bendahmane A (2017) The heat-shock protein/chaperone network and multiple stress resistance. Plant Biotechnology Journal 15, 405–414.
| The heat-shock protein/chaperone network and multiple stress resistance.Crossref | GoogleScholarGoogle Scholar | 27860233PubMed |
Khan MS, Yu X, Kikuchi A, Asahina M, Watanabe KN (2009) Genetic engineering of glycine betaine biosynthesis to enhance abiotic stress tolerance in plants. Plant Biotechnology 26, 125–134.
| Genetic engineering of glycine betaine biosynthesis to enhance abiotic stress tolerance in plants.Crossref | GoogleScholarGoogle Scholar |
Kolb PF, Robberecht R (1996) High temperature and drought stress effects on survival of Pinus ponderosa seedlings. Tree Physiology 16, 665–672.
| High temperature and drought stress effects on survival of Pinus ponderosa seedlings.Crossref | GoogleScholarGoogle Scholar | 14871688PubMed |
Korotaeva NE, Oskorbina MV, Kopytova LD, Suvorova GG, Borovskii GB, Voinikov VK (2012) Variations in the content of stress proteins in the needles of Scots pine (Pinus sylvestris L) within an annual cycle. Journal of Forest Research 17, 89–97.
| Variations in the content of stress proteins in the needles of Scots pine (Pinus sylvestris L) within an annual cycle.Crossref | GoogleScholarGoogle Scholar |
Korotaeva NE, Gette IG, Kosov IV, Pakharkova NV, Borovsky GB (2017) Heat shock proteins and photosynthetic activity of needles of ordinary pine in post-fire period. The Bulletin of KrasGAU 10, 79–87. [In Russian]
Korotaeva NE, Ivanova MV, Suvorova GG, Borovskii GB (2018) The impact of the environmental factors on the photosynthetic activity of Scots pine (Pinus sylvestris) in spring and in autumn in the region of eastern Siberia. Journal of Forestry Research 29, 1465–1473.
| The impact of the environmental factors on the photosynthetic activity of Scots pine (Pinus sylvestris) in spring and in autumn in the region of eastern Siberia.Crossref | GoogleScholarGoogle Scholar |
Lämke J, Baurle I (2017) Epigenetic and chromatin-based mechanisms in environmental stress adaptation and stress memory in plants. Genome Biology 18, 124
| Epigenetic and chromatin-based mechanisms in environmental stress adaptation and stress memory in plants.Crossref | GoogleScholarGoogle Scholar | 28655328PubMed |
Larkindale J, Hall JD, Knight MR, Vierling E (2005) Heat stress phenotypes of Arabidopsis mutants implicate multiple signaling pathways in the acquisition of thermotolerance. Plant Physiology 138, 882–897.
| Heat stress phenotypes of Arabidopsis mutants implicate multiple signaling pathways in the acquisition of thermotolerance.Crossref | GoogleScholarGoogle Scholar | 15923322PubMed |
Maxwell K, Johnson GN (2000) Chlorophyll fluorescence – a practical guide. Journal of Experimental Botany 51, 659–668.
| Chlorophyll fluorescence – a practical guide.Crossref | GoogleScholarGoogle Scholar | 10938857PubMed |
Michaletz ST (2018) Xylem dysfunction in fires: towards a hydraulic theory of plant responses to multiple disturbance stressors. New Phytologist 217, 1391–1393.
| Xylem dysfunction in fires: towards a hydraulic theory of plant responses to multiple disturbance stressors.Crossref | GoogleScholarGoogle Scholar |
Michaletz ST, Johnson EA (2006) A heat transfer model of crown scorch in forest fires. Canadian Journal of Forest Research 36, 2839–2851.
| A heat transfer model of crown scorch in forest fires.Crossref | GoogleScholarGoogle Scholar |
Perevoznikova VD, Ivanova GA, Ivanov VA, Kovaleva NM, Konard SG (2005) Species composition and structure of the living surface cover in pine stands after controlled burning. Contemporary Problems of Ecology 1, 135–141.
Ponomarev EI, Kharuk VI (2016) Wildfire occurrence in forests of the Altai–Sayan region under current climate changes. Contemporary Problems of Ecology 9, 29–36.
| Wildfire occurrence in forests of the Altai–Sayan region under current climate changes.Crossref | GoogleScholarGoogle Scholar |
Ruelland E, Zachowski A (2010) How plants sense temperature. Environmental and Experimental Botany 69, 225–232.
| How plants sense temperature.Crossref | GoogleScholarGoogle Scholar |
Schonfeld MA, Johnson RC, Carver BF, Mornhinweg DW (1988) Water relations in winter wheat as drought resistance indicator. Crop Science 28, 526–531.
| Water relations in winter wheat as drought resistance indicator.Crossref | GoogleScholarGoogle Scholar |
Shvidenko AZ, Schepaschenko DG (2013) Climate change and wildfires in Russia. Contemporary Problems of Ecology 6, 683–692.
| Climate change and wildfires in Russia.Crossref | GoogleScholarGoogle Scholar |
Smith AMS, Sparks AM, Kolden CA, Abatzoglou JT, Talhelm AF, Johnson DM, Boschetti L, Lutz JA, Apostol KG, Yedinak KM, Tinkham WT, Kremens RJ (2016) Towards a new paradigm in fire severity research using dose–response experiments. International Journal of Wildland Fire 25, 158–166.
| Towards a new paradigm in fire severity research using dose–response experiments.Crossref | GoogleScholarGoogle Scholar |
Smith AMS, Talhelm AF, Johnson DM, Sparks AM, Kolden CA, Yedinak KM, Apostol KG, Tinkham WT, Abatzoglou JT, Lutz JA, Davis AS, Pregitzer KS, Adams HD, Kremens RL (2017) Effects of fire radiative energy density dose on Pinus contorta and Larix occidentalis seedling physiology and mortality. International Journal of Wildland Fire 26, 82–94.
| Effects of fire radiative energy density dose on Pinus contorta and Larix occidentalis seedling physiology and mortality.Crossref | GoogleScholarGoogle Scholar |
Sparks AM, Smith AMS, Talhelm AF, Kolden CA, Yedinak KM, Johnson DM (2017) Impacts of fire radiative flux on mature Pinus ponderosa growth and vulnerability to secondary mortality agents. International Journal of Wildland Fire 26, 95–106.
| Impacts of fire radiative flux on mature Pinus ponderosa growth and vulnerability to secondary mortality agents.Crossref | GoogleScholarGoogle Scholar |
Sparks AM, Kolden CA, Smith AMS, Boschetti L, Johnson DM, Cochrane MA (2018) Fire intensity impacts on post-fire temperate coniferous forest net primary productivity. Biogeosciences 15, 1173–1183.
| Fire intensity impacts on post-fire temperate coniferous forest net primary productivity.Crossref | GoogleScholarGoogle Scholar |
Steady WD, Feltrin RP, Johnson DM, Sparks AM, Kolden CA, Talhelm AF, Lutz JA, Boschetti L, Hudak AT, Nelson AS, Smith AMS (2019) The survival of Pinus ponderosa saplings subjected to increasing levels of fire behavior and impacts on post-fire growth. Fire 2, 23
| The survival of Pinus ponderosa saplings subjected to increasing levels of fire behavior and impacts on post-fire growth.Crossref | GoogleScholarGoogle Scholar |
Sudachkova NE, Romanova LI, Astrakhantseva NV, Novoselova MV (2017) Thermostability of antioxidant enzymes in tissues of Scots Pine in heat shock conditions. Siberian Journal of Forest Science 1, 4–14. [In Russian]
Sung DY, Vierling E, Guy CL (2001) Comprehensive expression profile analysis of the Arabidopsis Hsp 70 gene family. Plant Physiology 126, 789–800.
| Comprehensive expression profile analysis of the Arabidopsis Hsp 70 gene family.Crossref | GoogleScholarGoogle Scholar | 11402207PubMed |
Teskey R, Wertin T, Bauweraerts I, Ameye M, McGuire MA, Steppe K (2015) Responses of tree species to heat waves and extreme heat events. Plant, Cell & Environment 38, 1699–1712.
| Responses of tree species to heat waves and extreme heat events.Crossref | GoogleScholarGoogle Scholar |
Tsvetkov PA (2006) The height of the tree trunk charring as a diagnostic sign. Conifers of the Boreal Area 23, 132–137. [In Russian]
Valendik EN, Brissette JC, Kisilyakhov YeK, Lasko RJ, Verkhovets SV, Eubanks ST, Kosov IV, Lantukh AY (2006) An experimental burn to restore a moth-killed boreal conifer forest, Krasnoyarsk region, Russia. Mitigation and Adaptation Strategies for Global Change 11, 883–896.
| An experimental burn to restore a moth-killed boreal conifer forest, Krasnoyarsk region, Russia.Crossref | GoogleScholarGoogle Scholar |
Wang W, Vinocur B, Shoseyov O, Altman A (2004) Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response. Trends in Plant Science 9, 244–252.
| Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response.Crossref | GoogleScholarGoogle Scholar | 15130550PubMed |
Welling A, Palva ET (2006) Molecular control of cold acclimation in trees. Physiologia Plantarum 127, 167–181.
| Molecular control of cold acclimation in trees.Crossref | GoogleScholarGoogle Scholar |
Wirth C, Czimczik CI, Schulze E-D (2002) Beyond annual budgets: carbon flux at different temporal scales in fire-prone Siberian Scots pine forests. Tellus 54B, 611–630.
| Beyond annual budgets: carbon flux at different temporal scales in fire-prone Siberian Scots pine forests.Crossref | GoogleScholarGoogle Scholar |
Woolley T, Shaw DC, Ganio LM, Fitzgerald S (2012) A review of logistic regression models used to predict post-fire tree mortality of western North American conifers. International Journal of Wildland Fire 21, 1–35.
| A review of logistic regression models used to predict post-fire tree mortality of western North American conifers.Crossref | GoogleScholarGoogle Scholar |
Ziȩtkiewicz S, Krzewska J, Liberek K (2004) Successive and synergistic action of the Hsp 70 and Hsp 100 chaperones in protein disaggregation. Journal of Biological Chemistry 279, 44376–44383.
| Successive and synergistic action of the Hsp 70 and Hsp 100 chaperones in protein disaggregation.Crossref | GoogleScholarGoogle Scholar |
Żwirowski S, Kłosowska A, Obuchowski I, Nillegoda NB, Piróg A, Zieztkiewicz S, Bukau B, Mogk A, Liberek K (2017) Hsp 70 displaces small heat shock proteins from aggregates to initiate protein refolding. The EMBO Journal 36, 783–796.
| Hsp 70 displaces small heat shock proteins from aggregates to initiate protein refolding.Crossref | GoogleScholarGoogle Scholar | 28219929PubMed |
