Introduction of routine polymerase chain reaction testing for Neisseria gonorrhoeae in a community laboratory
Arlo Upton A B , Janet Wilson A and Liselle Bissessor AA Labtests, 27-41 Carbine Road, Mt Wellington 1060, New Zealand.
B Corresponding author. Email: arlo.upton@labtests.co.nz
Sexual Health 10(4) 387-388 https://doi.org/10.1071/SH12213
Submitted: 9 November 2012 Accepted: 5 February 2013 Published: 17 May 2013
Abstract
We introduced polymerase chain reaction (PCR) testing for Neisseria gonorrhoeae (NG) on the Cobas 4800 CT/NG assay for all samples received with a Chlamdyia trachomatis request in March 2012. From 1 March 2012 to 30 June 2012, all PCR-positive/culture-negative specimens had additional testing at another assay. A total of 40053 tests were performed. The estimated specificity and positive predictive value were 99.9% and 97.1%, respectively; thus routine additional testing is not required.
Additional keywords: diagnosis, gonorrhoea, nucleic acid amplification testing.
References
[1] Workowski KA, Berman SM. Centers for Disease Control and Prevention sexually transmitted disease treatment guidelines. Clin Infect Dis 2011; 53 S59–63.| Centers for Disease Control and Prevention sexually transmitted disease treatment guidelines.Crossref | GoogleScholarGoogle Scholar | 22080270PubMed |
[2] Tabrizi SN, Unemo M, Limnios AE, Hogan TR, Hjelmevoll S-O, Garland SM, et al Evaluation of six commercial nucleic acid amplification tests for detection of Neisseria gonorrhoeae and other Neisseria species. J Clin Microbiol 2011; 49 3610–5.
| Evaluation of six commercial nucleic acid amplification tests for detection of Neisseria gonorrhoeae and other Neisseria species.Crossref | GoogleScholarGoogle Scholar | 21813721PubMed |
[3] Van Der Pol B, Liesenfeld O, Williams JA, Taylor SN, Lillis RA, Body BA, et al Performance of the Cobas CT/NG test compared to the Aptima AC2 and Viper CTQ/GCQ assays for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J Clin Microbiol 2012; 50 2244–9.
| Performance of the Cobas CT/NG test compared to the Aptima AC2 and Viper CTQ/GCQ assays for detection of Chlamydia trachomatis and Neisseria gonorrhoeae.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhtFCmsL7E&md5=f97a5ba2261b558da52d5a1fe66298e6CAS | 22518864PubMed |
[4] Rockett R, Goire N, Limnios A, Turra M, Higgens G, Lambert SB, et al Evaluation of the Cobas 4800 CT/NG test for detecting Chlamydia trachomatis and Neisseria gonorrhoeae. Sex Transm Infect 2010; 86 470–3.
| Evaluation of the Cobas 4800 CT/NG test for detecting Chlamydia trachomatis and Neisseria gonorrhoeae.Crossref | GoogleScholarGoogle Scholar | 20719957PubMed |
[5] Whiley DM, Garland SM, Harnett G, Lum G, Smith DW, Tabrizi SN, et al Exploring “best practice” for nucleic acid detection of Neisseria gonorrhoeae. Sex Health 2008; 5 17–23.
| Exploring “best practice” for nucleic acid detection of Neisseria gonorrhoeae.Crossref | GoogleScholarGoogle Scholar | 18361850PubMed |
