Register      Login
Sexual Health Sexual Health Society
Publishing on sexual health from the widest perspective
RESEARCH ARTICLE

The prevalence of Trichomonas vaginalis detected by wet mount and polymerase chain reaction in Sydney women

Ivy Kwon A , Anna McNulty B C and Phillip Read B D E
+ Author Affiliations
- Author Affiliations

A University of New South Wales, Sydney, NSW 2052, Australia.

B Sydney Sexual Health Centre, GPO Box 1614, Sydney, NSW 2001, Australia.

C School of Public Health and Community Medicine, University of New South Wales, Sydney, NSW, Australia.

D The Kirby Institute, University of New South Wales, Sydney, NSW, Australia.

E Corresponding author. Email: phillip.read@sesiahs.health.nsw.gov.au

Sexual Health 10(4) 385-386 https://doi.org/10.1071/SH12201
Submitted: 29 November 2012  Accepted: 13 January 2013   Published: 24 April 2013

Abstract

Objectives: Although Trichomonas vaginalis (TV) has a low profile in urban Australia, local data has estimated the prevalence in women to be 10 times higher when using polymerase chain reaction (PCR) versus wet mount microscopy (4.8% v. 0.4%). Our aim was to determine the prevalence of TV in Sydney women using both wet mount and PCR. Methods: A cross-sectional study was conducted of women requiring sexually transmissible infection screening at the Sydney Sexual Health Centre. Vaginal swabs were examined for TV using PCR and wet mount microscopy. Results: In total, 781 of 1263 eligible women were tested; 3 out of 781 tested positive by PCR and 1 out of 781 by wet mount, giving a prevalence of 0.38% (95% confidence interval (CI): 0.14–1.12%) and 0.13% (95% CI: 0.03–0.71%) respectively. There was not enough power to compare PCR and wet mount. Conclusions: The results of this analysis indicate that in our female urban population, TV is a very rare sexually transmissible infection,with 0.38% prevalence, and routine screening by PCR is not indicated.

Additional keywords: Australia, epidemiology, microscopy.


References

[1]  World Health Organization (WHO). Global prevalence and incidence of selected curable sexually transmitted diseases: overview and estimates. Geneva: WHO; 2001.

[2]  Bradshaw C. Vaginal symptoms. In Russell D, Bradford D, Fairley C, editors. Sexual health medicine, 2nd ed. Melbourne: IP Communications; 2011. pp.88–106.

[3]  Wiese W, Patel SR, Patel SC, Ohl CA, Estrada CA. A meta-analysis of the Papanicolaou smear and wet mount for the diagnosis of vaginal trichomoniasis. Am J Med 2000; 108 301–8.
A meta-analysis of the Papanicolaou smear and wet mount for the diagnosis of vaginal trichomoniasis.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3cvmt1KksA%3D%3D&md5=1423a8debb372d135f5afa35ae3c8d50CAS | 11014723PubMed |

[4]  Caliendo AM, Jordan JA, Green AM, Ingersoll J, Diclemente RJ, Wingood GM. Real-time PCR improves detection of Trichomonas vaginalis infection compared with culture using self-collected vaginal swabs. Infect Dis Obstet Gynecol 2005; 13 145–50.
Real-time PCR improves detection of Trichomonas vaginalis infection compared with culture using self-collected vaginal swabs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtVWitL3F&md5=259363ce72aef83ded3f6ecaa0969446CAS | 16126499PubMed |

[5]  Uddin RN, Ryder N, McNulty AM, Wray L, Donovan B. Trichomonas vaginalis infection among women in a low prevalence setting. Sex Health 2011; 8 65–8.
Trichomonas vaginalis infection among women in a low prevalence setting.Crossref | GoogleScholarGoogle Scholar | 21371384PubMed |

[6]  Lusk MJ, Naing Z, Rayner B, Rismanto N, McIver CJ, Cumming RG, et al Trichomonas vaginalis: underdiagnosis in urban Australia could facilitate re-emergence. Sex Transm Infect 2010; 86 227–30.
Trichomonas vaginalis: underdiagnosis in urban Australia could facilitate re-emergence.Crossref | GoogleScholarGoogle Scholar | 19880969PubMed |

[7]  Marrone J, Fairley CK, Saville M, Bradshaw C, Bowden FJ, Horvath LB, et al Temporal associations with declining Trichomonas vaginalis diagnosis rates among women in the state of Victoria, Australia, 1947 to 2005. Sex Transm Dis 2008; 35 572–6.
Temporal associations with declining Trichomonas vaginalis diagnosis rates among women in the state of Victoria, Australia, 1947 to 2005.Crossref | GoogleScholarGoogle Scholar | 18354342PubMed |

[8]  Ryder N, Woods H, McKay K, Giddings N, Lenton J, Little C, et al Trichomonas vaginalis prevalence increases with remoteness in rural and remote New South Wales, Australia. Sex Transm Dis 2012; 9
Trichomonas vaginalis prevalence increases with remoteness in rural and remote New South Wales, Australia.Crossref | GoogleScholarGoogle Scholar |

[9]  Bowden FJ, Paterson BA, Mein J, Savage J, Fairley CK, Garland SM, et al Estimating the prevalence of Trichomonas vaginalis, Chlamydia trachomatis, Neisseria gonorrhoeae, and human papillomavirus infection in indigenous women in northern Australia. Sex Transm Infect 1999; 75 431–4.
Estimating the prevalence of Trichomonas vaginalis, Chlamydia trachomatis, Neisseria gonorrhoeae, and human papillomavirus infection in indigenous women in northern Australia.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3c3hslOmtg%3D%3D&md5=7049c540c344132b4ba31cff78e862a6CAS | 10754952PubMed |