Molecular and morphological analyses support recognition of Prostanthera volucris (Lamiaceae), a new species from the Central Tablelands of New South Wales
Ryan P. O’Donnell
A B E * , Jeremy J. Bruhl A , Ian R. H. Telford A , Trevor C. Wilson B , Heidi C. Zimmer C , Guy M. Taseski D and Rose L. Andrew A *
+ Author Affiliations
- Author Affiliations
A Botany and N.C.W. Beadle Herbarium, School of Environmental and Rural Science, University of New England, Armidale, NSW 2351, Australia.
B National Herbarium of New South Wales, Australian Institute of Botanical Science, Royal Botanic Gardens and Domain Trust, Locked Bag 6002, Mount Annan, NSW 2567, Australia.
C Australian National Herbarium, Centre for Australian National Biodiversity Research, GPO Box 1700, Canberra, ACT 2601, Australia.
D John T. Waterhouse Herbarium, School of Biological, Earth and Environmental Sciences, University of New South Wales, Kensington, NSW 2052, Australia.
E Present address: Division of Ecology and Evolution, Research School of Biology, The Australian National University, Canberra, ACT 2600, Australia.
Australian Systematic Botany 36(1) 1-20 https://doi.org/10.1071/SB22017
Submitted: 20 June 2022 Accepted: 8 January 2023 Published: 8 February 2023
© 2023 The Author(s) (or their employer(s)). Published by CSIRO Publishing. This is an open access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND)
Abstract
Research into the systematics of Prostanthera recently revealed close evolutionary relationship among P. phylicifolia sens. str., the critically endangered P. gilesii, and a population of uncertain identity from the Central Tablelands of New South Wales (NSW), Australia. Previous analyses were unable to establish whether genetic boundaries separated these taxa. This study assessed species boundaries among these three taxa by using a combination of single-nucleotide polymorphisms (SNPs) sampled at the population-scale and multivariate analysis of morphological characters. Ordination, model-based clustering, F-statistics, neighbour-network analysis, phylogenetic analysis, and ancestry coefficient estimates all provided support for discrete genetic differences among the three taxa. Morphological phenetic analysis recovered congruent morphological clusters and identified a suite of corresponding diagnostic characters. This congruence of molecular and morphological evidence supports the presence of three independently evolving lineages, two of which correspond with the previously described P. gilesii and P. phylicifolia sens. str. The third taxon, represented by a single population from the Central Tablelands of NSW, is here described as P. volucris R.P.O’Donnell. A detailed description, diagnostic line drawings and photographs are provided. We evaluate P. volucris as satisfying criteria to be considered Critically Endangered.
Keywords: critically endangered, DArTseq, genotyping-by-sequencing, Lamiaceae, population genomics, species delimitation, systematics, taxonomy.
References
Atkins ZS, Amor MD, Clemann N, Chapple DG, While GM, Gardner MG, Haines ML, Harrisson KA, Schroder M, Robert KA (2020) Allopatric divergence drives the genetic structuring of an endangered alpine endemic lizard with a sky-island distribution. Animal Conservation 23, 104–118.| Allopatric divergence drives the genetic structuring of an endangered alpine endemic lizard with a sky-island distribution.Crossref | GoogleScholarGoogle Scholar |
Australian Plant Census (2021) APC: Prostanthera. In ‘The Australian National Species List: Vascular Plants’. APC (version 51411005). (Council of Heads of Australasian Herbaria) Available at https://biodiversity.org.au/nsl/services/rest/treeElement/51411005/51380123 [Verified 9 January 2023]
Australia’s Virtual Herbarium (2021) Occurrence records: Prostanthera phylicifolia, P. gilesii and P. sp. Evans Crown. In ‘The Australasian Virtual Herbarium’. (Atlas of Living Australia) Available at https://avh.ala.org.au/occurrences/search?q=%28taxa%3A%22Prostanthera+phylicifolia%22+OR+taxa%3A%22Prostanthera+gilesii%22+OR+raw_scientificName%3A%22Prostanthera+sp.+Evans+Crown%22%29#tab_mapView [Verified 9 January 2023]
Bean AR (2014) Four new Queensland species of Solanum L. allied to S. ellipticum R.Br. (Solanaceae). Austrobaileya 9, 216–228.
Belbin L, Collins A (2013) ‘PATN - Finding Patterns in Data.’ (Blatant Fabrications)
Bell N, Griffin PC, Hoffmann AA, Miller AD (2018) Spatial patterns of genetic diversity among Australian alpine flora communities revealed by comparative phylogenomics. Journal of Biogeography 45, 177–189.
| Spatial patterns of genetic diversity among Australian alpine flora communities revealed by comparative phylogenomics.Crossref | GoogleScholarGoogle Scholar |
Bentham G (1870) Labiatae. In ‘Flora Australiensis. Vol. 5’. (Eds G Bentham, F Mueller) pp. 70–137. (Reeve: London, UK)
Bezemer N, Krauss SL, Roberts DG, Hopper SD (2019) Conservation of old individual trees and small populations is integral to maintain species’ genetic diversity of a historically fragmented woody perennial. Molecular Ecology 28, 3339–3357.
| Conservation of old individual trees and small populations is integral to maintain species’ genetic diversity of a historically fragmented woody perennial.Crossref | GoogleScholarGoogle Scholar |
Bryant D, Moulton V (2004) Neighbor-net: an agglomerative method for the construction of phylogenetic networks. Molecular Biology and Evolution 21, 255–265.
| Neighbor-net: an agglomerative method for the construction of phylogenetic networks.Crossref | GoogleScholarGoogle Scholar |
Bull JK, Sands CJ, Garrick RC, Gardner MG, Tait NN, Briscoe DA, Rowell DM, Sunnucks P (2013) Environmental complexity and biodiversity: the multi-layered evolutionary history of a log-dwelling velvet worm in montane temperate Australia. PLoS One 8, e84559
| Environmental complexity and biodiversity: the multi-layered evolutionary history of a log-dwelling velvet worm in montane temperate Australia.Crossref | GoogleScholarGoogle Scholar |
Byrne M, Steane DA, Joseph L, Yeates DK, Jordan GJ, Crayn D, Aplin K, Cantrill DJ, Cook LG, Crisp MD, Keogh JS, Melville J, Moritz C, Porch N, Sniderman JMK, Sunnucks P, Weston PH (2011) Decline of a biome: evolution, contraction, fragmentation, extinction and invasion of the Australian mesic zone biota. Journal of Biogeography 38, 1635–1656.
| Decline of a biome: evolution, contraction, fragmentation, extinction and invasion of the Australian mesic zone biota.Crossref | GoogleScholarGoogle Scholar |
Carlson E, MacDonald AJ, Adamack A, McGrath T, Doucette LI, Osborne WS, Gruber B, Sarre SD (2016) How many conservation units are there for the endangered grassland earless dragons? Conservation Genetics 17, 761–774.
| How many conservation units are there for the endangered grassland earless dragons?Crossref | GoogleScholarGoogle Scholar |
Chappill JA, Ladiges PY (1992) Geographical variation in morphology of Eucalyptus mannifera and allied taxa. Australian Systematic Botany 5, 359–372.
| Geographical variation in morphology of Eucalyptus mannifera and allied taxa.Crossref | GoogleScholarGoogle Scholar |
Chapple DG, Keogh JS, Hutchinson MN (2005) Substantial genetic substructuring in southeastern and alpine Australia revealed by molecular phylogeography of the Egernia whitii (Lacertilia: Scincidae) species group. Molecular Ecology 14, 1279–1292.
| Substantial genetic substructuring in southeastern and alpine Australia revealed by molecular phylogeography of the Egernia whitii (Lacertilia: Scincidae) species group.Crossref | GoogleScholarGoogle Scholar |
Chifman J, Kubatko L (2014) Quartet inference from SNP data under the coalescent model. Bioinformatics 30, 3317–3324.
| Quartet inference from SNP data under the coalescent model.Crossref | GoogleScholarGoogle Scholar |
Chifman J, Kubatko L (2015) Identifiability of the unrooted species tree topology under the coalescent model with time-reversible substitution processes, site-specific rate variation, and invariable sites. Journal of Theoretical Biology 374, 35–47.
| Identifiability of the unrooted species tree topology under the coalescent model with time-reversible substitution processes, site-specific rate variation, and invariable sites.Crossref | GoogleScholarGoogle Scholar |
Collins TL, Schmidt-Lebuhn AN, Andrew RL, Telford IRH, Bruhl JJ (2022) There’s gold in them thar hills! Morphology and molecules delimit species in Xerochrysum (Asteraceae; Gnaphalieae) and reveal many new taxa. Australian Systematic Botany 35, 120–185.
| There’s gold in them thar hills! Morphology and molecules delimit species in Xerochrysum (Asteraceae; Gnaphalieae) and reveal many new taxa.Crossref | GoogleScholarGoogle Scholar |
Conn BJ (1984) A taxonomic revision of Prostanthera Labill. section Klanderia (F.v. Muell.) Benth. (Labiatae). Journal of the Adelaide Botanic Gardens 6, 207–348.
Conn BJ (1988) A taxonomic revision of Prostanthera Labill. section Prostanthera (Labiatae), 1. The species of the Northern Territory, South Australia and Western Australia. Nuytsia 6, 351–411.
Conn BJ, Wilson TC (2015) Two new species of Prostanthera (Lamiaceae) in New South Wales. Telopea 18, 463–474.
| Two new species of Prostanthera (Lamiaceae) in New South Wales.Crossref | GoogleScholarGoogle Scholar |
Conn BJ, Wilson TC, Henwood MJ, Proft K (2013) Circumscription and phylogenetic relationships of Prostanthera densa and P. marifolia (Lamiaceae). Telopea 15, 149–164.
| Circumscription and phylogenetic relationships of Prostanthera densa and P. marifolia (Lamiaceae).Crossref | GoogleScholarGoogle Scholar |
Conn BJ, Henwood MJ, Proft K, Wilson TC (2016) Molecular phylogenetics reveals a new species of Prostanthera from tropical Queensland with links to more southerly taxa. Telopea 19, 13–22.
| Molecular phylogenetics reveals a new species of Prostanthera from tropical Queensland with links to more southerly taxa.Crossref | GoogleScholarGoogle Scholar |
Conn BJ, Henwood MJ, Proft KM, Scott JA, Wilson TC, Howes RS (2021) An integrative taxonomic approach resolves the Prostanthera lasianthos (Lamiaceae) species complex. Australian Systematic Botany 34, 438–476.
| An integrative taxonomic approach resolves the Prostanthera lasianthos (Lamiaceae) species complex.Crossref | GoogleScholarGoogle Scholar |
Copeland LM, Bruhl JJ, Craven LA, Brubaker CL (2007) Phenetic analyses of Homoranthus (Myrtaceae: Chamelaucieae) on the basis of morphology. Australian Systematic Botany 20, 417–427.
| Phenetic analyses of Homoranthus (Myrtaceae: Chamelaucieae) on the basis of morphology.Crossref | GoogleScholarGoogle Scholar |
Cruz VMV, Kilian A, Dierig DA (2013) Development of DArT marker platforms and genetic diversity assessment of the US collection of the new oilseed crop Lesquerella and related species. PLoS One 8, e64062
| Development of DArT marker platforms and genetic diversity assessment of the US collection of the new oilseed crop Lesquerella and related species.Crossref | GoogleScholarGoogle Scholar |
Dayrat B (2005) Towards integrative taxonomy. Biological Journal of the Linnean Society 85, 407–415.
| Towards integrative taxonomy.Crossref | GoogleScholarGoogle Scholar |
de Queiroz K (2007) Species concepts and species delimitation. Systematic Biology 56, 879–886.
| Species concepts and species delimitation.Crossref | GoogleScholarGoogle Scholar |
de Salas MF, Schmidt-Lebuhn AN (2018) Integrative approach resolves the taxonomy of the Ozothamnus ledifolius (Asteraceae: Gnaphaliae) species complex in Tasmania, Australia. Phytotaxa 358, 117–138.
| Integrative approach resolves the taxonomy of the Ozothamnus ledifolius (Asteraceae: Gnaphaliae) species complex in Tasmania, Australia.Crossref | GoogleScholarGoogle Scholar |
Endo Y, Nash M, Hoffmann AA, Slatyer R, Miller AD (2015) Comparative phylogeography of alpine invertebrates indicates deep lineage diversification and historical refugia in the Australian Alps. Journal of Biogeography 42, 89–102.
| Comparative phylogeography of alpine invertebrates indicates deep lineage diversification and historical refugia in the Australian Alps.Crossref | GoogleScholarGoogle Scholar |
Fernández-Mazuecos M, Mellers G, Vigalondo B, Sáez L, Vargas P, Glover BJ (2017) Resolving recent plant radiations: power and robustness of genotyping-by-sequencing. Systematic Biology 67, 250–268.
| Resolving recent plant radiations: power and robustness of genotyping-by-sequencing.Crossref | GoogleScholarGoogle Scholar |
Fitzsimons JA, Michael DR (2017) Rocky outcrops: a hard road in the conservation of critical habitats. Biological Conservation 211, 36–44.
| Rocky outcrops: a hard road in the conservation of critical habitats.Crossref | GoogleScholarGoogle Scholar |
Freeland JR, Petersen S, Kirk H (2011) ‘Molecular Ecology.’ (Wiley-Blackwell: Hoboken, NJ, USA)
Frichot E, François O (2015) LEA: an R package for landscape and ecological association studies. Methods in Ecology and Evolution 6, 925–929.
| LEA: an R package for landscape and ecological association studies.Crossref | GoogleScholarGoogle Scholar |
Garrick RC, Sands CJ, Rowell DM, Tait NN, Greenslade P, Sunnucks P (2004) Phylogeography recapitulates topography: very fine-scale local endemism of a saproxylic ‘giant’ springtail at Tallaganda in the Great Dividing Range of south-east Australia. Molecular Ecology 13, 3329–3344.
| Phylogeography recapitulates topography: very fine-scale local endemism of a saproxylic ‘giant’ springtail at Tallaganda in the Great Dividing Range of south-east Australia.Crossref | GoogleScholarGoogle Scholar |
Garrick RC, Sands CJ, Rowell DM, Hillis DM, Sunnucks P (2007) Catchments catch all: long-term population history of a giant springtail from the southeast Australian highlands – a multigene approach. Molecular Ecology 16, 1865–1882.
| Catchments catch all: long-term population history of a giant springtail from the southeast Australian highlands – a multigene approach.Crossref | GoogleScholarGoogle Scholar |
Garrick RC, Rowell DM, Sunnucks P (2012) Phylogeography of saproxylic and forest floor invertebrates from Tallaganda, south-eastern Australia. Insects 3, 270–294.
| Phylogeography of saproxylic and forest floor invertebrates from Tallaganda, south-eastern Australia.Crossref | GoogleScholarGoogle Scholar |
Georges A, Gruber B, Pauly GB, White D, Adams M, Young MJ, Kilian A, Zhang X, Shaffer HB, Unmack PJ (2018) Genomewide SNP markers breathe new life into phylogeography and species delimitation for the problematic short-necked turtles (Chelidae: Emydura) of eastern Australia. Molecular Ecology 27, 5195–5213.
| Genomewide SNP markers breathe new life into phylogeography and species delimitation for the problematic short-necked turtles (Chelidae: Emydura) of eastern Australia.Crossref | GoogleScholarGoogle Scholar |
Gruber B, Unmack PJ, Berry OF, Georges A (2018) DARTR: an R package to facilitate analysis of SNP data generated from reduced representation genome sequencing. Molecular Ecology Resources 18, 691–699.
| DARTR: an R package to facilitate analysis of SNP data generated from reduced representation genome sequencing.Crossref | GoogleScholarGoogle Scholar |
Guerin GR (2005) Nutlet morphology in Hemigenia R. Br. and Microcorys R. Br. (Lamiaceae). Plant Systematics and Evolution 254, 49–68.
| Nutlet morphology in Hemigenia R. Br. and Microcorys R. Br. (Lamiaceae).Crossref | GoogleScholarGoogle Scholar |
Haines ML, Stuart-Fox D, Sumner J, Clemann N, Chapple DG, Melville J (2017) A complex history of introgression and vicariance in a threatened montane skink (Pseudemoia cryodroma) across an Australian sky island system. Conservation Genetics 18, 939–950.
| A complex history of introgression and vicariance in a threatened montane skink (Pseudemoia cryodroma) across an Australian sky island system.Crossref | GoogleScholarGoogle Scholar |
Hmeljevski KV, Nazareno AG, Leandro Bueno M, dos Reis MS, Forzza RC (2017) Do plant populations on distinct inselbergs talk to each other? A case study of genetic connectivity of a bromeliad species in an Ocbil landscape. Ecology and Evolution 7, 4704–4716.
| Do plant populations on distinct inselbergs talk to each other? A case study of genetic connectivity of a bromeliad species in an Ocbil landscape.Crossref | GoogleScholarGoogle Scholar |
Hopper SD, Lambers H, Silveira FAO, Fiedler PL (2021) OCBIL theory examined: reassessing evolution, ecology and conservation in the world’s ancient, climatically buffered and infertile landscapes. Biological Journal of the Linnean Society 133, 266–296.
| OCBIL theory examined: reassessing evolution, ecology and conservation in the world’s ancient, climatically buffered and infertile landscapes.Crossref | GoogleScholarGoogle Scholar |
Hundsdoerfer AK, Lee KM, Kitching IJ, Mutanen M (2019) Genome-wide SNP data reveal an overestimation of species diversity in a group of hawkmoths. Genome Biology and Evolution 11, 2136–2150.
| Genome-wide SNP data reveal an overestimation of species diversity in a group of hawkmoths.Crossref | GoogleScholarGoogle Scholar |
Huson DH (1998) SplitsTree: analyzing and visualizing evolutionary data. Bioinformatics 14, 68–73.
| SplitsTree: analyzing and visualizing evolutionary data.Crossref | GoogleScholarGoogle Scholar |
Huson DH, Bryant D (2006) Application of phylogenetic networks in evolutionary studies. Molecular Biology and Evolution 23, 254–267.
| Application of phylogenetic networks in evolutionary studies.Crossref | GoogleScholarGoogle Scholar |
International Union for Conservation of Nature and Natural Resources (2022) Guidelines for Using the IUCN Red List Categories and Criteria. Version 15.1 (July 2022). Prepared by the Standards and Petitions Committee of the IUCN Species Survival Commission. Available at https://www.iucnredlist.org/resources/redlistguidelines [Verified 9 January 2023]
Joyce EM, Pannell CM, Rossetto M, Yap J-YS, Thiele KR, Wilson PD, Crayn DM (2021) Molecular phylogeography reveals two geographically and temporally separated floristic exchange tracks between Southeast Asia and northern Australia. Journal of Biogeography 48, 1213–1227.
| Molecular phylogeography reveals two geographically and temporally separated floristic exchange tracks between Southeast Asia and northern Australia.Crossref | GoogleScholarGoogle Scholar |
Jusaitis M (2018) Threatened plant translocation case study: Prostanthera eurybioides (Monarto Mintbush), Lamiaceae. Australasian Plant Conservation 26, 15–17.
Kilian A, Wenzl P, Huttner E, Carling J, Xia L, Blois H, Caig V, Heller-Uszynska K, Jaccoud D, Hopper C, Aschenbrenner-Kilian M, Evers M, Peng K, Cayla C, Hok P, Uszynski G (2012) Diversity arrays technology: a generic genome profiling technology on open platforms. Methods in Molecular Biology 888, 67–89.
| Diversity arrays technology: a generic genome profiling technology on open platforms.Crossref | GoogleScholarGoogle Scholar |
Koumoundouros T, Sumner J, Clemann N, Stuart-Fox D (2009) Current genetic isolation and fragmentation contrasts with historical connectivity in an alpine lizard (Cyclodomorphus praealtus) threatened by climate change. Biological Conservation 142, 992–1002.
| Current genetic isolation and fragmentation contrasts with historical connectivity in an alpine lizard (Cyclodomorphus praealtus) threatened by climate change.Crossref | GoogleScholarGoogle Scholar |
Mijangos JL, Gruber B, Berry O, Pacioni C, Georges A (2022) dartR v2: an accessible genetic analysis platform for conservation, ecology and agriculture. Methods in Ecology and Evolution 13, 2150–2158.
| dartR v2: an accessible genetic analysis platform for conservation, ecology and agriculture.Crossref | GoogleScholarGoogle Scholar |
Minchin PR (1987) An evaluation of the relative robustness of techniques for ecological ordination. Vegetatio 69, 89–107.
| An evaluation of the relative robustness of techniques for ecological ordination.Crossref | GoogleScholarGoogle Scholar |
Mitrovski P, Heinze DA, Broome L, Hoffmann AA, Weeks AR (2007) High levels of variation despite genetic fragmentation in populations of the endangered mountain pygmy-possum, Burramys parvus, in alpine Australia. Molecular Ecology 16, 75–87.
| High levels of variation despite genetic fragmentation in populations of the endangered mountain pygmy-possum, Burramys parvus, in alpine Australia.Crossref | GoogleScholarGoogle Scholar |
Montalvo AM, Conard SG, Conkle MT, Hodgskiss PD (1997) Population structure, genetic diversity, and clone formation in Quercus chrysolepis (Fagaceae). American Journal of Botany 84, 1553–1564.
| Population structure, genetic diversity, and clone formation in Quercus chrysolepis (Fagaceae).Crossref | GoogleScholarGoogle Scholar |
Narum SR, Buerkle CA, Davey JW, Miller MR, Hohenlohe PA (2013) Genotyping-by-sequencing in ecological and conservation genomics. Molecular Ecology 22, 2841–2847.
| Genotyping-by-sequencing in ecological and conservation genomics.Crossref | GoogleScholarGoogle Scholar |
NSW Department of Planning and Environment (2019) Saving our species Prostanthera gilesii 2018-2019 annual report card. Available at https://www.environment.nsw.gov.au/-/media/OEH/Corporate-Site/Documents/Animals-and-plants/Threatened-species/Report-cards/2018-2019/02-site-managed-flora/prostanthera-gilesii-2018-19.PDF
NSW National Parks and Wildlife Service (2009) Evans Crown Nature Reserve Plan of Management. DECC 2009/140. (NSW Department of Environment and Climate Change: Australia) Available at https://www.environment.nsw.gov.au/-/media/OEH/Corporate-Site/Documents/Parks-reserves-and-protected-areas/Parks-plans-of-management/evans-crown-nature-reserve-plan-of-management-090140.pdf [Verified 9 January 2023]
O’Donnell RP, Wilson TC, Andrew RL, Telford IR, Taseski GM, Zimmer H, Bruhl JJ (2021a) Molecular phylogenetic analysis of the Prostanthera phylicifolia (Lamiaceae) assemblage resolves relationships of the ‘Critically Endangered’ P. gilesii and other putative new species. Telopea 24, 359–375.
| Molecular phylogenetic analysis of the Prostanthera phylicifolia (Lamiaceae) assemblage resolves relationships of the ‘Critically Endangered’ P. gilesii and other putative new species.Crossref | GoogleScholarGoogle Scholar |
O’Donnell RP, Bruhl JJ, Telford IRH, Wilson TC, Zimmer HC, Taseski GM, Andrew RL (2021b) Molecular and morphological analyses support recognition of Prostanthera volucris (Lamiaceae), a new species from the Central Tablelands of New South Wales. bioRxiv 2021.12.21.473648
| Molecular and morphological analyses support recognition of Prostanthera volucris (Lamiaceae), a new species from the Central Tablelands of New South Wales.Crossref | GoogleScholarGoogle Scholar | [Published online 22 December 2021]
Osborne MJ, Norman JA, Christidis L, Murray ND (2000) Genetic distinctness of isolated populations of an endangered marsupial, the mountain pygmy-possum, Burramys parvus. Molecular Ecology 9, 609–613.
| Genetic distinctness of isolated populations of an endangered marsupial, the mountain pygmy-possum, Burramys parvus.Crossref | GoogleScholarGoogle Scholar |
Padial JM, Miralles A, De la Riva I, Vences M (2010) The integrative future of taxonomy. Frontiers in Zoology 7, 16
| The integrative future of taxonomy.Crossref | GoogleScholarGoogle Scholar |
Pembleton LW, Cogan NOI, Forster JW (2013) StAMPP: an R package for calculation of genetic differentiation and structure of mixed-ploidy level populations. Molecular Ecology Resources 13, 946–952.
| StAMPP: an R package for calculation of genetic differentiation and structure of mixed-ploidy level populations.Crossref | GoogleScholarGoogle Scholar |
Plunkett GT, Bruhl JJ, Telford IRH (2009) Two new, sympatric species of Wahlenbergia (Campanulaceae) from the New England Tableland escarpment, New South Wales, Australia. Australian Systematic Botany 22, 319–331.
| Two new, sympatric species of Wahlenbergia (Campanulaceae) from the New England Tableland escarpment, New South Wales, Australia.Crossref | GoogleScholarGoogle Scholar |
Robins TP, Binks RM, Byrne M, Hopper SD (2020) Contrasting patterns of population divergence on young and old landscapes in Banksia seminuda (Proteaceae), with evidence for recognition of subspecies. Biological Journal of the Linnean Society 133, 449–463.
| Contrasting patterns of population divergence on young and old landscapes in Banksia seminuda (Proteaceae), with evidence for recognition of subspecies.Crossref | GoogleScholarGoogle Scholar |
Sansaloni CP, Petroli CD, Carling J, Hudson CJ, Steane DA, Myburg AA, Grattapaglia D, Vaillancourt RE, Kilian A (2010) A high-density Diversity Arrays Technology (DArT) microarray for genome-wide genotyping in Eucalyptus. Plant Methods 6, 16
| A high-density Diversity Arrays Technology (DArT) microarray for genome-wide genotyping in Eucalyptus.Crossref | GoogleScholarGoogle Scholar |
Schlick-Steiner BC, Steiner FM, Seifert B, Stauffer C, Christian E, Crozier RH (2010) Integrative taxonomy: a multisource approach to exploring biodiversity. Annual Review of Entomology 55, 421–438.
| Integrative taxonomy: a multisource approach to exploring biodiversity.Crossref | GoogleScholarGoogle Scholar |
Scott J, Auld TD (2020) Conservation assessment of Prostanthera gilesii Althofer ex B.J.Conn & T.C.Wilson (family Lamiaceae). (NSW Department of Planning, Industry and Environment) Available at http://www.environment.gov.au/biodiversity/threatened/species/pubs/88743-conservation-advice-13112021.pdf
Searle SD, Bell JC, Moran GF (2000) Genetic diversity in natural populations of Acacia mearnsii. Australian Journal of Botany 48, 279–286.
| Genetic diversity in natural populations of Acacia mearnsii.Crossref | GoogleScholarGoogle Scholar |
Selwood KE, Zimmer HC (2020) Refuges for biodiversity conservation: a review of the evidence. Biological Conservation 245, 108502
| Refuges for biodiversity conservation: a review of the evidence.Crossref | GoogleScholarGoogle Scholar |
Silveira FAO, Fiedler PL, Hopper SD (2021) OCBIL theory: a new science for old ecosystems. Biological Journal of the Linnean Society 133, 251–265.
| OCBIL theory: a new science for old ecosystems.Crossref | GoogleScholarGoogle Scholar |
Slatyer RA, Nash MA, Miller AD, Endo Y, Umbers KD, Hoffmann AA (2014) Strong genetic structure corresponds to small-scale geographic breaks in the Australian alpine grasshopper Kosciuscola tristis. BMC Evolutionary Biology 14, 204
| Strong genetic structure corresponds to small-scale geographic breaks in the Australian alpine grasshopper Kosciuscola tristis.Crossref | GoogleScholarGoogle Scholar |
Smith JP, Heard TA, Beekman M, Gloag R (2017) Flight range of the Australian stingless bee Tetragonula carbonaria (Hymenoptera: Apidae). Austral Entomology 56, 50–53.
| Flight range of the Australian stingless bee Tetragonula carbonaria (Hymenoptera: Apidae).Crossref | GoogleScholarGoogle Scholar |
Sokal RR (1986) Phenetic Taxonomy: Theory and Methods. Annual Review of Ecology and Systematics 17, 423–442.
| Phenetic Taxonomy: Theory and Methods.Crossref | GoogleScholarGoogle Scholar |
Soltis DE, Gitzendanner MA, Stull G, Chester M, Chanderbali A, Chamala S, Jordon-Thaden I, Soltis PS, Schnable PS, Barbazuk WB (2013) The potential of genomics in plant systematics. Taxon 62, 886–898.
| The potential of genomics in plant systematics.Crossref | GoogleScholarGoogle Scholar |
Steane DA, Nicolle D, Sansaloni CP, Petroli CD, Carling J, Kilian A, Myburg AA, Grattapaglia D, Vaillancourt RE (2011) Population genetic analysis and phylogeny reconstruction in Eucalyptus (Myrtaceae) using high-throughput, genome-wide genotyping. Molecular Phylogenetics and Evolution 59, 206–224.
| Population genetic analysis and phylogeny reconstruction in Eucalyptus (Myrtaceae) using high-throughput, genome-wide genotyping.Crossref | GoogleScholarGoogle Scholar |
Sumner J, Haines ML, Lawrence P, Lawrence J, Clemann N (2021) Phylogenetic placement of a recently discovered population of the threatened alpine she-oak skink Cyclodomorphus praealtus (Squamata: Scincidae) in Victoria. Memoirs of Museum Victoria 153–157.
| Phylogenetic placement of a recently discovered population of the threatened alpine she-oak skink Cyclodomorphus praealtus (Squamata: Scincidae) in Victoria.Crossref | GoogleScholarGoogle Scholar |
Swofford D (2002) ‘PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods).’ (Sinauer Associates: Sunderland, MA, USA)
Symula R, Keogh JS, Cannatella DC (2008) Ancient phylogeographic divergence in southeastern Australia among populations of the widespread common froglet, Crinia signifera. Molecular Phylogenetics and Evolution 47, 569–580.
| Ancient phylogeographic divergence in southeastern Australia among populations of the widespread common froglet, Crinia signifera.Crossref | GoogleScholarGoogle Scholar |
Umbers KDL, Slatyer RA, Tatarnic NJ, Muschett GR, Wang S, Song H (2022) Phylogenetics of the skyhoppers (Kosciuscola) of the Australian Alps: evolutionary and conservation implications. Pacific Conservation Biology 28, 261–276.
| Phylogenetics of the skyhoppers (Kosciuscola) of the Australian Alps: evolutionary and conservation implications.Crossref | GoogleScholarGoogle Scholar |
van der Merwe MM, Yap J-YS, Wilson PD, Murphy HT, Ford A (2021) All populations matter: conservation genomics of Australia’s iconic purple wattle, Acacia purpureopetala. Diversity 13, 139
| All populations matter: conservation genomics of Australia’s iconic purple wattle, Acacia purpureopetala.Crossref | GoogleScholarGoogle Scholar |
von Mueller FJH (1858) Prostanthera phylicifolia. In ‘Fragmenta Phytographiae Australiae. Vol. 1’. p. 19. (Auctoritate Gubern: Melbourne, Vic., Australia)
Weir BS, Cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evolution 38, 1358–1370.
| Estimating F-statistics for the analysis of population structure.Crossref | GoogleScholarGoogle Scholar |
Williams ML, Drinnan AN, Walsh NG (2006) Variation within Prostanthera spinosa (Lamiaceae): evidence from morphological and molecular studies. Australian Systematic Botany 19, 467–477.
| Variation within Prostanthera spinosa (Lamiaceae): evidence from morphological and molecular studies.Crossref | GoogleScholarGoogle Scholar |
Wilson TC, Conn BJ (2015) Two new species of Prostanthera (Lamiaceae) from south-eastern Queensland. Telopea 18, 255–263.
| Two new species of Prostanthera (Lamiaceae) from south-eastern Queensland.Crossref | GoogleScholarGoogle Scholar |
Wilson TC, Conn BJ, Henwood MJ (2012) Molecular phylogeny and systematics of Prostanthera (Lamiaceae). Australian Systematic Botany 25, 341–352.
| Molecular phylogeny and systematics of Prostanthera (Lamiaceae).Crossref | GoogleScholarGoogle Scholar |
Wilson TC, Conn BJ, Henwood MJ (2017) Great expectations: correlations between pollinator assemblages and floral characters in Lamiaceae. International Journal of Plant Sciences 178, 170–187.
| Great expectations: correlations between pollinator assemblages and floral characters in Lamiaceae.Crossref | GoogleScholarGoogle Scholar |
Wilson TC, Carmen P, Hook C (2019) Recircumscription of Prostanthera denticulata R.Br. (Lamiaceae, Westringieae) and the new species P. crocodyloides T.C.Wilson. Telopea 22, 75–87.
| Recircumscription of Prostanthera denticulata R.Br. (Lamiaceae, Westringieae) and the new species P. crocodyloides T.C.Wilson.Crossref | GoogleScholarGoogle Scholar |
Wilson TC, Rossetto M, Bain D, Yap J-YS, Wilson PD, Stimpson ML, Weston PH, Croft L (2022) A turn in species conservation for hairpin banksias: demonstration of oversplitting leads to better management of diversity. American Journal of Botany 109, 1652–1671.
| A turn in species conservation for hairpin banksias: demonstration of oversplitting leads to better management of diversity.Crossref | GoogleScholarGoogle Scholar |
Worth JRP, Marthick JR, Jordan GJ, Vaillancourt RE (2011) Low but structured chloroplast diversity in Atherosperma moschatum (Atherospermataceae) suggests bottlenecks in response to the Pleistocene glacials. Annals of Botany 108, 1247–1256.
| Low but structured chloroplast diversity in Atherosperma moschatum (Atherospermataceae) suggests bottlenecks in response to the Pleistocene glacials.Crossref | GoogleScholarGoogle Scholar |
Xu S, Li L, Luo X, Chen M, Tang W, Zhan L, Dai Z, Lam TT, Guan Y, Yu G (2022) ggtree: a serialized data object for visualization of a phylogenetic tree and annotation data. iMeta 1, e56
| ggtree: a serialized data object for visualization of a phylogenetic tree and annotation data.Crossref | GoogleScholarGoogle Scholar |
Yap J-YS, Wilson TC, Rossetto M (2020) Conservation genomics of Prostanthera densa and P. marifolia: species status, management and translocation advice. November 2020 final report. (Research Centre for Ecosystem Resilience, Royal Botanic Garden: Sydney, NSW, Australia) Available at https://recer.org.au/wp-content/uploads/2022/09/Prostanthera-densa-and-P-marifolia-ReCER-Conservation-genomic-report.pdf
Yu G, Smith DK, Zhu H, Guan Y, Lam TT (2017) ggtree: an R package for visualization and annotation of phylogenetic trees with their covariates and other associated data. Methods in Ecology and Evolution 8, 28–36.
| ggtree: an R package for visualization and annotation of phylogenetic trees with their covariates and other associated data.Crossref | GoogleScholarGoogle Scholar |
