Maternal high-fat diet during pregnancy and lactation affects factors that regulate cell proliferation and apoptosis in the testis of adult progeny

Helen Viotti; Daniel Cavestany; Graeme B. Martin; Mark H. Vickers; Deborah M. Sloboda; Graciela Pedrana

doi:10.1071/RD23082

RESEARCH ARTICLE (Open Access)

Previous Contents Vol 36(9)

Maternal high-fat diet during pregnancy and lactation affects factors that regulate cell proliferation and apoptosis in the testis of adult progeny

Helen Viotti

^A , Daniel Cavestany

^A , Graeme B. Martin

^B ^* , Mark H. Vickers

^C , Deborah M. Sloboda

^D ^E ^F ^G and Graciela Pedrana

^A

+ Author Affiliations

- Author Affiliations

^A Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.

^B UWA School of Agriculture and Environment, and UWA Institute of Agriculture, University of Western Australia, Perth, WA, Australia.

^C Liggins Institute, University of Auckland, Auckland, New Zealand.

^D Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, Canada.

^E Department of Obstetrics and Gynecology, McMaster University, Hamilton, ON, Canada.

^F Department of Pediatrics, McMaster University, Hamilton, ON, Canada.

^G Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, ON, Canada.

^* Correspondence to: Graeme.Martin@uwa.edu.au

Handling Editor: Andrew Pask

Reproduction, Fertility and Development 36, RD23082 https://doi.org/10.1071/RD23082

Submitted: 8 May 2023 Accepted: 4 April 2024 Published online: 13 May 2024

© 2024 The Author(s) (or their employer(s)). Published by CSIRO Publishing. This is an open access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND)

Abstract

Context

A maternal high-fat diet is thought to pose a risk to spermatogenesis in the progeny.

Aims

We tested whether a maternal high-fat diet would affect Sertoli cell expression of transcription factors (insulin-like growth factor I (IGF-I); glial-cell line-derived neurotrophic factor (GDNF); Ets variant 5 (ETV5)) and cell proliferation and apoptotic proteins, in the testis of adult offspring.

Methods

Pregnant rats were fed ad libitum with a standard diet (Control) or a high-fat diet (HFat) throughout pregnancy and lactation. After weaning, male pups were fed the standard diet until postnatal day 160. Males were monitored daily from postnatal day 34 to determine onset of puberty. On postnatal day 160, their testes were processed for morphometry and immunohistochemistry.

Key results

The HFat diet increased seminiferous-tubule diameter (P < 0.03), the numbers of Sertoli cells (P < 0.0001) and Ki-67-positive spermatogonia (P < 0.0006), and the areas immunostained for ETV5 (P < 0.0001), caspase-3 (P < 0.001) and Bcl-2 (P < 0.0001). By contrast, the HFat diet reduced the areas immunostained for IGF-I (P < 0.01) and GDNF (P < 0.0001).

Conclusions

A maternal high-fat diet alters the balance between spermatogonia proliferation and spermatid apoptosis.

Implications

A maternal high-fat diet seems to ‘program’ adult male fertility.

Keywords: apoptosis, cell proliferation, developmental programming, high-fat diet, rat, reproduction, Sertoli cell, spermatogenesis, testis.

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