Analysis of the miRNA transcriptome during testicular development and spermatogenesis of the Mongolian horse
Bei Li
A , Xiaolong He B , Yiping Zhao A , Dongyi Bai A , Dandan Li A , Zhiyu Zhou A and Dugarjaviin Manglai A C
+ Author Affiliations
- Author Affiliations
A College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Scientific Observing and Experimental Station of Equine Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Equine Research Centre, Inner Mongolia Agricultural University, Zhaowuda RD.306, Hohhot, Inner Mongolia, PR China.
B Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Zhaojun RD.22, Hohhot, Inner Mongolia, PR China.
C Corresponding author. Email: dmanglai@163.com
Reproduction, Fertility and Development 32(6) 582-593 https://doi.org/10.1071/RD19133
Submitted: 13 August 2018 Accepted: 1 October 2019 Published: 6 February 2020
Abstract
Numerous studies have shown that microRNAs (miRNAs) are essential for testicular development and spermatogenesis. In order to further characterise these physiological processes, three immature and three mature testes of the Mongolian horse were collected and six libraries were established. Using small RNA sequencing technology, 531 mature miRNAs were identified, including 46 novel miRNAs without previously ascribed functions. Among the 531 miRNAs, 421 were expressed in both immature and mature libraries, 65 miRNAs were found solely in immature testis libraries and 45 miRNAs were found solely in mature testis libraries. Furthermore, among the miRNAs that were identified in both immature and mature libraries, 107 were significantly differentially expressed (corrected P value (padj) < 0.05). Among the miRNAs that were only expressed in immature testes, two miRNAs were differentially expressed, whereas among the miRNAs that were only expressed in mature testes, nine miRNAs were differentially expressed. Comprehensive analysis of miRNA and mRNA expression profiles predicted 107 miRNA–mRNA interaction sites. Gene ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis of the predicted target genes suggested roles of the differentially expressed miRNAs in testicular development and spermatogenesis. These findings identify miRNAs as key factors in the development of the testes and spermatogenesis in the Mongolian horse, which may also help us to understand the mechanisms of fertility in related mammalian species.
Additional keywords: equine, gametogenesis, non-coding RNA, reproductive development.
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