Analysis of the uterine flush fluid proteome of healthy mares and mares with endometritis or fibrotic endometrial degeneration
Mariana Diel de Amorim
A D F * , Firdous A. Khan A B E F * , Tracey S. Chenier A , Elizabeth L. Scholtz A and M. Anthony Hayes C
+ Author Affiliations
- Author Affiliations
A Department of Population Medicine, Ontario Veterinary College, University of Guelph, 50 Stone Road East, Guelph, ON, N1G2W1, Canada.
B Department of Large Animal Medicine and Surgery, School of Veterinary Medicine, St. George’s University, True Blue, St. George’s, Grenada.
C Department of Pathobiology, Ontario Veterinary College, University of Guelph, 50 Stone Road East, Guelph, ON, N1G2W1, Canada.
D Present address: Department of Clinical Sciences, Cornell University College of Veterinary Medicine, Ithaca, NY 14853, USA.
E Present address: Department of Large Animal Medicine and Surgery, School of Veterinary Medicine, St. George’s University, True Blue, St. George’s, Grenada.
F Corresponding authors. Emails: md649@cornell.edu; fkhan8@sgu.edu
Reproduction, Fertility and Development 32(6) 572-581 https://doi.org/10.1071/RD19085
Submitted: 5 March 2019 Accepted: 18 September 2019 Published: 28 January 2020
Abstract
The objective of this study was to evaluate the differences in the uterine flush fluid proteome between healthy mares and mares with endometritis or fibrotic endometrial degeneration (FED). Uterine flush fluid samples were collected from healthy mares (n = 8; oestrus n = 5 and dioestrus n = 3) and mares with endometritis (n = 23; oestrus n = 14 and dioestrus n = 9) or FED (n = 7; oestrus n = 6 and dioestrus n = 1). Proteomic analysis was performed using label-free liquid chromatography–tandem mass spectrometry. Of 216 proteins identified during oestrus, 127 were common to all three groups, one protein was exclusively detected in healthy mares, 47 proteins were exclusively detected in mares with endometritis and four proteins were exclusively detected in mares with FED. Of 188 proteins identified during dioestrus, 113 proteins were common between healthy mares and mares with endometritis, eight proteins were exclusively detected in healthy mares and 67 proteins were exclusively detected in mares with endometritis. Quantitative analysis revealed a subset of proteins differing in abundance between the three groups during oestrus and between healthy mares and mares with endometritis during dioestrus. These results provide a springboard for evaluation of specific proteins as biomarkers of uterine health and disease and for investigation of their roles in the establishment and maintenance of pregnancy.
Additional keywords: dioestrus, equine, fibrosis, inflammation, oestrus, proteomics, uterus.
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