In vitro production and cryotolerance of prepubertal and adult goat blastocysts obtained from oocytes collected by laparoscopic oocyte-pick-up (LOPU) after FSH treatment
Giovanni Giuseppe Leoni A D , Sara Succu A , Valentina Satta A , Mereu Paolo A , Luisa Bogliolo B , Daniela Bebbere C , Antonio Spezzigu C , Manuela Madeddu C , Fiammetta Berlinguer C , Sergio Ledda B and Salvatore Naitana C
+ Author Affiliations
- Author Affiliations
A Department of Physiological, Biochemical and Cellular Sciences, V. Vienna 2, 07100 Sassari, Italy.
B Department of Veterinary Pathology and Clinic, V. Vienna 2, 07100 Sassari, Italy.
C Department of Animal Biology, University of Sassari, V. Vienna 2, 07100 Sassari, Italy.
D Corresponding author. Email: gioleoni@uniss.it
Reproduction, Fertility and Development 21(7) 901-908 https://doi.org/10.1071/RD09015
Submitted: 20 January 2009 Accepted: 21 June 2009 Published: 27 July 2009
Abstract
This study compares the developmental capacity and cryotolerance of embryos produced from oocytes of stimulated prepubertal and adult Sarda goats. Twelve prepubertal and 13 adult goats were each given 110 and 175 IU FSH, respectively, and cumulus–oocyte complexes (COCs) were collected by laparoscopic oocyte-pick-up (LOPU). After in vitro maturation, fertilisation and culture (IVMFC), blastocysts were vitrified, warmed and blastocoel re-expansion and gene expression were evaluated. Prepubertal goats produced a higher COCs number than adults (mean ± s.e.m., 89.67 ± 5.74 and 26.69 ± 3.66, respectively; P < 0.01). Lower developmental competence was demonstrated in the prepubertal oocytes as shown by a higher number of COCs discarded before IVM (21.1% and 14.7% for prepubertals and adults, respectively; P < 0.01) and IVF (23.4% v. 9.1%; P < 0.01) and by the lower cleavage (55.6% and 70.3%, respectively; P < 0.01) and blastocyst rates (24.2% and 33.9%, respectively; P < 0.05). Compared with the adult, prepubertal vitrified/warmed blastocysts showed significantly (P < 0.05) lower in vitro viability, as determined by the re-expansion rate (62.5% and 40.3%). No differences were observed in the time required for blastocoel re-expansion or in cyclin B1, E-cadherin, Na/K ATPase, HSP90β and aquaporin 3 messenger RNA quantity. These results show that in vitro-produced embryos produced from prepubertal goat oocytes have a lower developmental rate and cryotolerance compared with their adult counterparts. However, we can assume that the quality of re-expanded embryos does not differ between the two groups.
Acknowledgements
This work was supported by RAS, special project P5A: Centro di Biodiversità animale. S. Succu received a grant from Fondazione Banco di Sardegna. We are grateful to Dr J. Kelly for his critical reading of the manuscript.
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