Hacking nature: genetic tools for reprograming enzymes
Carol J Hartley A , Matthew Wilding A and Colin Scott A B
+ Author Affiliations
- Author Affiliations
A CSIRO Land and Water
Black Mountain Research and Innovation Park
Canberra, ACT, Australia
B Tel: +61 2 6246 4090
Fax: +61 2 6246 4176
Email: colin.scott@csiro.au
Microbiology Australia 38(2) 73-75 https://doi.org/10.1071/MA17032
Published: 22 March 2017
Abstract
Enzymes have many modern industrial applications, from biomass decomposition in the production of biofuels to highly stereospecific biotransformations in pharmaceutical manufacture. The capacity to find or engineer enzymes with activities pertinent to specific applications has been essential for the growth of a multibillion dollar enzyme industry. Over the course of the past 50–60 years our capacity to address this issue has become increasingly sophisticated, supported by innumerable advances, from early discoveries such as the co-linearity of DNA and protein sequence1 to modern computational technologies for enzyme design. The design of enzyme function is an exciting nexus of fundamental biochemical understanding and applied engineering. Herein, we will cover some of the methods used in discovery and design, including some ‘next generation’ tools.
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