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Historical Records of Australian Science Historical Records of Australian Science Society
The history of science, pure and applied, in Australia, New Zealand and the southwest Pacific
RESEARCH ARTICLE (Open Access)

Hans Charles Freeman 1929–2008

Trevor W. Hambley A * and Ian D. Rae B
+ Author Affiliations
- Author Affiliations

A School of Chemistry, University of Sydney, NSW 2006, Australia.

B School of Chemistry, University of Melbourne, Vic. 3010, Australia.

* Correspondence to: trevor.hambley@sydney.edu.au

Historical Records of Australian Science 33(2) 180-190 https://doi.org/10.1071/HR21011
Published: 7 June 2022

© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing on behalf of the Australian Academy of Science. This is an open access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND)

Abstract

Hans Freeman was born in Germany and arrived in Australia with his parents in 1938. A brilliant student at the University of Sydney, he spent a seminal year at the California Institute of Technology before joining the staff at Sydney and initiating research on bioinorganic chemistry, studying metal ion complexes of compounds of biological significance such as amino acids, peptides and proteins. In his use of X-ray crystallography he was a pioneer in Australia, constructing his first crystallographic apparatus and mastering the necessary computing, at first by hand but soon with electronic computers. The culmination of his work with a series of collaborators was the structure of the blue, copper-containing metalloprotein, plastocyanin. Freeman also employed another advanced technique—X-ray spectroscopy and the study of X-ray absorption fine structure. He was a leading figure in Australia and internationally, and played an important role in gaining access for Australian scientists to international facilities such as synchrotron radiation sources at the dawning of the era of ‘Big Science’.

Keywords: bioinorganic chemistry, copper proteins, metal complexes, plastocyanin, synchrotron radiation, XAFS, X‐ray crystallography.


References

Anonymous (1973) Man of science for all seasons, Australian Women’s Weekly, 25 April, 2.

Anonymous (1990) Small Country—Big Science, Canberra. Available at https://nla.gov.au:443/tarkine/nla.obj-1745265283 [Viewed November 2020]

Anonymous (1992) Scientists to benefit from XAFS international short-course, Uniken, 6 November, 7. Available at https://nla.gov.au:443’tarkine/nla.obj-253834480 [Viewed November 2020]

Anonymous (2020) ‘History of the Australian synchrotron’. Available at http://archive.synchrotron.org.au/about‐us/history [Viewed November 2020]

Aroney, M. J., and Buckingham, A. D. (1988) Raymond James Wood Le Fevre 1905–1986, Historical Records of Australian Science, 7, 273–297.

Baker, A. T. (2021) David Mellor at the California Institute of Technology, 1937–8, the beginnings of Australian magnetochemistry, Historical Records of Australian Science, 32, 29–40.
David Mellor at the California Institute of Technology, 1937–8, the beginnings of Australian magnetochemistryCrossref | GoogleScholarGoogle Scholar |

Barber, J., and Halliwell, B. (1977) Photosynthesis at Reading, Nature, 270, 104–105.
Photosynthesis at ReadingCrossref | GoogleScholarGoogle Scholar |

Boldeman, J. W. (1997) ‘Access to major overseas research facilities’. Available at https://inis.iaea.org/collection/NCLCollectionStore/_Public/29/016/29016319.pdf?r==1&r==1 [viewed June 2021]

Branagan, D., and Holland, G. (eds) (1985) Ever Reaping Something New: a Science Centenary, Sydney.

Chapman, G. V., Colman, P. M., Freeman, H. C., Guss, J. M., Murata, M., Norris, V. A., Ramshaw, J. A., and Venkatappa, M. P. (1977) Preliminary crystallographic data for a copper-containing protein, plastocyanin, Journal of Molecular Biology, 110, 187–189.
Preliminary crystallographic data for a copper-containing protein, plastocyaninCrossref | GoogleScholarGoogle Scholar | 845945PubMed |

Colman, P. M., Freeman, H. C., Guss, J. M., Murata, M., Norris, V. A., Ramshaw, J. A., Venkatappa, M. P., and Vickery, L. E. (1977) Preliminary crystallographic data for a basic copper-containing protein from cucumber seedling, Journal of Molecular Biology, 112, 649–650.
Preliminary crystallographic data for a basic copper-containing protein from cucumber seedlingCrossref | GoogleScholarGoogle Scholar | 875035PubMed |

Colman, P. M., Freeman, H. C., Guss, J. M., Murata, M., Norris, V. A., Ramshaw, J. A., and Venkatappa, M. P. (1978a) ‘The structure of plastocyanin determined by x-ray diffraction at 2.7Å resolution’, in Photosynthesis 77—Proceedings of the Fourth International Congress on Photosynthesis, eds D. O. Hall, J. Coombs, T. W. Goodwin, London, pp. 810–813.

Colman, P. M., Freeman, H. C., Guss, J. M., Murata, M., Norris, V. A., Ramshaw, J. A. M., and Venkatappa, M. P. (1978b) X-ray crystal structure analysis of plastocyanin at 2.7 Å resolution, Nature, 272, 319–324.
X-ray crystal structure analysis of plastocyanin at 2.7 Å resolutionCrossref | GoogleScholarGoogle Scholar |

Creagh, D. (2018) ‘Personal profile’. Available at https://researchprofiles.canberra.edu.au/en/persons/dudley‐creagh[viewed November 2020]

Dickson, R. (1995) The golden oldies of inorganic chemistry, Australian Journal of Chemistry, 48, 689.
The golden oldies of inorganic chemistryCrossref | GoogleScholarGoogle Scholar |

Elkin, A. P. (1954) The Australian National Research Council, The Australian Journal of Science, 16, 203–211.

Fenner, F. (2005) The Australian Academy of Science: the First Fifty Years, Canberra.

Freeman, H. C. (1957) Crystallographic calculations on the silliac electronic digital computer. I. Fourier syntheses, Australian Journal of Chemistry, 10, 95–99.
Crystallographic calculations on the silliac electronic digital computer. I. Fourier synthesesCrossref | GoogleScholarGoogle Scholar |

Freeman, H. C. (1958) Crystallographic calculations on the silliac electronic digital computer: II: structure factors, Australian Journal of Chemistry, 11, 99–103.
Crystallographic calculations on the silliac electronic digital computer: II: structure factorsCrossref | GoogleScholarGoogle Scholar |

Freeman, H. C. (1967) Crystal structures of metal peptide complexes, Advances in Protein Chemistry, 22, 257–424.
Crystal structures of metal peptide complexesCrossref | GoogleScholarGoogle Scholar | 4882247PubMed |

Freeman, H. C. (1970) Proceedings of the XII International Conference on Coordination Chemistry, Sydney.

Freeman, H. C. (1973) ‘Metal complexes of amino acids and peptides’, in Inorganic Biochemistry, ed. G. L. Eichhorn,vol. 1, Amsterdam, pp. 121–166.

Freeman, H. C. (1981) Electron transfer in ‘blue’ copper proteins, Coordination Chemistry, 21, 29–51.

Freeman, H. C., and Guss, J. M. (2001a) ‘Plastocyanin’, in Handbook of Metalloproteins, ed. A. Messerschmidt, vol. 2, Boca Raton, pp. 1153–1169.

Freeman, H. C., and Guss, J. M. (2001b) ‘Cucumber basic protein’, in Handbook of Metalloproteins, ed. A. Messerschmidt, vol. 2, Boca Raton, pp. 1215–1218.

Freeman, H. C., and Smith, J. E. W. L. (1966) Crystallographic studies of the biuret reaction: II: structure of bis-biuret-copper(II) dichloride, Cu(NH2CONHCONH2)2Cl2, Acta Crystallographica, 20, 153–159.
Crystallographic studies of the biuret reaction: II: structure of bis-biuret-copper(II) dichloride, Cu(NH2CONHCONH2)2Cl2Crossref | GoogleScholarGoogle Scholar |

Freeman, H. C., Smith, J. E. W. L., and Taylor, J. C. (1959) Crystallographic studies of the biuret reaction, Nature, 184, 707–710.
Crystallographic studies of the biuret reactionCrossref | GoogleScholarGoogle Scholar | 13824733PubMed |

Freeman, H. C, Smith, J. E. W. L., and Taylor, J. C. (1961) Crystallographic studies of the biuret reaction: I. potassium bis-biuret cuprate(II) tetrahydrate, K2[Cu(NHCONHCONH)2].4H2O, Acta Crystallographica, 14, 407–418.
Crystallographic studies of the biuret reaction: I. potassium bis-biuret cuprate(II) tetrahydrate, K2[Cu(NHCONHCONH)2].4H2OCrossref | GoogleScholarGoogle Scholar |

Freeman, H. C., Norris, V. A., Ramshaw, J. A. M., and Wright, P. E. (1978) ‘High resolution proton magnetic resonance studies of plastocyanin’, in Photosynthesis 77—Proceedings of the fourth international congress on photosynthesis, eds. D. O. Hall, J. Coombs, T. W. Goodwin, London, pp. 805–809.

Freeman, H. C., Garrett, T. P J., Guss, J. M., Murata, M., Yoshizaki, F., Sugimura, Y., and Shimokoriyama, M. (1983) Preliminary crystallographic data for plastocyanins from an alga (Enteromorpha prolifera) and from cucumber (Cucumis sativus), Journal of Molecular Biology, 164, 351–353.
Preliminary crystallographic data for plastocyanins from an alga (Enteromorpha prolifera) and from cucumber (Cucumis sativus)Crossref | GoogleScholarGoogle Scholar | 6842595PubMed |

Garrett, R. F., Cookson, D. J., Foran, G., Creagh, D. C., and Wilkins, S. W. (1995) The Australian national beamline facility at the Photon Factory (abstract), Review of Scientific Instruments, 66, 1687–1687.
The Australian national beamline facility at the Photon Factory (abstract)Crossref | GoogleScholarGoogle Scholar |

Gilchrist, G. (1988) Peel me a cucumber, Hans, Sydney Morning Herald, Good Weekend, 10 December, 77.

Glusker, J. P. (1988) Edward Wesley Hughes 1904–1987, Journal of Applied Crystallography, 21, 283–284.
Edward Wesley Hughes 1904–1987Crossref | GoogleScholarGoogle Scholar |

Guss, J. M. (2012) Hans Charles Freeman (1929–2008): a scientific journey from dipole moments to protein crystallography, Journal of Inorganic Biochemistry, 115, 114–118.
Hans Charles Freeman (1929–2008): a scientific journey from dipole moments to protein crystallographyCrossref | GoogleScholarGoogle Scholar | 22554388PubMed |

Guss, J. M., and Freeman, H. C. (1983) Structure of oxidized poplar plastocyanin at 1.6 A resolution, Journal of Molecular Biology, 169, 521–563.
Structure of oxidized poplar plastocyanin at 1.6 A resolutionCrossref | GoogleScholarGoogle Scholar | 6620385PubMed |

Guss J. M., and Freeman, H. C. (2011a) Plastocyanin’, Encyclopedia of Inorganic and Bioinorganic Chemistry’, p. 10. Available at https://www.onlinelibrary.wiley.com/doi/10.1002/9781119951438.eibc0611 [viewed November 2020]

Guss, J. M., and Freeman, H. C. (2011b) ‘Cucumber basic protein’, Encyclopedia of Inorganic and Bioinorganic Chemistry, p. 10. Available at https://www.onlinelibrary.wiley.com/doi/10.1002/9781119951438.eibc0615 [viewed November 2020]

Guss, J. M., Harrowell, P. R., Murata, M., Norris, V. A., and Freeman, H. C. (1986) Crystal structure analyses of reduced (CuI) poplar plastocyanin at six pH value, Journal or Molecular Biology, 192, 361–387.
Crystal structure analyses of reduced (CuI) poplar plastocyanin at six pH valueCrossref | GoogleScholarGoogle Scholar | 3560221PubMed |

Guss, J. M., Merritt, E. A., Phizackerley, R. P., Hedman, B., Murata, M., Hodgson, K. O., and Freeman, H. C. (1988) Phase determination by multiple-wavelength x-ray diffraction: crystal structure of a basic “blue” copper protein from cucumbers, Science, 241, 806–811.
Phase determination by multiple-wavelength x-ray diffraction: crystal structure of a basic “blue” copper protein from cucumbersCrossref | GoogleScholarGoogle Scholar | 3406739PubMed |

Guss, J. M., Bartunik, H. D., and Freeman, H. C. (1992) Accuracy and precision in protein structure analysis: restrained least-squares refinement of the structure of poplar plastocyanin at 1.33 Å resolution, Acta Crystallographica, Section B: Structural Science, B48, 790–811.
Accuracy and precision in protein structure analysis: restrained least-squares refinement of the structure of poplar plastocyanin at 1.33 Å resolutionCrossref | GoogleScholarGoogle Scholar |

Guss, J. M., Merritt, E. A., Phizackerley, R. P., and Freeman, H. C. (1996) The structure of a phytocyanin, the basic blue protein from cucumber, refined at 1.8 Å resolution, Journal of Molecular Biology, 262, 686–705.
The structure of a phytocyanin, the basic blue protein from cucumber, refined at 1.8 Å resolutionCrossref | GoogleScholarGoogle Scholar | 8876647PubMed |

Hambley, T. (1995) Hans C. Freeman, Australian Journal of Chemistry, 45, 697–699.

Hambley, T. (2009) Hans Freeman 1929–2008, Journal of Biological Inorganic Chemistry, 14, 327–328.
Hans Freeman 1929–2008Crossref | GoogleScholarGoogle Scholar |

Hughes, E. W, Yakel, H. L., and Freeman, H. C. (1961) The crystal structure of biuret hydrate, Acta Crystallographica, 14, 345–352.
The crystal structure of biuret hydrateCrossref | GoogleScholarGoogle Scholar |

Hunter, R. J. (2017) ‘Boden, Alexander (1913–1993)’, Australian Dictionary of Biography. Available at http://adb.anu.edu.au/biography/boden-alexander-17438/text29161 [viewed November 2020]

Ji, J. (2006) ‘Celebrating 50 years of computing at Sydney’. Available at https://www.sydney.edu.au/news/84.hmtl?newsstoryid=1254 [viewed November 2020]

Kam, Z., Shore, H. B., and Feher, G. (1978) On the crystallization of proteins, Journal of Molecular Biology, 123, 539–555.
On the crystallization of proteinsCrossref | GoogleScholarGoogle Scholar | 691056PubMed |

Kendrew, J. C. (1954) Structure of proteins, Nature, 173, 57–59.
Structure of proteinsCrossref | GoogleScholarGoogle Scholar |

Lay, P. A., and Hambley, T. W. (2012) Hans C. Freeman preface, Journal of Inorganic Biochemistry, 115, 113.
Hans C. Freeman prefaceCrossref | GoogleScholarGoogle Scholar | 23026017PubMed |

Ludwig, M. L., and Lipscomb, W. N. (1973) ‘Carboxypeptidase A and other peptidases’, in Inorganic Biochemistry, ed. G. L. Eichhorn, vol. 1, Amsterdam, pp. 439–487.

Malmström, B. G. (1970) The ‘unique’ metal-binding properties of metalloenzymes, Pure and Applied Chemistry, 24, 393–406.
The ‘unique’ metal-binding properties of metalloenzymesCrossref | GoogleScholarGoogle Scholar |

Malmström, B. G., and Rosenberg, A. (1959) Mechanism of metal ion activation of enzymes, Advances in Enzymology—and Related Areas of Molecular Biology, 21, 131–167.

Newville, M. (2008) ‘Fundamentals of XAFS’. Available at https://www.lehigh.edu/imi/teched/GlassCSC/SuppReading/Tutorials.pdf [viewed November 2020]

Penfield, K. W., Gay, R. R., Himmelwright, R. S., Eickman, N. C., Norris, V. A., Freeman, H. C., and Solomon, E. I. (1981) Spectroscopic studies on plastocyanin single crystals: a detailed electronic structure determination of the blue copper active site, Journal of the American Chemical Society, 103, 4382–4388.
Spectroscopic studies on plastocyanin single crystals: a detailed electronic structure determination of the blue copper active siteCrossref | GoogleScholarGoogle Scholar |

Penner-Hahn, J. E., Murata, M., Hodgson, K. O., and Freeman, H. C. (1989) Low-temperature x-ray absorption spectroscopy of plastocyanin: evidence for copper-site photoreduction at cryogenic temperatures, Inorganic Chemistry, 28, 1826–1832.
Low-temperature x-ray absorption spectroscopy of plastocyanin: evidence for copper-site photoreduction at cryogenic temperaturesCrossref | GoogleScholarGoogle Scholar |

Rae, I. D. (2018) ‘Australian chemists crossing the Pacific to the promised land’, in Igniting the Chemical Ring of Fire, ed. S. C. Rasmussen, New Jersey, pp. 43–71.

Ross, I. (1996) Alexander Boden 1913–1993, Historical Records of Australian Science, 11, 523–540.
Alexander Boden 1913–1993Crossref | GoogleScholarGoogle Scholar |

Scopes, R. K. (1982) Protein Purification: Principles and Practice, 3rd edn, New York, pp. 335–342.

Scott, R. A., Hahn, J. E., Doniach, S., Freeman, H. C., and Hodgson, K. O. (1982) Polarized X-ray absorption spectra of oriented plastocyanin single crystals. Investigation of methionine-copper coordination, Journal of the American Chemical Society, 104, 5364–5369.

Stephens, T. (1958) From riches to rags to protein pioneer, The Sydney Morning Herald, 5 December 2009. Available at https://www.smh.com.au/from-riches-to-rags-to-protein-pioneer-20081205-gdt5d5.html [viewed October 2020]

Tullius, T. D., Frank, P., and Hodgson, K. O. (1978) Characterization of the blue copper site in oxidized azurin by extended x-ray absorption fine structure: determination of a short Cu─S distance, Proceedings of the National Academy of Sciences of the USA, 75, 4069–4073.
Characterization of the blue copper site in oxidized azurin by extended x-ray absorption fine structure: determination of a short Cu─S distanceCrossref | GoogleScholarGoogle Scholar | 16592557PubMed |

Vallee, B. L., and Williams, R. J. (1968) Metalloenzymes: the entatic nature of their active sites, Proceedings of the National Academy of Sciences USA, 59, 498–505.
Metalloenzymes: the entatic nature of their active sitesCrossref | GoogleScholarGoogle Scholar |

Ward, C. (2014) ‘Peter Malcolm Colman’. Available at https://csiropedia.csiro.au/colman-peter-malcolm [viewed November 2020]

Wilson, E. K. (2005) Keith Hodgson, Chemical & Engineering News, 83, 28.