Genetic variation for leaf carbon isotope discrimination and its association with transpiration efficiency in canola (Brassica napus)
Shek M. Hossain A D , Josette Masle B , Andrew Easton C E , Malcolm N. Hunter A , Ian D. Godwin A , Graham D. Farquhar B and Christopher J. Lambrides
A F
+ Author Affiliations
- Author Affiliations
A The University of Queensland, School of Agriculture and Food Sciences, Brisbane, Qld 4072, Australia.
B Plant Sciences, Research School of Biology, Australian National University, ACT 2601, Australia.
C Advanta Seeds Pty Ltd, 268 Anzac Avenue, Toowoomba, QLD 4350, Australia.
D Present address: CSIRO Agriculture and Food, Building 101, Clunies Ross Street, Black Mountain, ACT 2601, Australia.
E Present address: Natural Resource Management (NRM) North, PO Box 1224, Launceston, TAS 7250, Australia.
F Corresponding author. Email: chris.lambrides@uq.edu.au
Functional Plant Biology 47(4) 355-367 https://doi.org/10.1071/FP19256
Submitted: 30 August 2019 Accepted: 1 December 2019 Published: 5 March 2020
Abstract
Drought is a major constraint to canola production around the world. There is potential for improving crop performance in dry environments by selecting for transpiration efficiency (TE). In this work we investigated TE by studying its genetic association with carbon isotope discrimination (Δ) and other traits, e.g. specific leaf weight (SLW) and leaf chlorophyll content (SPAD). Among the 106 canola genotypes – including open-pollinated, hybrid, inbred types and cytoplasmic variants – tested in the field and glasshouse there was significant genotypic variation for TE, Δ, plant total dry weight, SLW and SPAD. Strong negative correlations were observed between TE and Δ (–0.52 to –0.76). Negative correlations between Δ and SLW or SPAD (–0.43 to –0.78) and smaller but significant positive correlations between TE and SLW or SPAD (0.23 to 0.30) suggested that photosynthetic capacity was, in part, underpinning the variation in TE. A cytoplasmic contribution to genetic variation in TE or Δ in canola was also observed with Triazine tolerant types having low TE and high Δ. This study showed that Δ has great potential for selecting canola germplasm with improved TE.
Additional keywords: C3 photosynthesis, drought tolerance.
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