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Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
Crop and Pasture Science

Crop and Pasture Science

Volume 73 Number 5 2022

Special Issue

Mineral Biofortification and Metal/Metalloid Accumulation in Food Crops (Part II)

Guest Editor:
Shahid Hussain

Foliar application of pesticides together with Zn fertiliser is a worthwhile and practical strategy to reduce application costs. A tank mix of zinc, thiamethoxam and propiconazole is safe and non-toxic to the crop, and the constituents are not antagonists to each other for pest management and biofortification. Due to cost effectiveness, farmers can make use of such combinations to control pests along with biofortification.


High-quality rice seeds produce uniform seed emergence in the direct seed sowing and a vigorous seedling rapidly improves seedling recovery after transplanting in the puddled field. A common problem for rice growers in the cultivation systems is low seed germination rates and seedling non-uniformity. Simultaneous priming of rice seed with Zn+K can be used to improve seed germination and seedling growth, whereas additional foliar Zn application increases grain yield and grain Zn concentration.

CP21194Seed priming with zinc sulfate and zinc chloride affects physio-biochemical traits, grain yield and biofortification of bread wheat (Triticum aestivum)

Abdul Rehman 0000-0002-4789-3462, Muhammad Farooq 0000-0003-4368-9357, Aman Ullah 0000-0002-5777-8310, Ahmad Nawaz, Muhammad Moeen ud Din and Babar Shahzad
pp. 449-460

Zinc (Zn) deficiency is major cause of micronutrient disorder in populations consuming cereal-based diets, particularly in developing countries where the high cost of chemical Zn fertilisers leads to low rates of soil and foliar Zn application. Seed priming with Zn is a cost-effective and ecofriendly approach which can enhance wheat productivity and grain biofortification. This study found that seeds of bread wheat may be primed with 0.5 M ZnSO4 as a Zn source for improving plant physiology, yield and grain biofortification.

CP21510Genotypic variability of grain phytic acid, mineral bioavailability, and their relation to foliar Zn application

Da Su 0000-0003-3597-0640, Muhammad Atif Muneer, Yuanyang Cai 0000-0003-1231-3111, Muhammad Abu Bakar Saddique and Fangmin Cheng
pp. 461-472

The article is bound to draw the attention of researchers and scientists towards the pigmented rice, the rich source of essential minerals including P, Zn, Fe, Mg, Cu, and Mn. This research gives an idea that pigmented rice should be used in rice-breeding programs to make rice an important functional food. In addition to that, the foliar spray of Zn on rice is an important management practice to boost grain Zn concentration, decrease phytic acid and increase mineral bioavailability. This is thought-giving research to rice breeders and agronomists on how they can make rice an excellent functional food for the global population.

CP21476Zinc sulfate application to grass forages (oat, barley, annual ryegrass and triticale) for increasing their yield, quality and profitability

Ahmad Sher 0000-0003-2320-0876, Sami Ul-Allah 0000-0003-1619-9432, Abdul Sattar, Muhammad Ijaz, Abdul Qayyum, Abdul Manaf and Muhammad Suleman
pp. 473-483

Zinc (Zn) deficiency is a global concern for human as well as animal health, but Zn fertilisation for productivity and biofortification of forages is not well studied. In our experiment, ZnSO4 fertilisation (15 kg ha−1) improved morphological traits, dry biomass yield, nutritional quality and straw Zn concentration of triticale, barley, oat and rye grass forage. In conclusion, a 15 kg ZnSO4 ha−1 fertilisation may be recommended to obtain higher fodder yield, forage quality and Zn biofortification in grass forages in semi-arid regions.

CP21438Exploring plant growth-promoting Streptomyces spp. for yield and nutrition traits in pearl millet hybrids

Vadlamudi Srinivas, Nimmala Naresh, Sambangi Pratyusha, Sravani Ankati, Mahalingam Govindaraj 0000-0003-0559-4015 and Subramaniam Gopalakrishnan
pp. 484-493

Biofortification of staple food crops with essential micronutrients has emerged as a sustainable solution to diminish malnutrition, especially in developing countries. This study showed PGP strains (Streptomyces) enhanced yield and nutritional traits (Fe, Zn) of pearl millet hybrids under greenhouse and field conditions. This study suggests the use of selected plant-growth promoters for enhancing stover and grain yield and its nutrient concentrations in pearl millet and can complement the existing conventional biofortification strategies.

CP21377Seed priming with boron and Bacillus sp. MN54 inoculation improves productivity and grain boron concentration of chickpea

Noman Mehboob, Waqas Ahmed Minhas, Muhammad Naeem, Tauqeer Ahmad Yasir, Muhammad Naveed, Shahid Farooq 0000-0002-6349-1404 and Mubshar Hussain 0000-0002-0696-6282
pp. 494-502

Boron (B) deficiency is the leading constraint to chickpea productivity in Pakistan. Results of this study elucidated that B seed priming at lower rates (0.001% and 0.0001%) along with seed inoculation with B-tolerant bacteria (Bacillus sp. MN54) improved the productivity and grain-B biofortification of chickpea. Higher levels of B seed priming (>0.1% B solution) proved toxic, with no germination recorded.

CP21415Seed-applied zinc-solubilising Bacillus biofertilisers improve antioxidant enzyme activities, crop productivity, and biofortification of maize

Muhammad Zahid Mumtaz 0000-0001-8014-7720, Maqshoof Ahmad 0000-0001-5438-5696, Muhammad Zafar-ul-Hye 0000-0001-6482-4656, Muhammad Saqib, Muhammad Fakhar U Zaman Akhtar and Muhammad Saqlain Zaheer
pp. 503-514

Plant growth-promoting bacteria are considered a possible promising biofortification intervention to address micronutrient malnutrition. Seed-applied zinc-solubilising Bacillus biofertiliser improved antioxidant enzyme activities and growth and yield attributes of maize, and biofortified maize grains under field conditions. Micronutrient biofortification in maize grains via biofertiliser application could be implemented to overcome malnutrition issues in regions with cereal-based staple diets.

CP21493Effects of environments and cultivars on grain ionome of spring wheat grown in Kazakhstan and Russia

Alexey Morgounov 0000-0001-7082-5655, Timur Savin, Paulina Flis, Adylkhan Babkenov, Vladimir Chudinov, Anastasiya Kazak, Hamit Koksel, Ivan Likhenko, Ram Sharma, Tatyana Shelaeva, Sergey Shepelev, Ekaterina Shreyder and Vladimir Shamanin
pp. 515-527

Spring wheat grain ionomics analysis was conducted to evaluate the relative contributions of environment and genotype to variation in elemental composition, based on data from six sites in Kazakhstan and Russia in 2017–18. For concentrations of P, S, Cu, Mn and Mo, the effect of site was 2–3 times higher than the effect of genotype, whereas for Ca, Mg, Fe, Cd and Sr, genotype and site effects were similar. A combination of high grain yield, relatively high protein content, and high concentration of P, S, Mn, Cu and/or Zn was identified in three genotypes.

CP21160Quantifying the required Zn uptake to achieve grain Zn biofortification of high-yielding wheat on calcareous soils with low available Zn

Sen Wang 0000-0001-9793-4472, Zhaohui Wang, Shasha Li, Chaopeng Diao, Lu Liu, Ning Huang, Ming Huang, Xiaoli Hui, Laichao Luo, Gang He and Hanbing Cao
pp. 528-536

An effective solution to address global human Zn deficiency is to increase Zn concentration and grain yield of staple food crops, but this has rarely been achieved in many wheat production regions. This study quantified the adequate Zn uptake or distribution to achieve high grain Zn concentration (∼40 mg kg−1) and high yield (∼7 t ha−1) for bread wheat. The findings can provide quantitative guidelines for improving wheat Zn nutrition in different environments.

CP21267Accumulation of zinc, iron and selenium in wheat as affected by phosphorus supply in salinised condition

De-Yong Zhao 0000-0002-1336-1150, Zai-Wang Zhang, Yu-Rong Yuan, Xiao-Lin Zhang, Wang-Feng Zhao, Xue-Ping Li, Jun Wang and Kadambot H. M. Siddique 0000-0001-6097-4235
pp. 537-545

This paper deals with the effects of phosphorus (P) supply and salinity on zinc (Zn), iron (Fe) and selenium (Se) accumulation in wheat, as well as the genotypic difference in Zn, Fe and Se accumulation among genotypes. Significant interactive effects between P supply and salinity were detected on Zn, Fe and Se accumulation, suggesting that P supply is a key factor regulating biomass and accumulation of Zn, Fe and Se in certain saline soils. Substantial genotypic differences among genotypes provided potential scope for genetic improvement.

CP21478Resistance to NaCl salinity is positively correlated with iron and zinc uptake potential of wheat genotypes

Ghulam Abbas 0000-0003-1074-7530, Sadia Rehman, Muhmmad Saqib, Muhammad Amjad, Behzad Murtaza, Manzer H. Siddiqui and Yinglong Chen 0000-0003-0798-8683
pp. 546-555

Soil salinity is a serious environmental issue that is reducing crop productivity and the uptake of important micronutrients including iron and zinc in different plants. The present work identified the wheat genotypes with better iron and zinc uptake potential under saline and nutrient limited conditions. The results are very promising for farmers to select best wheat genotypes for saline soils prune to nutrient deficiency.


This study investigated the potential of Si and Zn enriched miscanthus biochar to reduce the toxic impacts of NaCl stress on early growth stage of radish crop. Si-En-Bc proved to be the best treatment while Zn-En-Bc not only compensate NaCl stress but also enhance Zn availability. Overall, Si and Zn enriched miscanthus biochar could be an excellent addition to the available organic amendments against NaCl stress for increasing soil fertility and improving plant nutritional and defensive mechanisms.

CP21437Appraisal of functional significance of sulfur assimilatory products in plants under elevated metal accumulation

Bilal A. Rather, Iqbal R. Mir 0000-0003-3435-0458, Harsha Gautam, Arif Majid, Naser A. Anjum, Asim Masood and Nafees A. Khan 0000-0001-7957-5832
pp. 573-584

Heavy-metal accumulation in plants is inevitable. Plant sustenance under elevated cellular heavy metals is decided by their inherent capacity of the potential in synthesising metal-chelating and/or -detoxifying compounds. Sulfur (S) is the most important element that is incorporated into cysteine (Cys), which acts as a precursor for several reduced S compounds. Thus, S contributes to resistance of plants to heavy metals.

CP21240Risk assessment of using phosphate and calcium fertilisers for continuously flooded rice cultivation in a soil co-contaminated with cadmium and antimony

ShengJie Shi 0000-0001-5283-1816, QianHua Wu, YanMing Zhu, ZhiLian Fan, Christopher Rensing, Hong Liu and RenWei Feng
pp. 585-598

Flooding conditions will result in excess accumulation of arsenic (As) in the grains of rice plants. NaH2PO4 generally enhanced the soil release of As and Sb by increasing pH and competitive adsorption in the soil, which resulted in their accumulation in many tissues of the rice plants. The treatment of CaCl2 at 200 mg kg−1 was most effective for reducing As, Sb and Cd concentrations in the grains.

CP21329Role of antioxidative defense system in amelioration of cadmium-induced phytotoxic effects in germinating seeds of maize (Zea mays)

Aamer Abbas, Muhammad Sajid Aqeel Ahmad 0000-0002-7310-2898, Muhammad Ashraf, Qasim Ali 0000-0002-3773-5196 and Ambreen Khadija Alvi
pp. 599-613

As compared with other biologically essential metals (Mn, Cu and Zn), Cd is not considered essential because its biological function is not reported in literature. It has a wide range of toxic effects on seed germination and other physiological and biochemical processes. In this study, the role of enzymatic antioxidative enzymes, malondialdehyde (MDA), ascorbic acid (AsA) and total phenols in ameliorating phytotoxic effects of cadmium was upraised in two most widely cultivated maize genotypes.

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