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Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
Table of Contents
Crop and Pasture Science

Crop and Pasture Science

Volume 73 Numbers 1 & 2 2022

Special Issue

Mineral Biofortification and Metal/Metalloid Accumulation in Food Crops

Guest Editor:
Shahid Hussain

CP20455Biofortification of wheat with zinc as affected by foliar applications of zinc, pesticides, phosphorus and biostimulants

Peng Ning, Xiaoyuan Zhang, Tianqi Wu, Yafei Li, Shaoxia Wang, Peiwen Fei, Jinjin Dong, Jianglan Shi and Xiaohong Tian 0000-0002-7594-9726
pp. 3-12

Incorporating foliar zinc (Zn) application into common management, e.g. foliar application of pesticides, KH2PO4 or biostimulants, is a feasible agronomic strategy to biofortify wheat with Zn. However, in this study, both environmental conditions and foliar Zn management, e.g. Zn forms and spray regimes (alone or combined) contributed to the magnitude of grain Zn enrichment, which accounted for 20–37% and 35–45% of explained variation, respectively. The study highlights that management and environment interactions need to be considered in agronomic biofortification of food crops with micronutrients.

CP21129Effect of Zn and sucrose supply on grain Zn, Fe and protein contents within wheat spike under detached-ear culture

Yinghua Zhang 0000-0002-1356-6084, Na Liu, Naiyue Hu, Wanqing Zhang, Zhencai Sun and Zhimin Wang
pp. 13-21

This study clarified the effect of grain position, and sucrose and Zn supply level on grain weight, and Zn, Fe and protein contents under detached-ear culture. Results showed that the central spikelets had a higher grain weight and nutrient content than did basal and apical spikelets; increasing Zn and sucrose supply improved grain weight and grain Zn, Fe and protein content, and the increasing percentage was higher in the central spikelet than in apical/basal spikelet. This study has provided a reference for manipulating grain weight and grain Zn, Fe and protein content.


Plant growth relies on the bioavailability of essential nutrients in their growth environment. Access to mineral nutrient is challenging for plants grown in alkaline soil, and in these environments, the postharvest application may be effective. Mineral fortification of harvested tomatoes by ZnO nanoparticles enhanced their Zn content by 17%.

CP21140Enhancing the accumulation and bioavailability of iron in rice grains via agronomic interventions

Usman Zulfiqar, Saddam Hussain 0000-0002-0895-1287, Muhammad Maqsood, Shahid Ibni Zamir, Muhammad Ishfaq, Nauman Ali, Muhammad Ahmad and Muhammad Faisal Maqsood
pp. 32-43

Malnutrition of iron (Fe) in cereals occurs worldwide, particularly, Fe deficiency is more apparent in food crops grown on high-pH soils. Agronomic biofortification seems a feasible solution; however, accumulation and bioavailability of Fe may vary with application method and crop management regime. The presented study, for the first time, reports the comparative efficacy of various Fe application methods (seed coating, osmopriming, surface broadcasting, foliar application) on paddy yield, net benefits, grain Fe accumulation, bioavailability, and Fe-use efficiencies in conventional (puddled transplanted rice) and conservational (direct-seeded aerobic rice) production systems.

CP20519Selenium application influenced selenium biofortification and physiological traits in water-deficit common bean plants

Ruby Antonieta Vega Ravello 0000-0002-7185-5677, Cynthia de Oliveira 0000-0002-8030-4025, Josimar Lessa 0000-0002-2739-3436, Lissa Vasconcellos Vilas Boas 0000-0003-3883-5495, Evaristo Mauro de Castro 0000-0002-1385-8503, Luiz Roberto Guimarães Guilherme 0000-0002-5387-6028 and Guilherme Lopes 0000-0002-7898-798X
pp. 44-55

Agronomic biofortification improves the nutritional value of plant-based foods and, therefore, aids human nutrition. Biofortification for producing selenium-rich common bean grains added selenium for humans and activated the plant’s defence system to face water stress that they were exposed to. Biofortification with selenium enriches crops and it is a valuable agricultural tool to improve food security and face climate change.

CP21059Selenium desorption in tropical soils by sulfate and phosphate, and selenium biofortification of Mombaça grass under increasing rates of phosphate fertilisation

Maria Jéssica Vieira dos Santos, Josimar Henrique de Lima Lessa, Mateus Belisário de Assis, Jéssica Francisco Raymundo, Bruno Teixeira Ribeiro, Luiz Roberto Guimarães Guilherme and Guilherme Lopes 0000-0002-7898-798X
pp. 56-66

Selenium availability in soils is influenced by phosphate and sulfate. This study evaluated Se desorption by these anions and Se biofortification of grass under phosphate fertilisation rates, showing that Se desorption upon increasing S or P rate depends on soil attributes and that Se desorbed from soils and absorbed by the grass increased with increasing P rate. These findings are useful because P and S additions are soil management practices that also may be used to increase soil Se availability.


This study examined the growth characteristics, chlorophyll content, and nutritional properties of Tetragonia decumbens cultivated in hydroponics and reported macronutrients and micronutrients present in T. decumbens. Findings also showed that nutrient applications did not affect micronutrients; however, macronutrients were affected. The study suggests that a well-drained soilless media (100% silica) with high nutrient application, low electrical conductivities, and moderate pH levels are best for vegetative growth and nutritional characteristics of T. decumbens.

CP20498Next-generation technologies for iron and zinc biofortification and bioavailability in cereal grains

S. Ibrahim, B. Saleem 0000-0002-3037-7998, M. K. Naeem, S. M. Arain and M. R. Khan
pp. 77-92

Iron and zinc are among the key micronutrients essential for human health and normal growth of body. Conventional breeding approaches are laborious and time-consuming compared with advanced breeding approaches such as CRISPR-Cas9, and have had little success with Fe biofortification. Precise editing by CRISPR-Cas9 will help to enhance the Fe and Zn content in cereals without any linkage-drag and biosafety issues.

CP21116Grain micronutrient evaluation of wheat (Triticum aestivum) germplasm and molecular characterisation via genic and random SSR markers

Mohd. Tahir, Safoora Shafi, Mohd. Anwar Khan, Farooq Ahmad Sheikh, Mohd. Ashraf Bhat, Parvaze Ahmad Sofi, Satish Kumar, Mohd. Altaf Wani and Reyazul Rouf Mir 0000-0002-3196-211X
pp. 93-103

Micronutrient malnutrition is a global; therefore, in the present study we evaluated advanced breeding lines of wheat for grain Zn and Fe concentrations for selection of the most promising genotypes. Genetic diversity and genes for Zn and Fe concentrations were identified for wheat molecular breeding programs. The promising genotypes and useful genes identified for Zn and Fe concentrations will prove useful in alleviating micronutrient malnutrition.

CP21104Genetic and molecular characterisation of subtropically adapted low-phytate genotypes for utilisation in mineral biofortification of maize (Zea mays)

Shridhar Ragi, Vignesh Muthusamy 0000-0003-3169-890X, Rajkumar U. Zunjare, Vinay Bhatt, Ashvinkumar Katral, Krishnan P. Abhijith, Ravindra Kasana, Nisrita Gain, Javaji C. Sekhar, Devendra K. Yadava and Firoz Hossain 0000-0001-6662-7752
pp. 104-115

Phytic acid is a major anti-nutritional factor in maize grains that significantly reduces the bioavailability of minerals such as iron and zinc in humans, leading to micronutrient malnutrition. We developed and evaluated genetically diverse maize inbreds with lpa1-1 gene that possess low phytic acid, good agronomic performance and grain yield. These inbreds would offer great potential in development of low-phytate maize hybrids with highly bioavailable minerals in the maize-based diet, to alleviate micronutrient deficiencies in humans.

CP21206Relationship of grain micronutrient concentrations and grain yield components in a doubled haploid bread wheat (Triticum aestivum) population

Mantshiuwa C. Lephuthing 0000-0001-6356-7686, Vicki L. Tolmay 0000-0002-7374-5295, Timmy A. Baloyi, Tsepiso Hlongoane, Tebogo A. Oliphant and Toi J. Tsilo 0000-0001-6987-8573
pp. 116-126

We studied the relationship between grain micronutrient content and grain yield components, and their associated structures in doubled haploid bread wheat lines. A wide range of phenotypic variation was observed for all traits. Best performing doubled haploid lines showing high values for both grain iron and zinc concentrations, together with high values for one or more grain yield components, were identified.


In terms of biofortification strategies, spring wheat Almaken and Zhenis M5 mutant lines produced via irradiation with 200 Gy gamma and parents were analysed for the expression of genes involved in iron homeostasis under normal and iron-deficient levels of growth conditions. Genotype-dependent and tissue-specific gene expression differences in response to iron deficiency were revealed that provide new insights into Fe translocation, storage, and regulation in wheat.


Legumes are one of the largest and most cultivated crops in the world. Unfortunately, Fe deficiency is among the important abiotic constraints that reduce legumes productivity around the world. The results of the present study revealed the importance of the efficiency of antioxidant system in barrel medic (plant model of legumes) to cope with Fe deficiency.

CP21033Sustainable solutions to arsenic accumulation in rice grown in south and south-east Asia

Sudhakar Srivastava 0000-0001-6943-8367, Saurabh Pathak, Montree Ponsin, Supanad Hensawang, Penradee Chanpiwat, Chetra Yoeurn and Kongkea Phan
pp. 149-159

In this review article the arsenic problem in rice in south and south-east Asia and possible mitigation techniques are discussed. During cultivation, agronomic sustainable strategies like water management, soil amendment, fertiliser amendment selection of rice cultivar for arsenic reduction in rice are presented. Post harvesting, processing techniques, and cooking methods to achieve arsenic reduction at personal level are discussed. The socioeconomic aspects of the arsenic problem are also reviewed.

CP21157Cadmium and arsenic provoke mostly distinct but partly overlapping responses in Brassica juncea

Allah Dad Khan, Muhammad Sayyar Khan 0000-0003-0579-1772, Sajid Ali Khan Bangash, Kashif Naeem, Abdullah Jalal and Muhammad Tayyab
pp. 160-169

Cadmium and arsenic contamination of agricultural lands poses a serious threat to the environment and crop productivity. Although both are detoxified via a common detoxification pathway, we found that they mostly trigger distinct physiological and biochemical responses in Brassica juncea plants. The data are valuable for design of strategies for sustainable growth of plants on cadmium and arsenic polluted soils.

CP21080Chitosan-modified biochar immobilised arsenic in root medium and enhanced the growth of zucchini (cv. Courgette d’Italie) seedlings

Sajid Mehmood 0000-0002-6936-4975, Waqas Ahmed, Muhammad Imtiaz, Muhammad Qaswar, Muhammad Ikram, Saqib Bashir 0000-0001-8573-6591, Muhammad Rizwan, Sana Irshad, Shuxin Tu, Weidong Li and Di-Yun Chen
pp. 170-180

Contamination of arsenic (As) presents a health hazard that affects home gardeners neighbouring contaminated environments. Chitosan modified biochar (BR-C) and as-is biochars are vital due to their role in removing As(V) from an aqueous environment using zucchini as a test crop. Results showed that BR-C, can increase the sorption capacity of As from polluted water and resist the uptake of As form the crop.

CP21322Plant–microbe–metal interactions for heavy metal bioremediation: a review

Md. Saiful Islam, Tapos Kormoker 0000-0001-7552-5627, Abubakr M. Idris 0000-0003-4038-4769, Ram Proshad, Md. Humayun Kabir and Fikret Ustaoglu
pp. 181-201

This review highlights the biological remediation technique (i.e. organic residues–plant–microbes) for bioremediation to the natural environment, and seeks an appropriate method for investigating the biosorption, bioaccumulation and bioavailability of heavy metals in soil before selecting a remediation technology for metal remediation from soil. The review finds that plant-microbe-metals interactions and microbial-assisted phytoremediation are essential for heavy metals remediation from contaminated soils.

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