Identification of endosperm and maternal plant QTLs for protein and lysine contents of rice across different environments
C. H. Shi A C , Y. Shi A , X. Y. Lou B , H. M. Xu A , X. Zheng A and J. G. Wu A CA Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, P. R. China.
B Department of Psychiatry and Neurobehavioral Sciences, University of Virginia, Charlottesville, VA 22911, U.S.A.
C Corresponding authors. Emails: chhshi@zju.edu.cn; jgwu@zju.edu.cn
Crop and Pasture Science 60(3) 295-301 https://doi.org/10.1071/CP08234
Submitted: 15 July 2008 Accepted: 16 December 2008 Published: 16 March 2009
Abstract
Using a newly developed mapping model with endosperm and maternal main effects and QTL × environment interaction effects on quantitative quality traits of seed in cereal crops, the investigation of quantitative trait loci (QTLs) located on triploid endosperm and diploid maternal plant genomes for protein content and lysine content of rice grain under different environments was carried out with two backcross (BC1F1 and BC2F1) populations from a set of 241 recombinant inbred lines derived from an elite hybrid cross of Shanyou 63. The results showed a total of 18 QTLs to be associated with these two quality traits of rice, which were subsequently mapped on chromosomes 2, 3, 5, 6, 7, 10, 11 and 12. Three of these QTLs were also found having QTL × environment interaction effects. Therefore, the genetic main effects from QTLs located on chromosomes in endosperm and maternal plant genomes and their QTL × environment interaction effects in different environments were all important for protein and lysine contents in rice. The influence of environmental factors on the expression of some QTLs located in different genetic systems could not be ignored for both nutrient quality traits.
Additional keywords: nutrient quality trait of rice, quantitative trait locus (QTL), genetic main effect, QTL × environment interaction effect.
Acknowledgments
The project is supported by the National Natural Science Foundation of China (no. 30571198), the National High Technology Research and Development Program of China (2006AA100101), the Science and Technology Office of Zhejiang Province (nos. 011102471 and 2007C22016), and the 151 Foundation for the Talents of Zhejiang Province. We are also greatly indebted to Prof. Q. F. Zhang for kindly providing materials and molecular data.
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