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RESEARCH ARTICLE
Contents Vol 59(12)

Histopathology comparison and phenylalanine ammonia lyase (PAL) gene expressions in Fusarium wilt infected watermelons

P.-F. L. Chang A C , C.-C. Hsu A , Y.-H. Lin A , K.-S. Chen B , J.-W. Huang A and T.-D. Liou B
+ Author Affiliations
- Author Affiliations

A Department of Plant Pathology, National Chung Hsing University, Taichung, Taiwan 402, R.O.C.

B Fengshan Tropical Horticultural Experiment Branch, Agricultural Research Institute, Council of Agriculture, Fengshan, Kaohsiung, Taiwan 830, R.O.C.

C Corresponding author. Email: pfchang@nchu.edu.tw

Australian Journal of Agricultural Research 59(12) 1146-1155 https://doi.org/10.1071/AR08066
Submitted: 20 February 2008  Accepted: 7 October 2008   Published: 10 November 2008

Abstract

Fusarium wilt disease of watermelon (Citrullus lanatus (Thunb.) Matsum & Nakai), caused by Fusarium oxysporum f. sp. niveum (FON), is one of the limiting factors of worldwide watermelon production. In this study, a Fusarium wilt resistant watermelon JSB, which was derived from a spontaneous mutation of the susceptible Sugar Baby (SB), was used to investigate histopathology. The number and diameter of xylem vessels in the root (10 mm below the shoot base) of resistant JSB plants were significantly higher than those in susceptible SB plants. At 9 days post inoculation (dpi), using the plate assay on Nash-PCNB media, FON could be recovered from 86% of the roots in the symptomless plants of both watermelon lines, and from 55% and 64% of the stem segments (5 mm above the shoot base) in resistant and susceptible plants, respectively. In paraffin and free-hand tissue sections, at 8, 13, and 35 dpi, the xylem of roots and stems close to the soil surface in resistant watermelon JSB plants was also colonised by FON, but to a much lower percentage than the susceptible SB ones. No colonisation below the middle of stems was observed in the resistant JSB plants. The susceptible plants grown in infested soil were all dead by 35 dpi, while the resistant plants remained healthy. These observations suggest that reducing FON colonisation in the vascular systems of the host may contribute to the resistance in JSB. Furthermore, the higher expression of the phenylalanine ammonia lyase (PAL) gene in JSB induced by FON and the effects of PAL inhibitor on the resistance of JSB suggested that PAL is involved in resistance of watermelon to Fusarium wilt pathogen.

Additional keywords: watermelon, Fusarium oxysporum f. sp. niveum, resistance mechanism, xylem, plant histopathology.


Acknowledgments

The authors thank Dr S.-J. Hsiao (National Chung Hsing University, Taiwan) for her technical support in paraffin cross- and free-hand sectioning. We are grateful to Drs W. H. Ko (National Chung Hsing University, Taiwan) and Gad Loebenstein (The Agricultural Research Organization of Israel) for critical reading and suggestions on this manuscript. This research was supported in part by grants: 90AS-2.1.2-BQ-B7, NSC 91-2313-B-005-094, NSC 92-2313-B-005-054, and NSC 93-2313-B-005-080; by the Ministry of Education, Taiwan, R.O.C., under the ATU plan; and by the National Chung Hsing University.


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