Genetics of grain dormancy in a white wheat
M.-K. Tan A D E , P. J. Sharp B D , M.-Q. Lu C and N. Howes B DA NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Woodbridge Rd, PMB 8, Camden, NSW 2570, Australia.
B Plant Breeding Institute, University of Sydney, PMB 11, Camden, NSW 2570, Australia.
C Australian Grain Technologies, PO Box 219, Narrabri, NSW 2390, Australia.
D Value Added Wheat CRC Ltd, Locked Bag 1345, PO North Ryde, NSW 2113, Australia.
E Corresponding author. Email: mui-keng.tan@dpi.nsw.gov.au
Australian Journal of Agricultural Research 57(11) 1157-1165 https://doi.org/10.1071/AR06101
Submitted: 28 March 2006 Accepted: 24 July 2006 Published: 27 October 2006
Abstract
A white-grained wheat accession, AUS1408, is a current major source of pre-harvest sprouting (PHS) tolerance in Australian breeding programs. This study has located 2 significant quantitative trait loci (QTLs) for its grain dormancy on 4AL and 5BL. Their associations with seed dormancy have been determined from population-level marker-trait associations (with 3 years of phenotype data) and confirmed by transmission/disequilibrium test on selected advanced breeding lines. The 4AL QTL was expressed in all years of testing, with phenotypic variance ranging from 5 to 15%, indicating a strong genotype × environment interaction. This QTL has been reported in wheat cultivars of diverse origin and was also found to be strongly influenced by the environment. The 5BL QTL was found to have a remarkably consistent effect on the trait at a phenotypic variance of around 10%. The successful outcome in this study was facilitated by high throughput DArT mapping, which complemented mapping with microsatellite markers for critical QTL identification. Identification of these QTLs from AUS1408 should enable sprouting tolerance derived from this source to be incorporated into advanced breeding lines, with the use of molecular markers reducing the requirement for multi-year field testing.
Additional keywords: pre-harvest sprouting tolerance, DarT, transmission/disequilibrium test, blackpoint.
Acknowledgments
We gratefully acknowledged the careful technical assistance of R. Srivastava and R. Li in the laboratory processing of samples, and of Graham Johnson in photography. Grateful thanks are expressed to Dr D. Mares (University of Adelaide) for characterisation of the grain dormancy, and to Dr M. Hayden (Plant Functional Genomics Centre, University of Adelaide), Dr J. Zhu, and Dr J. Yang (Zhejiang University) for valuable discussions. Funding was provided by the Value Added Wheat CRC and Grains Research and Development Corporation.
Bailey PC,
McKibbin RS,
Lenton JR,
Holdsworth MJ,
Flintham JE, Gale MD
(1999) Genetic map locations for orthologous Vp1 genes in wheat and rice. Theoretical and Applied Genetics 98, 281–284.
| Crossref | GoogleScholarGoogle Scholar |
Bink MCAM,
Te Pas MFW,
Harders FL, Janss LLG
(2000) A transmission/disequilibrium test approach to screen for quantitative trait loci in two selected lines of Large White pigs. Genetical Research 75, 115–121.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Buchanan AM, Nicholas EM
(1980) Sprouting, alpha-amylase and breadmaking quality. Cereal Research Communications 8, 23–28.
Cai HW, Morishima H
(2000) Genomic regions affecting seed shattering and seed dormancy in rice. Theoretical and Applied Genetics 100, 840–846.
| Crossref | GoogleScholarGoogle Scholar |
Carrari F,
Perez-Flores L,
Lijavetzky D,
Enciso S,
Sanchez R,
Benech-Arnold R, Iusem N
(2001) Cloning and expression of a sorghum gene with homology to maize vp1: its potential involvement in pre-harvest sprouting resistance. Plant Molecular Biology 45, 631–640.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Flintham J,
Adlam R,
Bassoi M,
Holdsworth M, Gale M
(2002) Mapping genes for resistance to sprouting damage in wheat. Euphytica 126, 39–45.
| Crossref | GoogleScholarGoogle Scholar |
Gale MD, Devos KM
(1998) Comparative genetics in the grasses. Proceedings of the National Academy of Sciences of the United States of America 95, 1971–1974.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Groos C,
Gay G,
Perretant MR,
Gervais L,
Bernard M,
Dedryver F, Charmet G
(2002) Study of the relationship between pre-harvest sprouting and grain color by quantitative trait loci analysis in a white × red grain bread-wheat cross. Theoretical and Applied Genetics 104, 39–47.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Hayden MJ,
Stephenson P,
Logojan AM,
Khatkar D,
Rogers C,
Elsden J,
Koebner RMD,
Snape JW, Sharp PJ
(2006) Development and genetic mapping of sequence tagged microsatellites (STMs) in bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics In press ,
| Crossref | GoogleScholarGoogle Scholar |
Hayden MJ,
Stephenson P,
Logojan AM,
Khatkar D,
Rogers C,
Koebner RMD,
Snape JW, Sharp PJ
(2004) A new approach to extending the wheat marker pool by anchored PCR amplification of compound SSRs. Theoretical and Applied Genetics 108, 733–742.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Jones HD,
Peters NCB, Holdsworth MJ
(1997) Genotype and environment interact to control dormancy and differential expression of the VIVIPAROUS 1 homologue in embryos of Avena fatua. The Plant Journal 12, 911–920.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Kato K,
Nakamura W,
Tabiki T,
Miura H, Sawada S
(2001) Detection of loci controlling seed dormancy on group 4 chromosomes of wheat and comparative mapping with rice and barley genomes. Theoretical and Applied Genetics 102, 980–985.
| Crossref | GoogleScholarGoogle Scholar |
Lehmensiek A,
Campbell AW,
Williamson PM,
Michalowitz M,
Sutherland MW, Daggard GE
(2004) QTLs for black-point resistance in wheat and the identification of potential markers for use in breeding programs. Plant Breeding 123, 410–416.
| Crossref | GoogleScholarGoogle Scholar |
Lijavetzky D,
Martinez MC,
Carrari F, Hopp HE
(2000) QTL analysis and mapping of pre-harvest sprouting resistance in sorghum. Euphytica 112, 125–135.
| Crossref | GoogleScholarGoogle Scholar |
Lin SY,
Sasaki T, Yano M
(1998) Mapping quantitative trait loci controlling seed dormancy and heading date in rice, Oryza sativa L., using backcross inbred lines. Theoretical and Applied Genetics 96, 997–1003.
| Crossref | GoogleScholarGoogle Scholar |
Lohwasser U,
Röder MS, Börner A
(2005) QTL Mapping of the domestication traits pre-harvest sprouting and dormancy in wheat (Triticum aestivum L.). Euphytica 143, 247–249.
| Crossref | GoogleScholarGoogle Scholar |
Manly KF,
Cudmore RH, Meer JM
(2001) Map Manager QTX, cross-platform software for genetic mapping. Mammalian Genome 12, 930–932.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Mares D,
Mrva K,
Cheong J,
Williams K,
Watson B,
Storlie E,
Sutherland M, Zou Y
(2005) A QTL located on chromosome 4A associated with dormancy in white- and red-grained wheats of diverse origin. Theoretical and Applied Genetics 111, 1357–1364.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Mares D,
Mrva K,
Tan M-K, Sharp P
(2002) Dormancy in white-grained wheat: progress towards identification of genes and molecular markers. Euphytica 126, 47–53.
| Crossref | GoogleScholarGoogle Scholar |
McCarty DR,
Hattori T,
Carson CB,
Vasil V,
Lazar M, Vasil IK
(1991) The Viviparous-1 developmental gene of maize encodes a novel transcriptional activator. Cell 66, 895–905.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
McKibbin RS,
Wilkinson MD,
Bailey PC,
Flintham JE,
Andrew LM,
Lazzeri PA,
Gale MD,
Lenton JR, Holdsworth MJ
(2002) Transcripts of Vp1 homeologues are misspliced in modern wheat and ancestral species. Proceedings of the National Academy of Sciences of the United States of America 99, 10203–10208.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Osa M,
Kato K,
Mori M,
Shindo C,
Torada A, Miura H
(2003) Mapping QTLs for seed dormancy and the Vp1 homologue on chromosome 3A in wheat. Theoretical and Applied Genetics 106, 1491–1496.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Röder MS,
Korzun V,
Wendehake K,
Plaschke J,
Tixier M,
Leroy P, Ganal MW
(1998) A microsatellite map of wheat. Genetics 149, 2007–2023.
| PubMed |
Spielman RS,
McGinnis RE, Ewens WJ
(1993) Transmission test for linkage disequilibrium: the insulin gene region and insulin-dependent diabetes mellitus (IDDM). American Journal of Human Genetics 52, 506–516.
| PubMed |
Tan MK, Medd RW
(2002) Characterisation of the acetolactate synthase (ALS) gene of Raphanus raphanistrum L. and the molecular assay of mutations associated with herbicide resistance. Plant Science 163, 195–205.
| Crossref | GoogleScholarGoogle Scholar |
Torada A,
Ikeguchi S, Koike M
(2005) Mapping and validation of PCR-based markers associated with a major QTL for seed dormancy in wheat. Euphytica 143, 251–255.
| Crossref | GoogleScholarGoogle Scholar |
Van der Schaar W,
Alonso-Blanco C,
Leon-Kloosterziel KM,
Jansen RC,
Van Ooijen JW, Koornneef M
(1997) QTL analysis of seed dormancy in Arabidopsis using recombinant inbred lines and MQM mapping. Heredity 79, 190–200.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Wenzl P,
Carling J,
Kudrna D,
Jaccoud D,
Huttner E,
Kleinhofs A, Kilian A
(2004) Diversity Arrays Technology (DArT) for whole-genome profiling of barley. Proceedings of the National Academy of Sciences of the United States of America 101, 9915–9920.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Yang J, Zhu J
(2005) Methods for predicting superior genotypes under multiple environments based on QTL effects. Theoretical and Applied Genetics 110, 1268–1274.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
