Seed priming with boron and Bacillus sp. MN54 inoculation improves productivity and grain boron concentration of chickpea
Noman Mehboob A , Waqas Ahmed Minhas A , Muhammad Naeem A , Tauqeer Ahmad Yasir B , Muhammad Naveed C , Shahid Farooq
D and Mubshar Hussain A E *
+ Author Affiliations
- Author Affiliations
A Department of Agronomy, Bahauddin Zakariya University, Multan, Pakistan.
B College of Agriculture, Bahauddin Zakariya University, Bahadur Sub-Campus, Layyah, Pakistan.
C Institute of Soil and Environmental Sciences, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan.
D Department of Plant Protection, Faculty of Agriculture, Harran University, Şanlıurfa, Turkey.
E School of Veterinary and Life Sciences, Murdoch University, 90 South Street, Murdoch, WA 6150, Australia.
Crop & Pasture Science 73(5) 494-502 https://doi.org/10.1071/CP21377
Submitted: 30 November 2020 Accepted: 5 August 2021 Published: 16 February 2022
© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
Context: The production of chickpea (Cicer arietinum L.) is negatively affected by boron (B) deficiency. In Pakistan, the crop grown under B deficiency produces grains with low B concentration. Application of B-tolerant bacteria (BTB) is a promising option to improve B supply to plants grown under B deficiency.
Aims: This study was focused on determining the appropriate concentration of B for seed priming, and its effects with BTB inoculation on growth, productivity and grain B concentration of chickpea.
Methods: Chickpea seeds were primed in aerated solutions of B concentrations in the range 0.01–0.5% (w/v), with hydroprimed and dry seeds as controls. Concentrations >0.1% proved toxic and seeds failed to germinate. Hence, B was further diluted to concentrations in the range 0.0001–0.1%. Pots containing chickpea seeds were divided into two sets having all B treatments. One set was inoculated with BTB (5 mL per pot of pure Bacillus sp. MN54 culture at 10 9 cfu mL−1); the other set was not inoculated.
Key results: Seed priming with B along with BTB inoculation improved stand establishment, growth, nodulation, yield and grain B concentration of chickpea. Seed priming treatments with B at 0.001% and 0.0001% along with BTB inoculation were most effective for improving stand establishment, seedling growth and grain yield, whereas 0.1% B was more effective for improving grain B concentration.
Conclusions: Seed priming with 0.001% B along with inoculation of Bacillus sp. MN54 improved seed germination, nodulation, yield and grain B concentration of chickpea under B-deficiency conditions.
Implications: Seed inoculation with BTB i.e., Bacillus sp. MN54 coupled with seed priming in 0.001% B solution is a viable option to improve yield and grain B concentration of chickpea grown on B-deficient soils.
Keywords: Cicer arietinum, grain biofortification, growth, nodulation, seedling establishment, seed priming, yield, 100-grain weight.
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