Identification of quantitative trait loci associated with salt tolerance during seedling growth in soybean (Glycine max L.)
Huatao Chen A , Shiyou Cui A B , Sanxiong Fu A , Junyi Gai A and Deyue Yu A CA National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
B Jiangsu Yanjiang Institute of Agricultural Sciences, Nantong 226541, China.
C Corresponding author. Email: dyyu@njau.edu.cn
Australian Journal of Agricultural Research 59(12) 1086-1091 https://doi.org/10.1071/AR08104
Submitted: 23 March 2008 Accepted: 15 September 2008 Published: 10 November 2008
Abstract
Salt stress is an important factor affecting the growth and development of soybean. The inheritance and expression of traits associated with salt tolerance during the seedling stage are complex. The present study was conducted to identify quantitative trait loci (QTLs) associated with salt tolerance during seedling growth in soybean. Field and greenhouse experiments were conducted to evaluate 184 recombinant inbred lines (RILs) derived from a cross between Kefeng No. 1 and Nannong1138-2 for salt tolerance and QTLs that are associated with salt tolerance. The molecular map of this RIL population, covering 2625.9 cM of the genome, converged into 24 linkage groups and consisted of 221 SSR markers and 1 disease-resistant gene (Rsc-7). QTL mapping was conducted using WinQTLCart. Eight putative QTLs significantly associated with salt tolerance were identified. One QTL was identified both in field and greenhouse experiments. In the field, salt tolerance was assessed (tolerance rating, TR) visually on a 0 (death) to 5 (unaffected by salt stress) scale. Three QTLs were detected on two linkage groups explaining 7.1–19.7% of the total phenotypic variance for salt tolerance. In the greenhouse, plant survival days (PSD) and percentage of plant survival (PPS) under salt stress were measured. Six QTLs were detected on six linkage groups, and explained 7.8–19.2% of total phenotypic variation for salt tolerance. A major QTL was identified between markers Sat_164 and Sat_358 on linkage group G in both the field and greenhouse. This QTL qppsN.1 was identified in the same location as a salt tolerance QTL previously reported in soybean. The detection of new QTLs will provide important information for marker-assisted selection (MAS) and further genetic studies on salt tolerance in soybean.
Additional keywords: simple sequence repeat (SSR), recombinant inbred line (RIL).
Acknowledgments
We thank Dr Jill Cairns from the International Rice Research Institute for careful reading and correction of this manuscript. This work was supported in part by grants from the International Atomic Energy Agency (CRP-12988), National 973 project (2004CB117206), 863 project (No. 2006AA10Z1C1), and the 111 Project from the Ministry of Education of China (B08025).
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