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RESEARCH ARTICLE
Contents Vol 57(11)

The effect of time off feed prior to slaughter on muscle glycogen metabolism and rate of pH decline in three different muscles of stimulated and non-stimulated sheep carcasses

B. L. Daly A C , G. E. Gardner A , D. M. Ferguson B and J. M. Thompson A
+ Author Affiliations
- Author Affiliations

Cooperative Research Centre for Cattle and Beef Quality

A School of Rural Science and Agriculture, University of New England, Armidale, NSW 2351, Australia.

B CSIRO Chiswick Livestock Research Facility, Armidale, NSW 2350, Australia.

C Corresponding author. Email: bdaly.hannwag@bigpond.com.au

Australian Journal of Agricultural Research 57(11) 1229-1235 https://doi.org/10.1071/AR05424
Submitted: 2 December 2005  Accepted: 17 July 2006   Published: 27 October 2006

Abstract

The aim of this study was to determine the effect of time off feed (TOF) prior to slaughter on muscle glycogen metabolism and rate of pH decline in sheep muscle. All animals were maintained on a roughage diet for 6 weeks and were then subjected to either 0, 2, or 4 days TOF with access to water, prior to slaughter. Glycogen concentrations were determined post-slaughter for 3 different muscles, M. longissimus thoracis et lumborum (LTL), M. semimembranosus (SM), and M. semitendinosus (ST), as well as measuring pH declines for all animals in each of the 3 muscles under both electrically stimulated and control conditions. Ultimate pH values (pHu) were determined 48 h post-slaughter. Both the 2-day and 4-day TOF groups lost liveweight during their curfew period, whereas the control (0-day) group gained weight. TOF had no effect on post-slaughter carcass characteristics, muscle glycogen concentrations, pHu, or rate of pH decline. Increased muscle glycogen concentrations resulted in faster rates of pH decline. This response was curvilinear, plateauing at a glycogen concentration of about 56 mmol/kg muscle. Muscle glycogen concentration also affected the response of pH decline to electrical stimulation, interacting with muscle and pre-stimulation pH. Low muscle glycogen levels limited delta pH only in the SM and ST and only in muscles of lower pre-stimulation pH.


Acknowledgments

The authors acknowledge the efforts of staff at the CSIRO ‘Chiswick’ Livestock Facility, Armidale; the Meat Science Department at the University of New England, Armidale; and Food Science Australia, Brisbane; for their assistance in the running of this experiment.


References


Aalhus JL, Price MA (1991) Endurance-exercised growing in sheep: I. Post-mortem and histological changes in skeletal muscles. Meat Science 29, 43–56.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Bendall JR (1978) Variability in rates of pH fall and of lactate production in the muscles on cooling beef carcasses. Meat Science 2, 91–104.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Bendall JR, Wismer-Pedersen J (1962) Some properties of the fibrillar proteins of normal and watery pork muscle. Journal of Food Science 27, 144–159.
| Crossref |
open url image1

Bruce HL, Scott JR, Thompson JM (2001) Application of an exponential model to early post-mortem bovine muscle pH decline. Meat Science 58, 39–44.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Chrystall BB, Devine CE (1978) Electrical stimulation, muscle tension and glycolysis in bovine Sternomandibularis. Meat Science 2, 49–58.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Crouse JD, Smith SB, Prior RL (1984) Bovine muscle glycogen as affected by fasting and refeeding. Journal of Animal Science 59, 384–387.
| PubMed |
open url image1

Daly BL , Richards I , Gibson PG , Gardner GE , Thompson JM (2002) Rate of pH decline in bovine muscle post-mortem—a benchmarking study. In ‘Proceedings of the 48th ICoMST’. Rome, Italy. Vol. 2, pp. 560–561.

De Boer SF, Koopmans SJ, Slangen JL, van der Gugten J (1989) Effects of fasting on plasma catecholamine, corticosterone and glucose concentrations under basal and stress conditions in individual rats. Physiology & Behavior 45, 989–994.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Devine CE, Ellery S, Averill S (1984) Responses of different types of ox muscle to electrical stimulation. Meat Science 10, 35–51.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Dransfield E, Etherington DJ, Taylor MAJ (1992) Modelling post-mortem tenderization. II: Enzyme changes during storage of electrically stimulated and non-stimulated beef. Meat Science 31, 75–84.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Ferguson DM, Bruce HL, Thompson JM, Egan AF, Perry D, Shorthose WR (2001) Factors affecting beef palatability—farmgate to chilled carcass. Australian Journal of Experimental Agriculture 41, 879–891.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Fernandez X, Meunier-Salaun M-C, Ecolan P, Mormede P (1995) Interactive effect of food deprivation and agonistic behavior on blood parameters and muscle glycogen in pigs. Physiology & Behavior 58, 337–345.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Gardner GE, McIntyre BL, Tudor GD, Pethick DW (2001) The impact of nutrition on bovine muscle glycogen metabolism following exercise. Australian Journal of Agricultural Research 52, 461–470.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Hintz CS, Chi MMY, Fell RD, Ivy JL, Kaiser KK, Lowry CV, Lowry OH (1982) Metabolite changes in individual rat muscle fibers during stimulation. American Journal of Physiology 242, C218–C228.
| PubMed |
open url image1

Howard A (1963) The relation between physiological stress and meat quality. In ‘Carcase composition and appraisal of meat animals’. (Ed. DE Tribe) (CSIRO Australia: Melbourne)

Hunt MC, Hedrick HB (1977) Profile of fiber types and related properties of five bovine muscles. Journal of Food Science 42, 513–517.
| Crossref |
open url image1

Immonen K, Puolanne E (2000) Variation of residual glycogen-glucose concentration at ultimate pH values below 5.75. Meat Science 55, 279–283.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Immonen K, Ruusunen M, Hissa K, Puolanne E (2000) Bovine muscle glycogen concentration in relation to finishing diet, slaughter and ultimate pH. Meat Science 55, 25–31.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Jacob R, Pethick D, Chapman H (2005) Muscle glycogen concentrations in commercial consignments of Australian lamb measured on farm and post slaughter after 3 different lairage periods. Australian Journal of Experimental Agriculture 45, 543–552.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Klont RE, Brocks L, Eikelenboom G (1998) Muscle fibre type and meat quality. Meat Science 49, S219–S229.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Kunst A , Draeger B , Ziegenhorn J (1983) ‘Methods of enzymatic analysis.’ (Ed. HU Bergmeyer) pp. 163–172. (Verlag Chemie: Deerfield, FL)

Marbach EP, Weil MH (1967) Rapid enzymatic measurement of blood lactate and pyruvate. Use and significance of metaphosphoric acid as a common precipitant. Journal of Clinical Chemistry 13, 314–325. open url image1

Pethick DW, Cummins L, Gardner GE, Knee BW, McDowell M, McIntyre BL, Tudor GD, Walker PJ, Warner RD (2000) The regulation of glycogen level in the muscle of ruminants by nutrition. Proceedings of the New Zealand Society of Animal Production 60, 94–97. open url image1

Pethick DW, Lindsay DB, Barker PJ, Northrop AJ (1983) Metabolism of circulating non-esterified fatty acids by the whole animal, hindlimb, and uterus of pregnant ewes. British Journal of Nutrition 49, 129–143.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Pethick DW, Rowe JB (1996) The effect of nutrition and exercise on carcass parameters and the level of glycogen in skeletal muscle. Australian Journal of Agricultural Research 47, 525–537.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Randle PJ, Garland PB, Hales CN, Newsholme EA (1963) The glucose fatty-acid cycle: its role in insulin sensitivity and the metabolic disturbances of diabetes mellitus. Lancet 281, 785–789.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Richter E, Galbo H (1986) High glycogen levels enhance glycogen breakdown in isolated contracting skeletal muscle. Journal of Applied Physiology 61, 827–831.
| PubMed |
open url image1

SAS Institute (2001) ‘Statistical analysis system Version 8.’ (SAS Institute Inc.: Cary, NC)

Tarrant PV (1989) Animal behaviour and environment in the dark-cutting condition in beef – a review. Irish Journal of Food Science and Technology 13, 1–21. open url image1

Tudor GD, Coupar FJ, Pethick DW (1996) Effect of silage on glycogen concentration in the muscle of yearling steers. Proceedings of the Australian Society of Animal Production 21, 451. open url image1

Warriss PD (1990) The handling of cattle pre-slaughter and its effects on carcass and meat quality. Applied Animal Behaviour Science 28, 171–186.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Weick BG, Ritter S, McCarty R (1983) Plasma catecholamines in fasted and sucrose supplemented rats. Physiology & Behavior 30, 247–252.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Wronska D, Niezgoda J, Sechman A, Bobek S (1990) Food deprivation suppresses stress-induced rise in catabolic hormones with a concomitant tendency to potentiate the increment of blood glucose. Physiology & Behavior 48, 531–537.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1