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RESEARCH ARTICLE
Contents Vol 57(9)

Confirmation of QTL mapping and marker validation for partial seedling resistance to crown rot in wheat line ‘2-49’

B. C. Y. Collard A C , R. Jolley A , W. D. Bovill A , R. A. Grams A , G. B. Wildermuth B and M. W. Sutherland A D
+ Author Affiliations
- Author Affiliations

A Centre for Systems Biology (formerly, Centre for Rural and Environmental Biotechnology), Faculty of Sciences, University of Southern Queensland, Toowoomba, Qld 4350, Australia.

B Leslie Research Centre, Queensland Department of Primary Industries and Fisheries, Toowoomba, Qld 4350, Australia.

C Present address: Hermitage Research Station, Queensland Department of Primary Industries and Fisheries, Warwick, Qld 4370, Australia.

D Corresponding author. Email: marksuth@usq.edu.au

Australian Journal of Agricultural Research 57(9) 967-973 https://doi.org/10.1071/AR05419
Submitted: 28 November 2005  Accepted: 27 April 2006   Published: 30 August 2006

Abstract

We have tested the efficacy of putative microsatellite single sequence repeat (SSR) markers, previously identified in a 2-49 (Gluyas Early/Gala) × Janz doubled haploid wheat (Triticum aestivum) population, as being linked to partial seedling resistance to crown rot disease caused by Fusarium pseudograminearum. The quantitative trait loci (QTLs) delineated by these markers have been tested for linkage to resistance in an independent Gluyas Early × Janz doubled haploid population. The presence of a major QTL on chromosome 1DL (QCr.usq-1D1) and a minor QTL on chromosome 2BS (QCr.usq-2B1) was confirmed. However, a putative minor QTL on chromosome 2A was not confirmed. The QTL on 1D was inherited from Gluyas Early, a direct parent of 2-49, whereas the 2B QTL was inherited from Janz. Three other putative QTLs identified in 2-49 × Janz (on 1AL, 4BL, and 7BS) were inherited by 2-49 from Gala and were not able to be confirmed in this study. The screening of SSR markers on a small sample of elite wheat genotypes indicated that not all of the most tightly linked SSR markers flanking the major QTLs on 1D and 1A were polymorphic in all backgrounds, indicating the need for additional flanking markers when backcrossing into some elite pedigrees. Comparison of SSR haplotypes with those of other genotypes exhibiting partial crown rot resistance suggests that additional, novel sources of crown rot resistance are available.

Additional keywords: targetted mapping, QTL resolution, 1-LOD support interval, allelic homoplasy.


Acknowledgments

We thank Matt Davis, Sally Coverdale, and Boyd McNamara (Leslie Research Centre, Toowoomba) for excellent technical assistance with the glasshouse experiments. We also thank the Grains Research and Development Corporation (GRDC) for providing funding for this research within the Australian Winter Cereals Molecular Marker Program.


References


Appels R (2003) A consensus molecular genetic map for wheat – a cooperative international effort. In ‘Proceedings of the 10th International Wheat Genetics Symposium’. Paestum, Italy. (Ed. NE Progna) (Instituto Sperimentale per la Cerealicoltura: Rome, Paestum, Italy)

Beavis W (1998) QTL analyses: power, precision and accuracy. In ‘Molecular dissection of complex traits’. (Ed. AH Paterson) (CRC Press: Boca Raton, FL)

Beddis AL, Burgess LW (1992) The influence of plant water stress on infection and colonization of wheat seedlings by Fusarium graminearum Group 1. Phytopathology 82, 78–83. open url image1

Bovill WD, Ma W, Ritter K, Collard BCY, Davis M, Wildermuth GB, Sutherland MW (2006) Identification of novel QTLs for resistance to crown rot in the doubled haploid wheat population ‘W21MMT70’ × ‘Mendos’. Plant Breeding In press , open url image1

Burgess LW Backhouse D Summerell BA Swan LJ (2001) Crown rot of wheat. In ‘Fusarium’. (Eds BA Summerell, JF Leslie, D Backhouse, WL Bryden, LW Burgess) pp. 271–294. (APS Press: St Paul, MN)

Collard BCY, Grams RA, Bovill WD, Percy C, Jolley R, Lehmensiek A, Wildermuth G, Sutherland MW (2005) Development of molecular markers for crown rot resistance in wheat: mapping of QTLs for seedling resistance in a ‘2-49’ × ‘Janz’ population. Plant Breeding 124, 532–537.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Grimaldi MC, Crouau-Roy B (1997) Microsatellite allelic homoplasy due to variable flanking sequences. Journal of Molecular Evolution 44, 336–340.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Gupta PK, Balyan HS, Edwards K, Isaac P, Korzun V, Rader M, Gautier M-F, Joudrier P, Schlatter A, Dubcovsky J, De la Pena R, Khairallah M, Penner G, Hayden M, Sharp P, Keller B, Wang R, Hardouin J, Jack P, Leroy P (2002) Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theoretical and Applied Genetics 105, 413–422.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Harker N, Rampling LR, Shariflou MR, Hayden MJ, Holton TA, Morell MK, Sharp PJ, Henry RJ, Edwards KJ (2001) Microsatellites as markers for Australian wheat improvement. Australian Journal of Agricultural Research 52, 1121–1130.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Hayden MJ, Kuchel H, Chalmers KJ (2004) Sequence tagged microsatellites for the Xgwm533 locus provide new diagnostic markers to select for the presence of stem rust resistance gene Sr2 in bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics 109, 1641–1647.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Hyne V, Kearsey MJ, Pike DJ, Snape JW (1995) QTL analysis: unreliability and bias in estimation procedures. Molecular Breeding 1, 273–282.
| Crossref |
open url image1

Jansen RC (1994) Controlling the Type 1 and Type 2 errors in mapping quantitative trait loci. Genetics 138, 871–881.
| PubMed |
open url image1

Jansen RC (2001) Quantitative trait loci in inbred lines. In ‘Handbook of statistical genetics’. (Eds DJ Balding, M Bishop, C Cannings) pp. 567–597. (Wiley: New York)

Kearsey MJ, Farquhar GL (1998) QTL analysis in plants; where are we now? Heredity 80, 137–142.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Lander ES, Kruglyak L (1995) Genetic dissection of complex traits: guidelines for interpreting and reporting linkage results. Nature Genetics 11, 241–247.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Langridge P, Lagudah E, Holton T, Appels R, Sharp P, Chalmers K (2001) Trends in genetic and genome analyses in wheat: a review. Australian Journal of Agricultural Research 52, 1043–1077.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Liu C , Mitter V , Mager T , Ban T , Chakraborty S (2004) Relationships between resistance to Fusarium head blight and crown rot in hexaploid wheat. In ‘4th International Crop Science Congress’. 26 Sept.–1 Oct. 2004, Brisbane, Australia. (The Regional Institute Limited: Brisbane, Qld)

Liu S, Anderson JA (2003) Marker assisted evaluation of Fusarium head blight resistant wheat germplasm. Crop Science 43, 760–766. open url image1

Manly KF, Cudmore Robert H, Meer JM (2001) Map Manager QTX, cross-platform software for genetic mapping. Mammalian Genome 12, 930–932.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

McCartney CA, Somers DJ, Fedak G, Cao W (2004) Haplotype at diversity Fusarium head blight resistance QTLs in wheat. Theoretical and Applied Genetics 109, 261–271.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Melchinger AE, Utz HF, Schon CC (1998) Quantitative trait locus (QTL) mapping using different testers and independent population samples in maize reveals low power of QTL detection and large bias in estimates of QTL effects. Genetics 149, 383–403.
| PubMed |
open url image1

Pestsova E, Ganal MW, Roder MS (2000) Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome 43, 689–697.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Roder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149, 2007–2023.
| PubMed |
open url image1

Sharp PJ, Johnston S, Brown G, McIntosh RA, Pallotta M, Carter M, Bariana HS, Khatkar S, Lagudah ES, Singh RP, Khairallah M, Potter R, Jones MGK (2001) Validation of molecular markers for wheat breeding. Australian Journal of Agricultural Research 52, 1357–1366.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics 109, 1105–1114.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Spielmeyer W, Sharp PJ, Lagudah ES (2003) Identification and validation of markers linked to broad-spectrum stem rust resistance gene Sr2 in wheat (Triticum aestivum L.). Crop Science 43, 333–336. open url image1

Voorrips RE (2002) MapChart: Software for the graphical presentation of linkage maps and QTLs. Journal of Heredity 93, 77–78.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Wallwork H, Butt M, Cheong JPE, Williams KJ (2004) Resistance to crown rot in wheat identified through an improved method for screening adult plants. Australasian Plant Pathology 33, 1–7.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Wilcox MC, Khairallah MM, Bergvinson D, Crossa J, Deutsch JA, Edmeades GO, Gonzalez-de-Leon D, Jiang C, Jewell DC, Mihm JA, Williams WP, Hoisington D (2002) Selection for resistance to southwestern corn borer using marker-assisted and conventional backcrossing. Crop Science 42, 1516–1528. open url image1

Wildermuth GB, McNamara RB (1994) Testing wheat seedlings for resistance to crown rot caused by Fusarium graminearum Group 1. Plant Disease 78, 949–953. open url image1

Wildermuth GB, McNamara RB, Quick JS (2001) Crown depth and susceptibility to crown rot in wheat. Euphytica 122, 397–405.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Zhou W-C, Kolb FL, Bai G-H, Domier LL, Boze LK, Smith NJ (2003) Validation of a major QTL for scab resistance with SSR markers and use of marker-assisted selection in wheat. Plant Breeding 122, 40–46.
| Crossref | GoogleScholarGoogle Scholar | open url image1