Correlation of important seedling traits in cauliflower varieties and potential association with RAPD markers
Ida A. Astarini A B D , Julie A. Plummer A , Rachel A. Lancaster C and Guijun Yan AA Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
B Biology Department, Faculty of Mathematics and Natural Sciences, Udayana University, Bali, Indonesia.
C Department of Agriculture, Western Australia, Bunbury District Office, PO Box 1231, Bunbury, WA 6231, Australia.
D Corresponding author. Email: arin@student.uwa.edu.au
Australian Journal of Agricultural Research 58(12) 1183-1190 https://doi.org/10.1071/AR05417
Submitted: 26 November 2005 Accepted: 19 July 2007 Published: 17 December 2007
Abstract
Cauliflower production is hindered by variation in curd quality and maturity. Morphological variation from seed to harvest is due to genetic variation interacting with environmental conditions and here the genetic factors were investigated. The aim of this study was to search for DNA markers linked to seedling traits, facilitating early selection of seedlings in cauliflower production. Cauliflower seed lines were germinated in Petri dishes (20°C, 7 days) and seedlings were transferred to pots and grown under glasshouse conditions. Seed weight and various seedling characters were measured until harvest at 6 weeks. DNA was extracted using the CTAB method and RAPD markers were identified using 17 primers. Multivariate analysis based on principal coordinates analysis was used to correlate morphological traits with molecular markers across cultivars. Markers associated with seed weight, germination rate, shoot length, root length, fresh weight, and dry weight were identified.
Additional keyword: Brassica oleracea var. botrytis.
Acknowledgments
We thank AusAID for providing a scholarship to Ida Astarini. Sincere thanks to Bejo, Syngenta, Enza Zaden (formerly Yates Seeds), South Pacific Seeds, and Henderson Seeds for providing seeds for this project. Financial assistance from the Department of Agriculture Western Australia is gratefully acknowledged.
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