Metabolism of Semen After Freezing I. Effects of Washing, Skim-Milk Solids, and Potassium and Magnesium Ions On The Metabolism of Thawed Ram and Bull Spermatozoa

Abstract

Ram spermatozoa after storage at -79°C were damaged when washed twice 10 10 volumes of diluent, the procedure used for recently ejaculated cells. After freezing, the oxygen uptake of spermatozoa washed once in 10 volumes of diluent was the same as that of unwashed cells. Using this degree of washing it was not possible to obtain thawed cells free of fructose and lactate.