Further Studies of The Deep Freezing of Rabbit Spermatozoa In Reconstituted Skim Milk Powder

Abstract

Using motility as the criterion of survival of spermatozoa, a method for the deep-frozen storage of rabbit semen was developed. The factors found to give optimum revival were the presence of 14% (v/v) dimethylsulphoxide during freezing, the inclusion of fructose in the diluent, and cooling to 5°C in an isotonic diluent while freezing in a hypertonic diluent.