Effect of Propionibacterium freudenreichii in diets containing rapeseed or flaxseed oil on in vitro ruminal fermentation, methane production and fatty acid biohydrogenation
S. Ding A B , S. J. Meale A B G , A. Y. Alazzeh C B , M. L. He B , G. O. Ribeiro
+ Author Affiliations
- Author Affiliations
A Faculty of Veterinary Science, University of Sydney, Sydney, NSW 2006, Australia.
B Lethbridge Research Center, Agriculture and Agri-Food Canada, 5403-1st Avenue South, Lethbridge, Alberta, Canada, T1J 4B1.
C Department of Clinical Nutrition, Faculty of Applied Medical Sciences, University of Ha’il Ha’il, Saudi Arabia.
D CAPES Foundation, Ministry of Education of Brazil, Brasilia, Brazil.
E Department of Animal Science, Northeast Agricultural University, No.59 Mucai Street, XiangFang District, Harbin, Heilongjiang, China.
F Lacombe Research Center, Agriculture and Agri-Food Canada, 6000C and E Trail, Lacombe, Alberta, Canada, T4L 1W1.
G Present address: INRA, UR1213 Herbivores, 63122 Site de Theix, Saint-Genés-Champanelle, France.
H Corresponding author. Email: Tim.McAllister@agr.gc.ca
Animal Production Science 57(10) 2051-2059 https://doi.org/10.1071/AN15878
Submitted: 25 March 2015 Accepted: 2 August 2016 Published: 5 September 2016
Abstract
The objectives of the present study were to determine the effect of inoculating Propionibacterium freudenreichii subsp. shermanii ATCC 8262 (1 × 109 colony-forming units per vial) in a barley silage-based diet supplemented with flaxseed oil or rapeseed oil (60 g/kg DM), on in vitro proportions and yield of volatile fatty acids, methane production and fatty acid (FA) biohydrogenation. Total volatile fatty acid production (mM) and proportions of individual FAs were not affected (P ≥ 0.10) by P. freudenreichii. Similarly, propionibacteria had little impact on FA biohydrogenation, resulting only in an increased accumulation (P < 0.01) of C18:1 cis-15 (g/kg total FA) at 6 h of incubation, compared with the control (CON). Compared with the CON, an increased (P < 0.01) accumulation of vaccenic acid was observed at 48 h in all oil-containing treatments, regardless of the oil type. Similarly, the apparent biohydrogenation of flaxseed oil resulted in an increased (P ≤ 0.04) accumulation of conjugated linoleic acid cis-9, trans-11, compared with all other treatments. Additionally, flaxseed oil produced a greater (P ≤ 0.01) accumulation of beneficial biohydrogenation intermediates (C18:2 trans-11, cis-15; C18:1 cis-15 and vaccenic acid), reflecting its ability to produce a more desirable FA profile than that of rapeseed oil or CON. The inability of P. freudenreichii subsp. shermanii ATCC 8262 to alter ruminal fermentation in a manner that lowered methane production, along with only minor effects on FA profiles through biohydrogenation, suggests that the biological activity of this strain was not realised under in vitro batch-culture conditions.
Additional keywords: barley silage, batch culture, cattle, direct-fed microbial, probiotic, propionibacteria, rumen.
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