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RESEARCH ARTICLE

Factors influencing the occurrence of high ultimate pH in three muscles of lamb carcasses in Australia

N. G. McPhail A , J. L. Stark A D , A. J. Ball B and R. D. Warner C
+ Author Affiliations
- Author Affiliations

A CSIRO Animal, Food and Health Sciences, 39 Kessels Road, Coopers Plains, Qld 4108, Australia.

B Meat and Livestock Australia, CJ Hawkins Homestead Building, University of New England, Armidale, NSW 2351, Australia.

C Melbourne School of Land and Environment, Melbourne University, Parkville, Vic. 3052, Australia.

D Corresponding author. Email: janet.stark@csiro.au

Animal Production Science 54(10) 1853-1859 https://doi.org/10.1071/AN14315
Submitted: 13 March 2014  Accepted: 19 June 2014   Published: 19 August 2014

Abstract

Chilled lamb meat exported from Australia has, on occasions, been rejected by importing countries due to greening, after only 6 weeks of storage time. Greening is known to be more prevalent in high ultimate pH (pHu) beef meat (>5.9). There are few data available for lamb carcasses in Australia on the occurrence of high pHu meat, which may have an impact on the understanding and control of quality and greening during storage. The aim of this project was to determine the prevalence of, and influencing factors for, high pHu meat in a range of muscle types in lamb carcasses in Australia. Muscle pHu data were collected from a total of 1614 carcasses from 78 lots at four lamb processing plants in Victoria and New South Wales in autumn and spring of 2013. The pHu of the knuckle (rectus femoris), rack (longissimus) and blade (infraspinatus) was measured and data on carcass and lot characteristics were recorded. Data were subjected to restricted maximum likelihood and generalised linear mixed model analysis. The mean pHu of the knuckle, rack and shoulder were 6.06, 5.79 and 6.12 respectively, and the main factors influencing muscle pHu and occurrence of dark-cutting were breed, season, electrical stimulation and carcass weight. Merino lambs had a higher pHu in the blade and knuckle than did other breeds (P < 0.05, P < 0.01 respectively). Lambs processed in autumn had a higher predicted pHu in the blade and knuckle and a higher percentage dark-cutting (DC; pHu >6.0) for those muscles, than did those processed in spring (P < 0.05). Carcasses that had been electrically stimulated had a higher %DC and a higher pHu in all three muscles (P < 0.05). Carcass weight had a significant effect on the pHu of all three muscles (P < 0.001), with heavier carcasses having a lower pHu and lower %DC. The pHu of the rack was not a reliable predictor for the pHu in other muscles of the lamb carcass. In conclusion, the high occurrence of DC in the muscles, particularly the blade and knuckle, suggests that these muscles may be at risk for producing greening in the vacuum bag during storage.

Additional keywords: breed, colour, dark-cutting, greening, season.


References

Brownlie LE (1989) Dark cutting meat in the export meat trade. In ‘Dark-cutting in cattle and sheep-proceedings of an Australian workshop. Report 89/02.’ (Eds SU Fabiansson, WR Shorthose, RD Warner) pp. 77. (Australian Meat and Livestock Research and Development Corporation: Sydney)

Channon HA, Warner RD (2011) Delivering consistent quality Australian pork to consumers – a systems approach. (Ed. RJ Barneveld) pp. 262–293. (Australasian Pig Science Association Inc.: Werribee, Vic.)

Devine CE 1994 Incidence of high pH beef and lamb. I: implications for meat quality. Proceedings Meat Industry Research Conference, Auckland, New Zealand 28 125 132

Devine CE, Chrystall BB (1989) High ultimate pH in sheep. In ‘Dark cutting in cattle and sheep. Proceedings of an Australian workshop. Report 89/02’. (Eds SU Fabiansson, WR Shorthose, RD Warner) pp. 55–65. (Australian Meat and Livestock Research and Development Corporation: Sydney)

Ferguson DM, Warner RD (2008) Have we underestimated the impact of pre-slaughter stress on meat quality in ruminants? Meat Science 80, 12–19.
Have we underestimated the impact of pre-slaughter stress on meat quality in ruminants?Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3MbnsFGhtw%3D%3D&md5=323d757bd1f8b7d692a3836e56312925CAS | 22063165PubMed |

Ferguson DM, Warner RD, Walker PJ, Knee B (2007) Effect of cattle marketing method on beef quality and palatability. Australian Journal of Experimental Agriculture 47, 774–781.
Effect of cattle marketing method on beef quality and palatability.Crossref | GoogleScholarGoogle Scholar |

Gardner GE, Kennedy L, Milton JTB, Pethick DW (1999) Glycogen metabolism and ultimate pH of muscle in Merino, first-cross, and second-cross wether lambs as affected by stress before slaughter. Australian Journal of Agricultural Research 50, 175–181.
Glycogen metabolism and ultimate pH of muscle in Merino, first-cross, and second-cross wether lambs as affected by stress before slaughter.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXhslCrs7s%3D&md5=acb7351768acf5b8f8c94566bdb0161fCAS |

Greenwood PL, Harden S, Hopkins DL (2007) Myofibre characteristics of ovine longissimus and semitendinosus muscles are influenced by sire breed, gender, rearing type, age and carcass weight. Australian Journal of Experimental Agriculture 47, 1137–1146.
Myofibre characteristics of ovine longissimus and semitendinosus muscles are influenced by sire breed, gender, rearing type, age and carcass weight.Crossref | GoogleScholarGoogle Scholar |

Hopkins DL, Fogarty NM (1998) Diverse lamb genotypes – 2. Meat pH, colour and tenderness. Meat Science 49, 477–488.
Diverse lamb genotypes – 2. Meat pH, colour and tenderness.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3MbntlCntQ%3D%3D&md5=8f193fee084577ad09c79a521c9a5c33CAS | 22060628PubMed |

Hopkins DL, Stanley DF, Martin LC, Toohey ES, Gilmour AR (2007) Genotype and age effects on sheep meat production. 3. Meat quality. Australian Journal of Experimental Agriculture 47, 1151–1164.

Jacob RH, Pethick DW, Chapman HM (2005) Muscle glycogen concentrations in commercial consignments of Australian lamb measured on farm and post-slaughter after three different lairage periods. Australian Journal of Experimental Agriculture 45, 543–552.
Muscle glycogen concentrations in commercial consignments of Australian lamb measured on farm and post-slaughter after three different lairage periods.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXlsFOmtbc%3D&md5=86c15fc40d04e9464b58aaf90cca762aCAS |

Knee BW, Cummins LJ, Walker PJ, Warner RD (2004) Seasonal variation in muscle glycogen in beef steers. Australian Journal of Experimental Agriculture 44, 729–734.
Seasonal variation in muscle glycogen in beef steers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXnslShurg%3D&md5=6d31d9cd66e7f13a5b92e37e7e05b083CAS |

Knee BW, Cummins LJ, Walker PJ, Kearney GA, Warner RD (2007) Reducing dark-cutting in pasture-fed beef steers by high-energy supplementation. Australian Journal of Experimental Agriculture 47, 1277–1283.
Reducing dark-cutting in pasture-fed beef steers by high-energy supplementation.Crossref | GoogleScholarGoogle Scholar |

Martin KM, Gardner GE, Thompson JM, Hopkins DL (2004) Nutritional impact on muscle glycogen metabolism in lambs selected for muscling. Journal of Animal and Feed Sciences 13, 639–642.

Meat and Livestock Australia (2014) ‘Sheep.’ Available at http://www.mla.com.au/Cattle-sheep-and-goat-industries/Industry-overview/Sheep. [Accessed 29 May 2014]

Monin G (1991) Muscle metabolic type and the DFD condition. In ‘Current topics in veterinary medicine and animal science. Vol. 10. The problem of dark-cutting in beef’. (Eds DE Hood, PV Tarrant) pp. 63–82. (Martinus-Nijhoff: The Hague, The Netherlands)

Meat Standards Australia (MSA) (2013) Meat Standards Australia annual outcomes report 2012–2013. http://www.mla.com.au/Marketing-beef-and-lamb/Meat-Standards-Australia.

Nicol DJ, Shaw MK, Ledward DA (1970) Hydrogen sulphide production by bacteria and sulphmyoglobin formation in prepacked chilled beef. Applied Microbiology 19, 937–939.

Ponnampalam EN, Warner RD, Dunshea FR (2012) Basal and hormone stimulated metabolism in lambs can vary with genotype and diet quality. Domestic Animal Endocrinology 42, 94–102.
Basal and hormone stimulated metabolism in lambs can vary with genotype and diet quality.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XmtFCqtg%3D%3D&md5=09e62f04d34ad988009b325b07a9e45dCAS | 22119112PubMed |

Purslow PP, Mandell IB, Widowski TM, Brown J, deLange CFM, Robinson JAB, Squires EJ, Cha MC, VanderVoort G (2008) Modelling quality variations in commercial Ontario pork production. Meat Science 80, 123–131.
Modelling quality variations in commercial Ontario pork production.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3MbnsFGgtQ%3D%3D&md5=5731bc5ad12aa810e477bfc55a20e2ebCAS | 22063177PubMed |

Robinson DL, Ferguson DM, Oddy VH, Perry D, Thompson JM (2001) Genetic and environmental influences on beef tenderness. Australian Journal of Experimental Agriculture 41, 997–1003.
Genetic and environmental influences on beef tenderness.Crossref | GoogleScholarGoogle Scholar |

Safari E, Channon HA, Hopkins DL, Hall DG, Van De Ven R (2002) A national audit of retail lamb loin quality in Australia. Meat Science 61, 267–273.
A national audit of retail lamb loin quality in Australia.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3MbntlKjuw%3D%3D&md5=ccbfcd813199c965b2fd27ad372dc59dCAS | 22060849PubMed |

Suzuki A, Tamate H (1988) Distribution of myofiber types in the hip and thigh musculature of sheep. The Anatomical Record 221, 494–502.
Distribution of myofiber types in the hip and thigh musculature of sheep.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaL1c3mslGjtw%3D%3D&md5=8652f7183c47fb841df8e1d719c133ccCAS | 2968770PubMed |

Tarrant PV (1989) The effect of handling, transport, slaughter and chilling on meat quality and yield of pigs. Manipulating Pig Production 3, 1–25.

Warner RD, Truscott TG, Eldridge GA, Franz PR (1988) A survey of the incidence of high pH beef meat in Victorian abattoirs. International Congress of Meat Science and Technology, Brisbane, Australia 34, 150–151.

Warner RD, Kauffman RG, Russell RL (1993) Quality attributes of major porcine muscles: a comparison with the longissimus lumborum. Meat Science 33, 359–372.
Quality attributes of major porcine muscles: a comparison with the longissimus lumborum.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3Mbntlamtw%3D%3D&md5=12538503e2de6c7eebbe5bcf3c2ea6c7CAS | 22060153PubMed |

Warner RD, Ferguson DM, McDonagh MB, Channon HA, Cottrell JJ, Dunshea FR (2005) Acute exercise stress and electrical stimulation influence the consumer perception of sheep meat eating quality and objective quality traits. Australian Journal of Experimental Agriculture 45, 553–560.
Acute exercise stress and electrical stimulation influence the consumer perception of sheep meat eating quality and objective quality traits.Crossref | GoogleScholarGoogle Scholar |

Warner RD, Dunshea FR, Ponnampalam EN, Gardner GE, Martin KM, Salvatore L, Hopkins DL, Pethick DW (2006) Quality meat from merinos. International Journal of Sheep and Wool Science 54, 48–53.

Warner RD, Ponnampalam EN, Kearney GA, Hopkins DL, Jacob RH (2007) Genotype and age at slaughter influence the retail shelf-life of the loin and knuckle from sheep carcasses. Australian Journal of Experimental Agriculture 47, 1190–1200.
Genotype and age at slaughter influence the retail shelf-life of the loin and knuckle from sheep carcasses.Crossref | GoogleScholarGoogle Scholar |

Warner RD, Jacob RH, Hocking Edwards JEH, McDonagh M, Pearce K, Geesink G, Kearney G, Allingham P, Hopkins DL, Pethick DW (2010) Quality of lamb meat from the Information Nucleus Flock. Animal Production Science 50, 1123–1134.
Quality of lamb meat from the Information Nucleus Flock.Crossref | GoogleScholarGoogle Scholar |

Warner RD, Gutzke D, Lau J, Kearney GA (2014) Factors influencing the incidence of heat-toughening in beef carcasses in Australia. Animal Production Science 54, 363–374.
Factors influencing the incidence of heat-toughening in beef carcasses in Australia.Crossref | GoogleScholarGoogle Scholar |

Wythes JR, Shorthose WR (1984) ‘Marketing cattle: its effect on liveweight, carcasses and meat quality. AMRC review.’ (AMRC: Sydney)