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RESEARCH ARTICLE
Contents Vol 50(9)

Effects of dietary protein level and zinc oxide supplementation on performance responses and gastrointestinal tract characteristics in weaner pigs challenged with an enterotoxigenic strain of Escherichia coli

J. M. Heo A , J. C. Kim B , C. F. Hansen A C , B. P. Mullan B , D. J. Hampson A and J. R. Pluske A D
+ Author Affiliations
- Author Affiliations

A Animal Research Institute, School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA 6150, Australia.

B Animal Research and Development, Department of Agriculture and Food, Locked Bag Number 4, Bentley Delivery Centre, WA 6983, Australia.

C Department of Large Animal Sciences, Faculty of Life Sciences, University of Copenhagen, Groennegaardsvej 2, DK-1870 Frederiksberg C, Denmark.

D Corresponding author. Email: j.pluske@murdoch.edu.au

Animal Production Science 50(9) 827-836 https://doi.org/10.1071/AN10058
Submitted: 6 April 2010  Accepted: 30 June 2010   Published: 29 September 2010

Abstract

The interactive effects of dietary protein level, zinc oxide (ZnO) supplementation and infection with an enterotoxigenic strain of Escherichia coli (ETEC) on performance responses and gastrointestinal tract characteristics were examined. Ninety-six individually housed, 21-day-old pigs (1 : 1 gender ratio) with initial bodyweight (BW) of 7.2 ± 0.69 kg, were used in a split plot experiment, with the whole plot being challenge or no challenge with ETEC and the dietary treatments used as subplots and arranged in a completely randomised 2 × 2 factorial design, with the factors being (i) two dietary protein levels (251 versus 192 g/kg crude protein) and (ii) addition or no addition of 2.5 g/kg ZnO. No antibiotic was added to the diet. The ETEC infection decreased average daily gain (P < 0.001) and increased feed conversion ratio (P < 0.01). Protein level had no effect on performance of pigs while ZnO supplementation increased (P < 0.001) average daily gain and average daily feed intake and hence decreased feed conversion ratio (P < 0.001). There were no 2- or 3-way interactions for growth performance indices (P > 0.05). Feeding a lower protein diet did not influence (P > 0.05) faecal volatile fatty acid concentrations. In non-infected pigs, feeding a lower protein diet caused a lower pH in the jejunum and ileum compared with pigs fed a higher protein diet (P < 0.05 and P < 0.01, respectively). However, feeding ZnO-supplemented diets increased (P < 0.05) the pH in the stomach and caecum compared with feeding diets without ZnO supplementation. Protein level did not alter (P > 0.05) empty BW but dietary supplementation with ZnO increased empty BW (P < 0.05). Neither protein level nor ZnO supplementation modified small intestinal morphology, although a tendency for an interaction (P < 0.1) was detected for jejunal villous height between protein level and ZnO supplementation. The results indicate that feeding ZnO-supplemented diets improved pig performance, and feeding a lower protein diet without ZnO supplementation did not compromise performance nor modify measures of gastrointestinal tract structure and function compared with pigs fed a diet higher in protein after weaning.

Additional keywords: growth performance, post-weaning diarrhoea.


Acknowledgements

The authors thank Mr Bob Davis, Mr Richard Seaward, Ms Josie Mansfield, Ms Aracely Hernandez and Ms Danka Halas for technical assistance. This research was supported by an Australian Research Council-Linkage Grant, with Danish Pig Production, the Department of Agriculture and Food of Western Australia, and Wandalup Farms Ltd, as the industry partners. Evonik Degussa kindly donated the isoleucine and valine.


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