Effects of fibrolytic enzymes and isobutyrate on ruminal fermentation, microbial enzyme activity and cellulolytic bacteria in pre- and post-weaning dairy calves
C. Wang A , Q. Liu A B , G. Guo A , W. J. Huo A , Y. X. Wang A , Y. L. Zhang A , C. X. Pei A and S. L. Zhang A
+ Author Affiliations
- Author Affiliations
A College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, P. R. China.
B Corresponding author. Email: liuqiangabc@163.com
Animal Production Science 59(3) 471-478 https://doi.org/10.1071/AN17270
Submitted: 1 May 2017 Accepted: 4 December 2017 Published: 7 February 2018
Abstract
The objective of the present study was to evaluate the effects of fibrolytic enzymes (FE, containing 160 units of cellulase and 4000 units of xylanase) or isobutyrate (IB) supplementation on ruminal fermentation, microbial enzyme activity and cellulolytic bacteria in dairy calves. Forty-eight Holstein bull calves of 15 days of age and of 44.9 ± 0.28 kg of BW were randomly assigned to four groups in a 2 × 2 factorial arrangement. Two levels of FE (0 g (FE–) or 1.83 g per calf per day (FE+)) and IB (0 g (IB–) or 6 g per calf per day (IB+)) were added. Calves were weaned at 60-day-old and four calves were selected from each treatment at random and slaughtered at 45 and 90 days of age. There was no IB × FE interaction effect. Ruminal pH decreased with IB or FE supplementation for post-weaned calves, whereas concentrations of total volatile fatty acids and acetate increased with IB or FE supplementation for pre- and post-weaned calves. Acetate to propionate ratio increased with IB supplementation, but was unaffected by FE supplementation. Ammonia-N concentration decreased with IB or FE supplementation for pre- and post-weaned calves. For post-weaned calves, activities of CMCase increased with IB or FE supplementation, and activities of cellobiase, xylanase, pectinase, β-amylase and protease increased with IB supplementation. Populations of B. fibrisolvens and F. succinogenes for pre- and post-weaned calves and R. flavefaciens for post-weaned calves increased with IB or FE supplementation. It is suggested that ruminal fermentation and growth performance of calves was improved with IB and FE supplementation, and the combination of IB and FE has the potential to stimulate the growth of pre- and post-weaned dairy calves.
Additional keywords: branched-chain volatile fatty acids, nutrient digestibility, ruminal bacteria, cellulase, xylanase.
References
Agarwal N, Kamra DN, Chaudhary LC, Agarwal I, Sahoo A, Pathak NN (2002) Microbial status and rumen enzyme profile of crossbred calves fed on different microbial feed additives. Letters in Applied Microbiology 34, 329–336.| Microbial status and rumen enzyme profile of crossbred calves fed on different microbial feed additives.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XksVSrs7s%3D&md5=142398ee0ee5f212c2e18764d7de28cfCAS |
Allen MS (2000) Effects of diet on short-term regulation of feed intake by lactating dairy cattle. Journal of Dairy Science 83, 1598–1624.
| Effects of diet on short-term regulation of feed intake by lactating dairy cattle.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXltVGgtb0%3D&md5=8bdd169ab9e9cf87c527ff4bd1f6a249CAS |
AOAC (1997) ‘Official methods of analysis.’ 16th edn. (Association of Official Analytical Chemists International: Gaithersburg, MD)
Arce-Cervantes O, Mendoza GD, Hernandez PA, Meneses M, Torres-Salado N, Loera O (2013) The effects of a lignocellulolytic extract of Fomes sp. EUM1 on the intake, digestibility, feed efficiency and growth of lambs. Animal Nutrition and Feed Technology 13, 363–372.
Balci F, Dikmen S, Gencoglu H, Orman A, Turkmen II, Birick H (2007) The effect of fibrolytic exogenous enzyme on fattening performance of steers. Bulgarian Journal of Veterinary Medicine 10, 113–118.
Beauchemin KA, Colombatto D, Morgavi DP, Yang WZ (2003) Use of exogenous fibrolytic enzymes to improve feed utilization by ruminants. Journal of Animal Science 81, E37–E47.
Bhasker TV, Nagalakshmi D, Rao DS (2013) Development of appropriate fibrolytic enzyme combination for maize stover and its effect on rumen fermentation in sheep. Asian-Australasian Journal of Animal Sciences 26, 945–951.
| Development of appropriate fibrolytic enzyme combination for maize stover and its effect on rumen fermentation in sheep.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhsFeqsL7F&md5=8c942ff8be30d7994741d4f23646e79bCAS |
Chung YH, Zhou M, Holtshausen L, Alexander TW, McAllister TA, Guan LL, Oba M, Beauchemin KA (2012) A fibrolytic enzyme additive for lactating Holstein cow diets: ruminal fermentation, rumen microbial populations and enteric methane emissions. Journal of Dairy Science 95, 1419–1427.
| A fibrolytic enzyme additive for lactating Holstein cow diets: ruminal fermentation, rumen microbial populations and enteric methane emissions.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XjtFWgtrY%3D&md5=2902d542e87466a778a09b81609b03f4CAS |
Cummins KA, Papas AH (1985) Effect of isocarbon 4 and isocarbon 5 volatile fatty acids on microbial protein synthesis and dry matter digestibility in vitro. Journal of Dairy Science 68, 2588–2595.
| Effect of isocarbon 4 and isocarbon 5 volatile fatty acids on microbial protein synthesis and dry matter digestibility in vitro.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2MXmtFeks7k%3D&md5=3536157b53b5fd08bc4b6af77e696776CAS |
Denman SE, McSweeney CS (2006) Developmentofa real-time PCR assay formonitoring anaerobic fungal and cellulolytic bacterial populations within the rumen. FEMS Microbiology Ecology 58, 572–582.
| Developmentofa real-time PCR assay formonitoring anaerobic fungal and cellulolytic bacterial populations within the rumen.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhtlSmtLrO&md5=5a7bb61378ac9e4e501af440c0f46b0dCAS |
Firkins JL, Yu Z, Morrison M (2007) Ruminal nitrogen metabolism: perspectives for integration of microbiology and nutrition for dairy. Journal of Dairy Science 90, E1–E16.
| Ruminal nitrogen metabolism: perspectives for integration of microbiology and nutrition for dairy.Crossref | GoogleScholarGoogle Scholar |
Forster RJ, Teather RM, Gong J, Deng SJ (1996) 16s rDNA analysis of Butyrivibrio fibrisolvens: phylogenetic position and relation to butyrate-producing anaerobic bacteria from the rumen of white-tailed deer. Letters in Applied Microbiology 23, 218–222.
| 16s rDNA analysis of Butyrivibrio fibrisolvens: phylogenetic position and relation to butyrate-producing anaerobic bacteria from the rumen of white-tailed deer.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28XmvFylsbY%3D&md5=59bf7130f756da2183dd2fe370b211c4CAS |
Gómez-Vazquez A, Mendoza GD, Aranda E, Perez J, Hernandez A, Pinos-Rodriguez JM (2011) Influence of fibrolytic enzymes on growth performance and digestion in steers grazing stargrass and supplemented with fermented sugarcane. Journal of Applied Animal Research 39, 77–79.
| Influence of fibrolytic enzymes on growth performance and digestion in steers grazing stargrass and supplemented with fermented sugarcane.Crossref | GoogleScholarGoogle Scholar |
Koike S, Kobayashi Y (2001) Development and use of competitive PCR assays for the rumen cellulolytic bacteria: Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus favefaciens. FEMS Microbiology Letters 204, 361–366.
| Development and use of competitive PCR assays for the rumen cellulolytic bacteria: Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus favefaciens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXos1Kiur8%3D&md5=87914eb0d6e8be03255f34582330803fCAS |
Kolver ES, DeVeth MJ (2002) Prediction of ruminal pH from pasture-based diets. Journal of Dairy Science 85, 1255–1266.
| Prediction of ruminal pH from pasture-based diets.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XktFWjs74%3D&md5=e8e35a3130d6c620e689ae3e1b2c6bccCAS |
Liu Q, Wang C, Huang YX, Dong KH, Yang WZ, Wang H (2008) Effects of isobutyrate on rumen fermentation, urinary excretion of purine derivatives and digestibility in steers. Archives of Animal Nutrition 62, 377–388.
| Effects of isobutyrate on rumen fermentation, urinary excretion of purine derivatives and digestibility in steers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhtVOrurvI&md5=292f6657892495b593a064fc0bc82739CAS |
Liu Q, Wang C, Yang WZ, Zhang B, Yang XM, He DC, Dong KH, Huang YX (2009) Effects of isobutyrate on rumen fermentation, lactation performance and plasma characteristics in dairy cows. Animal Feed Science and Technology 154, 58–67.
| Effects of isobutyrate on rumen fermentation, lactation performance and plasma characteristics in dairy cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXht1aisLbI&md5=58be0abbd252c6134821e7f0bcfc4af5CAS |
Liu Q, Wang C, Pei CX, Li HY, Wang YX, Zhang SL, Zhang YL, He JP, Wang H, Yang WZ, Bai YS, Shi ZG, Liu XN (2014) Effects of isovalerate supplementation on microbial status and rumen enzyme profile in steers fed on corn stover based diet. Livestock Science 161, 60–68.
| Effects of isovalerate supplementation on microbial status and rumen enzyme profile in steers fed on corn stover based diet.Crossref | GoogleScholarGoogle Scholar |
Liu Q, Wang C, Zhang YL, Pei CX, Zhang SL, Wang YX, Zhang ZW, Yang WZ, Wang H, Guo G, Huo WJ (2016a) Effects of isovalerate supplementation on growth performance and ruminal fermentation in pre- and post-weaning dairy calves. The Journal of Agricultural Science 154, 1499–1508.
| Effects of isovalerate supplementation on growth performance and ruminal fermentation in pre- and post-weaning dairy calves.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC28Xhs1eltrzF&md5=2d796cca90e76b34a2e76a7ab7978e5aCAS |
Liu Q, Wang C, Zhang YL, Pei CX, Zhang SL, Li HQ, Guo G, Huo YJ, Yang WZ, Wang H (2016b) Effects of 2-methybutyrate supplementation on growth performance and ruminal development in pre- and post-weaning dairy calves. Animal Feed Science and Technology 216, 129–137.
| Effects of 2-methybutyrate supplementation on growth performance and ruminal development in pre- and post-weaning dairy calves.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC28XltF2qtb8%3D&md5=f1cdffbffa6c62de5ad80c19507fa4f8CAS |
Mao HL, Wu CH, Wang JK, Liu JX (2013) Synergistic effect of cellulase and xylanase on in vitro rumen fermentation and microbial population with rice straw as substrate. Animal Nutrition and Feed Technology 13, 477–487.
Mouriño F, Akkarawongsa RA, Weimer PJ (2001) Initial pH as a determinant of cellulose digestion rate by mixed ruminal microorganisms in vitro. Journal of Dairy Science 84, 848–859.
| Initial pH as a determinant of cellulose digestion rate by mixed ruminal microorganisms in vitro.Crossref | GoogleScholarGoogle Scholar |
NRC (2001) ‘National Research Council. Nutrient requirements of dairy cattle. Chapter 10: nutrient requirements of young calf.’ 7th rev. edn. (Ed. Subcommittee on Dairy Cattle Nutrition, Committee on Animal Nutrition, and Board on Agriculture and Natural Resources) pp. 214–233. (National Academy of Sciences: Washington, DC)
Nsereko VL, Beauchemin KA, Morgavi DP, Rode LM, Furtado AF, McAllister TA, Iwaasa AD, Yang WZ, Wang Y (2002) Effect of a fibrolytic enzymes preparation from Trichoderma longibrachiatum on the rumen microbial population of dairy cows. Canadian Journal of Microbiology 48, 14–20.
| Effect of a fibrolytic enzymes preparation from Trichoderma longibrachiatum on the rumen microbial population of dairy cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XitF2mu70%3D&md5=ad4dfebaf4d776c839fb96c29b559e06CAS |
Pei CX, Liu Q, Dong CS, Li HQ, Jiang JB, Gao WJ (2013) Microbial community in the forestomachs of alpacas (Lama pacos) and sheep (Ovis aries). Journal of Integrative Agriculture 12, 314–318.
| Microbial community in the forestomachs of alpacas (Lama pacos) and sheep (Ovis aries).Crossref | GoogleScholarGoogle Scholar |
Pinos-Rodríguez JM, González SS, Mendoza GD, Bárcena R, Cobos MA, Hernández A, Ortega ME (2002) Effects of exogenous fibrolytic enzyme on ruminal fermentation and digestibility of alfalfa and ryegrass hay fed to lambs. Journal of Animal Science 80, 3016–3020.
| Effects of exogenous fibrolytic enzyme on ruminal fermentation and digestibility of alfalfa and ryegrass hay fed to lambs.Crossref | GoogleScholarGoogle Scholar |
Romero JJ, Zarate MA, Queiroz OCM, Han JH, Shin JH, Staples CR, Brown WF, Adesogan AT (2013) Fibrolytic enzymes and ammonia application effects on the nutritive value, intake, and digestion kinetics of bermudagrass hay in beef cattle. Journal of Animal Science 91, 4345–4356.
| Fibrolytic enzymes and ammonia application effects on the nutritive value, intake, and digestion kinetics of bermudagrass hay in beef cattle.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhsVOlsbvE&md5=64449c1cadd9a14b26622c4c888599abCAS |
SAS (2002) ‘User’s guide: statistics.’ Version 9. (SAS Institute Inc.: Cary, NC)
Silva TH, Takiya CS, Vendramini TH, De Jesus EF, Zanferari F, Rennó FP (2016) Effects of dietary fibrolytic enzymes on chewing time, ruminal fermentation, and performance of mid-lactating dairy cows. Animal Feed Science and Technology 221, 35–43.
| Effects of dietary fibrolytic enzymes on chewing time, ruminal fermentation, and performance of mid-lactating dairy cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC28XhsVeltbfM&md5=b07f7e2846474993b665cfe4dac60fd1CAS |
Titi HH, Tabbaa MJ (2004) Efficacy of exogenous cellulase on digestibility in lambs and growth of dairy calves. Livestock Production Science 87, 207–214.
| Efficacy of exogenous cellulase on digestibility in lambs and growth of dairy calves.Crossref | GoogleScholarGoogle Scholar |
Van Soest PJ, Robertson JB, Lewis BA (1991) Methods for dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.
| Methods for dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK38%2FnvVCltA%3D%3D&md5=1bbe217b400e9f7fff04f7f3e179f03aCAS |
Wang C, Liu Q, Zhang YL, Pei CX, Zhang SL, Wang YX, Yang WZ, Bai YS, Shi ZG, Liu XN (2015) Effects of isobutyrate supplementation on ruminal microflora, rumen enzyme activities and methane emissions in Simmental steers. Journal of Animal Physiology and Animal Nutrition 99, 123–131.
| Effects of isobutyrate supplementation on ruminal microflora, rumen enzyme activities and methane emissions in Simmental steers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXnslSisw%3D%3D&md5=5e4985e4afadbf769d3cee764866a8ccCAS |
Wang C, Liu Q, Zhang YL, Pei CX, Zhang SL, Guo G, Huo WJ, Yang WZ, Wang H (2017) Effects of isobutyrate supplementation in pre- and post-weaned dairy calves diet on growth performance, rumen development, blood metabolites and hormone secretion. Animal 11, 794–801.
| Effects of isobutyrate supplementation in pre- and post-weaned dairy calves diet on growth performance, rumen development, blood metabolites and hormone secretion.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2sXmtFCgtrc%3D&md5=3eb22d1cdee0a40114dbd98355270b63CAS |
Yang WZ, Beauchemin KA, Rode LM (2000) A comparison of methods of adding fibrolytic enzymes to lactating cow diets. Journal of Dairy Science 83, 2512–2520.
| A comparison of methods of adding fibrolytic enzymes to lactating cow diets.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXosVCgsLc%3D&md5=12f39b056dc5391125ef9fb75126c763CAS |
Yu Z, Morrison M (2004) Improved extraction of PCR-quality community DNA from digesta and fecal sample. BioTechniques 36, 808–812.
