Lack of response to garlic fed at different dose rates for the control of Haemonchus contortus in Merino wether lambs
V. J. Strickland A B C , J. S. Fisher A , W. T. Potts B and G. W. Hepworth AA School of Agriculture and Environment, Curtin University of Technology, PMB 1, Northam, WA 6401, Australia.
B Specialty Feeds Pty Ltd, 3150 Great Eastern Highway, Glen Forrest, WA 6071, Australia.
C Corresponding author. Email: victoria.strickland@postgrad.curtin.edu.au
Animal Production Science 49(12) 1093-1099 https://doi.org/10.1071/AN09057
Submitted: 7 April 2009 Accepted: 4 July 2009 Published: 16 November 2009
Abstract
With the increased incidence of parasite resistance to chemical anthelmintics worldwide novel approaches to manage parasite infection, such as medicinal plants and their extracts, are being investigated by the scientific community. The current study tested the effect of three rates of garlic (0.9, 1.8 and 3.6%) in a pelleted ration on Haemonchus contortus in sheep. Thirty-nine Merino wether lambs aged 6 months were divided into five treatment groups, including three garlic dose rates and two control groups that received no garlic. All animals were infected with 4000 L3 H. contortus larvae 3 weeks after allocation to treatments. A positive control group was drenched with abamectin 28 days after infection. The synthetic drench was effective in controlling the parasites, but there was no reduction in either worm egg counts (WEC) or total worm count due to the garlic. The 3.6% garlic treatment had significantly lower (P < 0.05) liveweight, feed intake, body condition score and feed conversion ratio than any of the other treatment groups, suggesting that this level of garlic had a low level of anti-nutritional properties. There was an interaction between faecal WEC and voluntary feed intake over time, with the animals with higher voluntary feed intake having lower WEC over time.
Additional keywords: gastrointestinal nematodes, sheep.
Acknowledgements
The authors would like to acknowledge Drs Deiter Palmer and Gaye Krebs for their helpful comments on the design of the experiment. We would like to thank the animal biology group at The University of Western Australia for making the facility at Allandale Farm available for the experiment and the staff at Allandale farm for assistance in carrying out the work; particularly Steve Gray, Phillipa Gray and John Beesley. We also thank Dr Malcolm Knox from CSIRO in Armidale, NSW, for growing and supplying the H. contortus larvae, and the staff at Tammin Abattoirs for assisting with the collection of the digestive tracts. Thanks also to Speciality Feeds for the manufacture of the diets, Australian Garlic Producers for supplying the garlic and ATA Engineering for their support.
Alawa CBI,
Adamu AM,
Gefu JO,
Ajanusi OJ,
Abdu PA,
Chiezey NP,
Alawa JN, Bowman DD
(2003) In vitro screening of two Nigerian medicinal plants (Vernonia amygdalina and Annona senesalensis) for anthelmintic activity. Veterinary Parasitology 113, 73–81.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
Athanasiadou S, Kyriazakis I
(2004) Plant secondary metabolite: antiparasitic effects and their role in ruminant production systems. The Proceedings of the Nutrition Society 63, 631–639.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
Athanasiadou S,
Tzamaloukas O,
Kyriazakis I,
Jackson F, Coop RL
(2005) Testing for direct anthelmintic effects of bioactive forages against Trichostrongylus colubriformus in grazing sheep. Veterinary Parasitology 127, 233–243.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
Athanasiadou S,
Githori J, Kyriazakis I
(2007) Medical plants for helminth parasite control: facts and fiction. Animal 1, 1392–1400.
| Crossref | GoogleScholarGoogle Scholar |
Besier RB
(2009) A novel anthelmintic group: welcome relief for sheep farmers. New Zealand Veterinary Journal 57, 1–2.
| PubMed |
Besier RB, Love SCJ
(2003) Anthelmintic resistance in sheep nematodes in Australia: the need for new approaches. Australian Journal of Experimental Agriculture 43, 1383–1391.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
Bricarello PA,
Amarante AFT,
Rocha RA,
Cabral Filho SL,
Huntlry JF,
Houdijk JGM,
Abdalla AL, Gennari SM
(2005) Influence of dietary protein supply on resistance to experimental infections with Haemonchus contortus in Ile de France and Santa Ines lambs. Veterinary Parasitology 134, 99–109.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
Burke JM,
Wells A,
Casey P, Miller JE
(2009) Garlic and papaya lack of control over gastrointestinal nematodes in goats and lambs. Veterinary Parasitology 159, 171–174.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
Eady SD,
Woolaston RR, Barger IA
(2003) Comparison of genetic and nongenetic strategies for control of gastrointestinal nematodes of sheep. Livestock Production Science 81, 11–23.
| Crossref | GoogleScholarGoogle Scholar |
Githiori JB,
Hoglund J,
Waller PJ, Barker RL
(2002) Anthelmintic activity of preparations derived from Myrsine africana and Rapanea melanophloeos against the nematode parasite, Haemonchus contortus, of sheep. Journal of Ethnopharmacology 80, 187–191.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Githiori JB,
Hoglund J,
Waller PJ, Barker RL
(2003) Evaluation of anthelmintic properties of extracts from some plants used as livestock dewormers by pastoralists and smallholder farmers in Kenya against Heligmosomoides polygyrus infections in mice. Veterinary Parasitology 118, 215–226.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Githiori JB,
Athanasiadou S, Thamsborg SM
(2006) Use of plants in novel approaches for control of gastrointestinal helminths in livestock with emphasis on small ruminants. Veterinary Parasitology 139, 308–320.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Hordegen P,
Hertzberg H,
Heilmann J,
Langhans W, Maurer V
(2003) The anthelmintic efficacy of five plants products against gastrointestinal trichostrongylids in artificially infected lambs. Veterinary Parasitology 117, 51–60.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
Jefferies BC
(1961) Body condition scoring and its use in management. The Tasmanian Journal of Agriculture 32, 19–21.
Kahiya C,
Mukaratirwa S, Thamsborg SM
(2003) Effects of Acacia nilotica and Acacia karoo diets on Haemonchus contortus infection in goats. Veterinary Parasitology 115, 265–274.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
Kaminsky R,
Gauvry N,
Schorderet Weber S,
Skripsky T, Bouvier J , et al.
(2008) Identification of the amino-acetonitrile derivative monepantel (ADD1566) as a new anthelmintic drug development candidate. Parasitology Research 103, 931–939.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
Kaplan RM,
Vidyashankar AN,
Howell SB,
Neiss JM,
Williamson LH, Terrill TH
(2007) A novel approach for combining the use of in vitro and in vivo data to measure and detect emerging moxidectin resistance in gastrointestinal nematodes of goats. International Journal for Parasitology 37, 795–804.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
Karlsson LJE, Greeff JC
(2006) Selection response in faecal worm egg counts in the Rylington Merion parasite resistant flock. Australian Journal of Experimental Agriculture 46, 809–811.
| Crossref | GoogleScholarGoogle Scholar |
Ketzis JK,
Taylor A,
Bowman DD,
Brown DL,
Warnick LD, Erb HN
(2002) Chenopodium ambrosioides and its essential oil as treatments for Haemonchus contortus and mixed adult-nematode infections in goats. Small Ruminant Research 44, 193–200.
| Crossref | GoogleScholarGoogle Scholar |
Maciel MV,
Morais SM,
Bevilaqua CML,
Camurca-Vasconcelos ALF,
Costa CTC, Castro CMS
(2006) Ovicidal and larvicidal activity of Melia azedarach extracts on Haemonchus contortus. Veterinary Parasitology 140, 98–104.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
Molan AL,
Duncan AJ,
Barry NT, McNabb WC
(2003) Effects of condensed tannins and crude sesquiterpene lactones extracted from chicory on the motility of larvae of deer lungworm and gastrointestinal nematodes. Parasitology International 52, 209–218.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
Nolte DL, Provenza FD
(1992a) Food preferences in lambs after exposure to flavours in milk. Applied Animal Behaviour Science 32, 381–389.
| Crossref | GoogleScholarGoogle Scholar |
Nolte DL, Provenza FD
(1992b) Food preferences in lambs after exposure to flavours in solid foods. Applied Animal Behaviour Science 32, 337–347.
| Crossref | GoogleScholarGoogle Scholar |
Pena N,
Auro A, Sumano H
(1988) A comparative trial of garlic, its extract and ammonium potassium tartrate as anthelmintics in carp. Journal of Ethnopharmacology 24, 199–203.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
Robertson E,
Gordon IJ, Perez-Barberia FJ
(2006) Preferences of sheep and goats for straw pellets treated with different food-flavouring agents. Small Ruminant Research 63, 50–57.
| Crossref | GoogleScholarGoogle Scholar |
Romani R,
Ieri F,
Turchetti B,
Mulinacci N,
Vincieri FF, Buzzini P
(2006) Analysis of condensed and hydrolysable tannins from commercial plant extracts. Journal of Pharmaceutical and Biomedical Analysis 5615, 1–6.
Steel JW
(2003) Effects of protein supplementation of young sheep on resistance development and resilience to parasitic nematodes. Australian Journal of Experimental Agriculture 43, 1469–1476.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
Strain SAJ, Stear MJ
(2001) The influence of protein supplementation on the immune response to Haemonchus contortus. Parasite Immunology 23, 527–531.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
Strickland VJ,
Krebs GL, Potts W
(2009) Pumpkin kernel and garlic as alternative treatments for the control of Haemonchus contortus in sheep. Animal Production Science 49, 139–144.
| Crossref | GoogleScholarGoogle Scholar |
Torres-Acosta JFJ, Hoste H
(2008) Alternative or improved methods to limit gastro-intestinal parasitism in grazing sheep and goats. Small Ruminant Research 77, 159–173.
| Crossref | GoogleScholarGoogle Scholar |
Waller PJ
(1999) International approaches to the concept of integrated control of nematode parasites of livestock. International Journal for Parasitology 29, 155–164.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
Wood IB,
Amaral NK,
Bairded K,
Duncan JL, Kassai T , et al.
(1995) World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) second edition guidelines for evaluating the efficacy of anthelmintics in ruminants (bovine, ovine, caprine). Veterinary Parasitology 58, 181–213.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
