Effect of inbreeding depression on bull sperm quality and field fertility
Jesús Dorado A , Rosa Morales Cid B , Antonio Molina B , Manuel Hidalgo A , Julia Ariza C , Miguel Moreno-Millán B and Sebastián Demyda-Peyrás D E
+ Author Affiliations
- Author Affiliations
A Department of Animal Medicine and Surgery, University of Cordoba, CN IV KM 396 – Campus Rabanales, Fco. Santisteban building, CP 14071, Cordoba, Spain.
B Department of Genetics, University of Cordoba, CN IV KM 396 – Campus Rabanales, Gregor Mendel Building, CP 14071, Cordoba, Spain.
C Department of Cell Biology, Physiology and Immunology, University of Cordoba, CN IV KM 396 – Campus Rabanales. Gregor Mendel Building. CP 14071. Cordoba, Spain.
D Instituto de Genetica Veterinaria (IGEVET), CCT La Plata, CONICET – Facultad de Ciencias Veterinarias – Universidad de La Plata, Buenos Aires, Argentina.
E Corresponding author. Email: sebass@uco.es
Reproduction, Fertility and Development 29(4) 712-720 https://doi.org/10.1071/RD15324
Submitted: 7 August 2015 Accepted: 9 November 2015 Published: 18 December 2015
Abstract
The present study investigated the effect of inbreeding depression on sperm quality using automated and objective methods and subsequent effects on beef bull field fertility. Individual inbreeding coefficient (F) values and field fertility data were determined using a dataset of AI bulls belonging to the Spanish Retinta Breeders Association (Asociación Nacional de Criadores de Ganado Vacuno Selecto de Raza Retinta (ANCRE)). Animals were clustered in two groups according to the F values as follows: (1) a high inbreeding group (HI; F ≥ 13.5%, mean 16.3); and (2) a non-inbreeding group (NI; F = 0%). In total, 17 different assessments were performed in both experimental groups, including evaluation of sperm morphology, acrosomal and DNA status, sperm plasma membrane integrity and function (hypo-osmotic swelling test), 10 kinetic parameters and the structure of sperm subpopulations. Sperm morphology, acrosomal and DNA status and osmotic tolerance were similar in both groups. Three velocity parameters (curvilinear velocity, straight line velocity and average path velocity) and the amplitude of lateral head displacement were higher in HI (P < 0.05). Cluster analysis of kinematic parameters revealed three different sperm subpopulations (sP1, sP2 and sP3), with the proportion of the sP1 population (highly active but non-progressive spermatozoa) being significantly (P < 0.05) higher in the HI group. Field fertility was assessed using two calving record datasets. In a smaller database including only bulls evaluated in the present study, there was a significant increase in the calving interval of cows sired with HI bulls. Conversely, in an extended genetic analysis of the ANCRE database, inbreeding only explained a small part of the variation in calving interval, and the results of regression analysis were not significant among bulls. The findings of the present study suggest that high inbreeding levels have a moderate effect on bull semen quality, with an increased percentage of highly active but non-progressive spermatozoa, but only when F values reached a certain threshold. This motility pattern could explain, in part, the higher calving interval produced by inbred bulls under field conditions.
Additional keywords: calving interval, cattle, computer-aided sperm analysis (CASA), DNA fragmentation, genetic trait, sperm function, sperm morphology, sperm subpopulations.
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