Mouse oocyte meiosis is disturbed by knockdown of Suv4-20h
Kai Xiong A B , Wei Wu A B , Xuguang Wang A , Xueshan Ma A , Jie Chen A and Honglin Liu A C
+ Author Affiliations
- Author Affiliations
A College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
B These authors contributed equally to this study.
C Corresponding author. Email: liuhonglin@263.net
Reproduction, Fertility and Development 25(3) 503-510 https://doi.org/10.1071/RD12116
Submitted: 12 April 2012 Accepted: 14 June 2012 Published: 10 August 2012
Abstract
Suv4-20h was initially characterised as a histone methyltransferase (HMTase) that catalyses lysine 20 of histone H4 dimethylation (H4K20me2) and trimethylation (H4K20me3). In the present study, using RNA interference (RNAi), we found that Suv4-20h activity is required for the fidelity of chromosome distribution during meiosis in the mammalian oocyte. Knockdown of Suv4-20h resulted in attenuation of H4K20me3 and the accumulation of H4K20me1. After Suv4-20h knockdown, oocytes exhibited an increasing percentage of aberrant chromosome alignment in MI, together with a decreasing percentage of polar body I extrusion. We conclude that Suv4-20h may be required for normal chromosome behaviour and that it is crucial for proper meiotic progression in mammalian oocytes.
Additional keywords: epigenetics, histone modification.
References
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