Knockdown of UCHL5IP causes abnormalities in γ-tubulin localisation, spindle organisation and chromosome alignment in mouse oocyte meiotic maturation
Ya-Peng Wang A B , Shu-Tao Qi A B , Yanchang Wei A , Zhao-Jia Ge A B , Lei Chen A B , Yi Hou A , Ying-Chun Ouyang A , Heide Schatten C , Jian-Guo Zhao A D and Qing-Yuan Sun A D
+ Author Affiliations
- Author Affiliations
A State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
B Graduate School, Chinese Academy of Sciences, Beijing, 100101, China.
C Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65203, USA.
D Corresponding authors. Emails: zhaojg@ioz.ac.cn; sunqy@ioz.ac.cn
Reproduction, Fertility and Development 25(3) 495-502 https://doi.org/10.1071/RD12300
Submitted: 20 April 2012 Accepted: 16 October 2012 Published: 27 November 2012
Abstract
UCHL5IP is one of the subunits of the haus complex, which is important for microtubule generation, spindle bipolarity and accurate chromosome segregation in Drosophila and human mitotic cells. In this study, the expression and localisation of UCHL5IP were explored, as well as its functions in mouse oocyte meiotic maturation. The results showed that the UCHL5IP protein level was consistent during oocyte maturation and it was localised to the meiotic spindle in MI and MII stages. Knockdown of UCHL5IP led to spindle defects, chromosome misalignment and disruption of γ-tubulin localisation in the spindle poles. These results suggest that UCHL5IP plays critical roles in spindle formation during mouse oocyte meiotic maturation.
Additional keywords: haus protein complex, meiosis.
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