Detecting co-cultivation induced chemical diversity via 2D NMR fingerprints
Larissa Buedenbender A C D , Anthony R Carroll A E and D İpek Kurtböke BA Griffith School of Environment, Griffith University, Gold Coast Campus, Qld 4222, Australia
B Genecology Research Centre, School of Science and Engineering, University of the Sunshine Coast, Maroochydore, Qld 4558, Australia. Tel: +61 7 5430 2819, Email: ikurtbok@usc.edu.au
C Present address: GEOMAR Centre for Marine Biotechnology (GEOMAR-Biotech), Research Unit Marine Natural Products Chemistry, GEOMAR Helmholtz Centre for Ocean Research Kiel, 24106 Kiel, Germany
D Tel: +49 431 600 4424, Email: larissa.buedenbender@alumni.griffithuni.edu.au
E Tel: +61 7 5552 9187, Email: a.carroll@griffith.edu.au
Microbiology Australia 40(4) 186-189 https://doi.org/10.1071/MA19054
Published: 11 November 2019
Abstract
Rediscovery of already known compounds is a major issue in microbial natural product drug discovery. In recent years, progress has been made in developing more efficient analytical approaches that quickly identify known compounds in a sample to minimise rediscovery. In parallel, whole genome sequencing of microorganisms has revealed their immense potential to produce secondary metabolites, yet the majority of biosynthetic genes remain silent under common laboratory culturing conditions. Therefore, increased research has focused on optimising culturing methods to activate the silent biosynthetic gene clusters. Co-cultivation of different microbial strains can activate biosynthetic gene clusters that remain silent under standard laboratory fermentations involving mono-cultures, hence, the technique has great potential for natural product drug discovery. However, innovative methods are still needed to evaluate the success of any co-cultured fermentation end-product. Here, the application of HSQC-TOCSY NMR spectra and subsequent PCoA to identify changes in the metabolite diversity induced through co-cultivation is described.
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