Arrays in clinical microbiology
Joanne MercerDepartment of Microbiology and Infectious Diseases
Sydney South West Pathology, Liverpool
Locked Bag 7090
Liverpool BC, NSW 1871, Australia
Tel: +61 2 9828 5124
Fax: +61 2 9828 5129
Email: joanne.mercer@sswahs.nsw.gov.au
Microbiology Australia 34(4) 184-185 https://doi.org/10.1071/MA13063
Published: 31 October 2013
Abstract
Application of molecular techniques in the diagnostic microbiology laboratory for the detection of pathogens continues to develop and expand. Simultaneous detection of large numbers of microbial targets or resistance genes with minimal hands-on time is a prominent objective of many new molecular systems. In addition to established real-time PCR detection, microarray technology or the newly developed FilmArray system (an automated nested PCR with melt curve analysis), are promising tools to aid infectious disease diagnosis.
References
[1] Miller, M. and Tang, Y. (2009) Basic concepts of microarrays and potential applications in clinical microbiology. Clin. Microbiol. Rev. 22, 611–633.| Basic concepts of microarrays and potential applications in clinical microbiology.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhsVyhsrfP&md5=405debb2d022fcb77ebabde0ac0d1325CAS | 19822891PubMed |
[2] Stover, A. et al. (2004) Hybridization array technology. In Molecular Microbiology, Diagnostic Principles and Practice (Persing, D., ed), 619–639, ASM Press.
[3] Pabbaraju, K. et al. (2008) Comparison of the Luminex xTAG respiratory viral panel with in-house nucleic acid amplification tests for diagnosis of respiratory virus infections. J. Clin. Microbiol. 46, 3056–3062.
| Comparison of the Luminex xTAG respiratory viral panel with in-house nucleic acid amplification tests for diagnosis of respiratory virus infections.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhtFOku7vK&md5=e79054dc843b70801d1a862f075e90ddCAS | 18632902PubMed |
[4] Poritz, M. et al. (2011) FilmArray, an automated nested multiplex PCR system for multi-pathogen detection: development and application to respiratory tract infection. PLoS ONE 6, e26047.
| FilmArray, an automated nested multiplex PCR system for multi-pathogen detection: development and application to respiratory tract infection.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhsVaksr3F&md5=7df20960d63e5632626717b2e6c4ff1bCAS | 22039434PubMed |
[5] Loeffelholz, M. et al. (2011) Comparison of the FilmArray respiratory panel and Prodesse real-time PCR assays for the detection of respiratory pathogens. J. Clin. Microbiol. 49, 4083–4088.
| Comparison of the FilmArray respiratory panel and Prodesse real-time PCR assays for the detection of respiratory pathogens.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC38%2FjtVGqug%3D%3D&md5=901169fe54ea48b11c9454652dd1ac01CAS | 21998418PubMed |
[6] Rand, K. et al. (2011) Comparison of two multiplex methods for detection of respiratory viruses: Filmarray RP and xTAG RVP. J. Clin. Microbiol. 49, 2449–2453.
| Comparison of two multiplex methods for detection of respiratory viruses: Filmarray RP and xTAG RVP.Crossref | GoogleScholarGoogle Scholar | 21508156PubMed |
