Genetic diversity of marine oligochaetous clitellates in selected areas of the South Atlantic as revealed by DNA barcoding
Alessandro L. Prantoni A D , Ricardo Belmonte-Lopes B , Paulo C. Lana A and Christer Erséus C
+ Author Affiliations
- Author Affiliations
A Center for Marine Studies, Federal University of Paraná, Av. Beira Mar, s/n, 83255-976 Pontal do Paraná, Paraná, Brazil.
B Department of Zoology, Laboratory of Evolutionary Dynamics and Complex Systems, Federal University of Paraná, Av. Francisco H. dos Santos, 100, 81531-980 Curitiba, Paraná, Brazil.
C Department of Biological and Environmental Sciences, University of Gothenburg, Box 463, SE-405 30 Göteborg, Sweden.
D Corresponding author. Email: aprantoni@gmail.com
Invertebrate Systematics 32(3) 524-532 https://doi.org/10.1071/IS17029
Submitted: 25 March 2017 Accepted: 9 September 2017 Published: 26 April 2018
Abstract
Marine oligochaetous clitellates are poorly investigated in the South Atlantic Ocean, especially along the east coast of South America. Closely related species are often difficult to distinguish based on morphology. The lack of specialists and modern identification guides have been pointed out as the main reasons for the scarcity of studies in the South Atlantic Ocean as a whole. To increase the knowledge of this group in the South Atlantic, the genetic diversity of a sample of marine oligochaetous clitellates from Brazil, South Africa and Antarctica was assessed by the Automatic Barcode Gap Discovery (ABGD) and the generalised mixed Yule coalescent (GMYC) approaches. In total, 80 cytochrome c oxidase subunit I (COI) sequences were obtained, each with ~658 bp, estimated to represent 32 distinct putative species. ABGD established a barcoding gap between 3% and 14% divergence for uncorrected p-distances and the estimates of GMYC were largely concordant. All the clusters or putative species were genetically associated with previously known species or genera. This study thus confirms the adequacy of the COI barcoding approach combined with a genetic divergence threshold at the order of 10% for marine oligochaetous clitellates.
Additional keywords: COI, DNA barcodes, marine clitellates, southern Atlantic Ocean, species delimitation.
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