Identification and expression characterisation of SbERECTA family genes in Sorghum bicolor
Jia Cheng Zheng
A B C , Jie Yu C B , Ting Liu C B , Xin Wang B , Qiu Wen Zhan B , Jie Qin Li B , Zhao Shi Xu A C and You Zhi Ma A C
+ Author Affiliations
- Author Affiliations
A Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 10081, P.R. China.
B College of Agronomy, Anhui Science and Technology University, Fengyang, Anhui, 233100, P.R. China.
C Corresponding authors. Email: xuzhaoshi@caas.cn; mayouzhi@caas.cn
Crop and Pasture Science 72(2) 125-135 https://doi.org/10.1071/CP20434
Submitted: 29 October 2020 Accepted: 7 January 2021 Published: 16 February 2021
Journal Compilation © CSIRO 2021 Open Access CC BY
Abstract
ERECTAs are receptor-like kinases that regulate plant biomass and stress resistance. In this study, the wheat (Triticum aestivum) TaERECTA gene was used as a probe to identify the SbERECTA family genes (SbERs) in the sorghum (Sorghum bicolor) genome, analyse their subcellular localisation and characterise their expression. Results showed that the two SbER members, SbER10 with three copies (SbER10_X1, SbER10_X2, and SbER10_X3) and SbER4 with two copies (SbER4_X1 and SbER4_X2), were found on chromosomes 10 and 4 of sorghum, respectively. SbER10 had the highest expression level in the pedicel tissue and showed a remarkable response under treatment with abscisic acid, brassinolide, gibberellin and indole-3-acetic acid. SbER10_X1, functioning on the cell membrane and chloroplast, exhibited abundant transcript in only a few sorghum varieties that are grown in mountainous areas and receive strong light, heat, and water supply. Expression of SbER10_X1 was significantly and positively correlated with plant biomass of 32 sorghum germplasm resources. These results indicate that SbER10 genes have an important regulatory role in sorghum growth, and increasing SbER10 transcription level offers a potential strategic target for breeding or biotechnological approaches to enhance sorghum biomass and environmental adaptability.
Keywords: correlation analysis, ERECTA family, expression characteristics, SbER, sorghum.
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