Free radical scavenging activities can mitigate the effect of water stress in chickpea
Davinder Kaur A , Satvir Kaur Grewal A C , Jagmeet Kaur B and Sarvjeet Singh B
+ Author Affiliations
- Author Affiliations
A Department of Biochemistry, Punjab Agricultural University, Ludhiana – 141 004, India.
B Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana – 141 004, India.
C Corresponding author. Email: satvir_pau@pau.edu
Crop and Pasture Science 68(6) 544-554 https://doi.org/10.1071/CP17022
Submitted: 16 January 2017 Accepted: 5 June 2017 Published: 11 July 2017
Abstract
To get a comprehensive view of drought tolerance mechanisms, the influence of water deficit stress on antioxidative capacity due to scavenging of free radicals and ability to maintain reduced cell state was investigated in roots, nodules, leaves, pod wall and seeds of two chickpea cultivars differing in rooting behaviour. ICC4958 (deep rooted) possessed better ability to combat water deficit-induced oxidative stress relative to ILC3279 (shallow rooted) as revealed by increase in total phenol, reducing power, ferric reducing ability and capacity to scavenge 2,2-Diphenyl-1-picryl hydrazyl (DPPH) and OH free radicals. Effect of water deficit stress on photosynthetic pigments of these cultivars was also studied. The investigation revealed that the influence of water stress in enhancing antioxidative capacity was most prominent in roots of ICC4958 among all other tissues as revealed by increased total phenols, DPPH and OH free radical scavenging activity and total reducing power under stress. However, roots of ILC3279 suffered a decrease in total phenolic content, total reducing power and DPPH free radical scavenging activity under prolonged stress, which was reflected in reduced antioxidative defence in reproductive tissues like decreased reducing power in pod wall and ferric-reducing antioxidant power ability in seeds.
Additional keywords: antioxidant, reducing power, water deficit stress.
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