Differential performance of two forage species, Medicago truncatula and Sulla carnosa, under water-deficit stress and recovery
Aida Rouached A B , Inès Slama A C , Walid Zorrig A , Asma Jdey A , Caroline Cukier B , Mokded Rabhi A , Ons Talbi A , Anis Mohamed Limami B and Chedly Abdelly A
+ Author Affiliations
- Author Affiliations
A Laboratoire des Plantes Extrêmophiles, BP 901, Centre de Biotechnologie de Borj Cédria, Hammam-Lif 2050, Tunisia.
B University of Angers, UMR 1345 Research Institute of Horticulture and Seeds (INRA, Agrocampus-Ouest, University of Angers), SFR 4207 Quasav, 2 Bd Lavoisier, 49045 Angers Cedex, France.
C Corresponding author. Email: slama_ines@hotmail.fr
Crop and Pasture Science 64(3) 254-264 https://doi.org/10.1071/CP13049
Submitted: 6 February 2013 Accepted: 4 June 2013 Published: 30 July 2013
Abstract
The response patterns during water deficit stress and subsequent recovery of two forage species, Medicago truncatula and Sulla carnosa, were studied. After germination and pre-treatment, seedlings were individually cultivated for two months under two irrigation modes: 100% and 33% of field capacity. Measured parameters were plant growth, water relations, leaf osmotic potential, lipid peroxidation, and leaf inorganic (Na+ and K+) and organic (proline and soluble sugars) solute contents, as well as delta-1-pyrroline-5-carboxylate synthase (P5CS) and proline dehydrogenase (PDH) activities. Our results showed that under control conditions, and in contrast to roots, no significant differences were observed in shoot biomass production between the two species. However, when subjected to water-deficit stress, M. truncatula appeared to be more tolerant than S. carnosa (reduction by 50 and 70%, respectively). In the two studied species, water-deficit stress led to an increase in root/shoot ratio and leaf proline and soluble sugar contents, and a decrease in leaf osmotic potential. Enzymatic assay revealed that in the two species, P5CS activity was stimulated whereas that of PDH was inhibited under stress conditions. Despite greater accumulation of proline, sugar, and potassium in leaves of S. carnosa, M. truncatula was more tolerant to water deficit. This was essentially due to its capacity to control tissue hydration and water-use efficiency, in addition to its greater ability to protect membrane integrity. Following stress relief, M. truncatula and S. carnosa showed partial re-establishment of growth capacity.
Additional keywords: legume species, osmolytes, rehydration, tolerance, water relations.
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