Identification of QTLs involved in pod-shatter resistance in Brassica napus L.
Y. C. Wen A C , S. F. Zhang A , B. Yi B , J. Wen B , J. P. Wang A , J. C. Zhu A , J. P. He A and J. H. Cao A
+ Author Affiliations
- Author Affiliations
A Institute of Industrial Crops, Henan Academy of Agricultural Sciences/Henan Key Laboratory of Oilseed Crops Genetic Improvement, 450002 Zhengzhou, China.
B National Key Laboratory of Crop Genetic Improvement/National Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, 430070 Wuhan, China.
C Corresponding author. Email: yanchengwen@yahoo.com.cn
Crop and Pasture Science 63(12) 1082-1089 https://doi.org/10.1071/CP12318
Submitted: 4 September 2012 Accepted: 7 February 2013 Published: 5 March 2013
Abstract
Seed loss caused by pod-shatter during harvesting is one of the main problems in rapeseed production worldwide. Quantitative trait loci (QTLs) for pod-shatter based on genetic mapping would help breeders develop cultivars resistant to pod-shatter. In this study, we constructed a genetic map of Brassica napus containing 107 simple sequence repeat (SSR) markers and 68 sequence-related amplified polymorphism (SRAP) markers using a doubled-haploid (DH) population of 276 lines derived from the cross H155 × Qva. This map covered 1382.8 cM with an average marker interval of 7.9 cM. In total, 13 QTLs for pod-shatter resistance were identified in this DH population at two experimental sites (in Wuhan and Zhengzhou); three of the QTLs were present at both locations. At Zhengzhou, nine QTLs, identified in linkage groups A1, A7, A8, C5, and C8, together explained 49.0% of the phenotypic variation. At Wuhan, four QTLs were mapped on the A1, A4, A7, and C8 linkage groups. These QTLs explained 38.6% of the phenotypic variation. These results may serve as a valuable basis for further molecular dissection of pod-shatter resistance in B. napus, and for development of the markers related to QTLs that may be useful for marker-assisted selection of pod-shatter resistant cultivars.
Additional keywords: Brassica napus L., linkage map, molecular marker, pod-shatter resistance index, QTL.
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