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Australian Journal of Chemistry Australian Journal of Chemistry Society
An international journal for chemical science
REVIEW

Advances in high abundance protein molecular imprinting techniques in human serum

Zhipeng Liu A , Aijun Gong https://orcid.org/0000-0002-6261-1013 A B * , Lina Qiu A B , Yang Liu A , Shujia Zheng A , Wenyan Qin C and RongRong Fan D
+ Author Affiliations
- Author Affiliations

A College of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.

B Beijing Key Laboratory for Science and Application of Functional Molecular and Crystalline Materials, University of Science and Technology Beijing, Beijing, 100083, China.

C CapitalBio Technology Inc., Beijing, 101111, China.

D Kunshan Hexin Mass Spectrometry Technology Co, Ltd, Kunshan,Jiangsu, 215300, China.

* Correspondence to: Gongaijun5661@ustb.edu.cn

Handling Editor: Charlotte Williams

Australian Journal of Chemistry 76(3) 150-168 https://doi.org/10.1071/CH22223
Submitted: 18 October 2022  Accepted: 30 March 2023   Published: 8 May 2023

© 2023 The Author(s) (or their employer(s)). Published by CSIRO Publishing.

Abstract

The detection of protein biomarkers is crucial for early disease diagnosis. However, these biomarkers are present at low levels in serum, and the detection signal is easily interfered with by high levels of proteins. These factors pose major challenges for direct biomarker detection by existing technologies; thus, sample pre-treatments are performed as the best solution. Molecularly imprinted polymers have excellent properties of good binding ability, high selectivity and low cost, making this technique one of the best for serum pre-treatment. This review discusses the recent research status and development of bulk and surface imprinting techniques for high-abundance proteins. Furthermore, this paper emphasizes the research overview and progress of substrate and template selection, template immobilization technology and strategies to control the thickness of imprinted polymers when using the surface imprinting technique. Finally, the main challenges of molecular imprinting technique (MIT) application for high-abundance proteins and the future direction of this field are highlighted.

Keywords: epitope imprinting, high abundance protein, metal chelation, MIP, MIT, molecular gels, oriented immobilization, specific identification, surface imprinting.


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