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Australian Journal of Botany Australian Journal of Botany Society
Southern hemisphere botanical ecosystems
RESEARCH ARTICLE

Solving taxonomic problems within the Aldama genus based on anatomical characters

Aline Bertolosi Bombo A B C , Arinawa Liz Filartiga A B and Beatriz Appezzato-da-Glória A
+ Author Affiliations
- Author Affiliations

A Biological Sciences Department, College of Agriculture ‘Luiz de Queiroz’, University of São Paulo, 11 Pádua Dias Avenue, 13418-900, Piracicaba, SP, Brazil.

B Post-Graduate Program in Plant Biology, Institute of Biology, University of Campinas, 255 Monteiro Lobato Street, 13083-862, Campinas, SP, Brazil.

C Corresponding author. Email: aline_bbombo@hotmail.com

Australian Journal of Botany 64(6) 501-512 https://doi.org/10.1071/BT16070
Submitted: 7 April 2016  Accepted: 3 August 2016   Published: 16 September 2016

Abstract

Anatomical characteristics have proved to be an invaluable asset for supporting taxonomic studies on different plant families, such as Asteraceae. Anatomical features can also help clarify taxonomical problems in the Aldama La Llave genus, especially among Brazilian representatives. The present study focussed on Aldama bakeriana, A. discolor, A. grandiflora and A. squalida. These species were chosen because they are difficult to identify taxonomically if the specimens have no flowers, they have biological and pharmacological potential and they are representative species from key morphological groups in the Aldama genus and could help in future taxonomical investigations. Aerial and underground vegetative organs from the four species were described herein for the first time and a comparative analysis was performed to highlight the unique features of each species and determine whether these species can be differentiated in terms of anatomy. All four species analysed were anatomically very similar. However, they could be differentiated on the basis of the set of anatomical features described for each species. On the basis of our findings, we concluded that anatomy is able to provide data to assist with the taxonomic problems within the four species analysed herein. The results also corroborated other studies on the Aldama genus.

Additional keywords: aerial organs, anatomy, secretory ducts, underground system.


References

Adedeji O, Jewoola O (2008) Importance of leaf epidermal characters in the Asteraceae family. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 36, 7–16.

Ambrosio SR, Schoor K, Da Costa FB (2004) Terpenoids of Viguiera arenaria (Asteraceae). Biochemical Systematics and Ecology 32, 221–224.
Terpenoids of Viguiera arenaria (Asteraceae).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXpvVWgt7g%3D&md5=ec5877b808269c4bf6af9c32ae85b1bbCAS |

Appezzato-da-Glória B (2015) ‘Morfologia de sistemas subterrâneos de plantas. Morphology of plant underground systems.’ (3i Editora: Belo Horizonte, Brazil)

Appezzato-da-Glória B, Cury G, Soares M, Rocha R, Hayashi A (2008) Underground systems of Asteraceae species from the Brazilian cerrado. The Journal of the Torrey Botanical Society 135, 103–113.
Underground systems of Asteraceae species from the Brazilian cerrado.Crossref | GoogleScholarGoogle Scholar |

Arakawa NS, Schorr K, Ambrósio SR, Merfort I, Da Costa FB (2008) Further sesquiterpene lactones from Viguiera robusta and the potential anti-inflammatory activity of a heliangolide: inhibition of human neutrophil elastase release. Zeitschrift für Naturforschung C 63, 533–538.
Further sesquiterpene lactones from Viguiera robusta and the potential anti-inflammatory activity of a heliangolide: inhibition of human neutrophil elastase release.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhtVKnurvK&md5=a2e9ae0a0435750419444e665b8d49bcCAS |

Aschenbrenner AK, Horakh S, Spring O (2013) Linear glandular trichomes of Helianthus (Asteraceae): morphology, localization, metabolite activity and occurrence. AoB Plants 5, plt028
Linear glandular trichomes of Helianthus (Asteraceae): morphology, localization, metabolite activity and occurrence.Crossref | GoogleScholarGoogle Scholar |

Bombo AB, Oliveira TS, Oliveira ADSS, Rehder VLG, Magenta MAG, Appezzato-da-Glória B (2012) Anatomy and essential oils from aerial organs in three species of Aldama (Asteraceae–Heliantheae) that have a difficult delimitation. Australian Journal of Botany 60, 632–642.
Anatomy and essential oils from aerial organs in three species of Aldama (Asteraceae–Heliantheae) that have a difficult delimitation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhsFOlurzE&md5=bea53cc87d6042214c6899bc1354b13fCAS |

Bombo AB, Oliveira TS, Oliveira ASS, Rehder VLG, Appezzato-da-Glória B (2014) Anatomy and essential oil composition of the underground systems of three species of Aldama La Llave (Asteraceae). The Journal of the Torrey Botanical Society 141, 115–125.
Anatomy and essential oil composition of the underground systems of three species of Aldama La Llave (Asteraceae).Crossref | GoogleScholarGoogle Scholar |

Brocklebank KJ, Hendry GAE (1989) Characteristics of plant species which store different types of reserve carbohydrates. New Phytologist 112, 255–260.
Characteristics of plant species which store different types of reserve carbohydrates.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL1MXlslWhsrY%3D&md5=0906f9325f4766554de853b8bd9885ddCAS |

Bukatsch F (1972) Bermerkungen zur Doppelfarbung Astrablau–safranin. Mikrokosmos 61, 255

Carlquist S (1982) The use of ethylenediamine in softening hard plant structures for paraffin sectioning. Stain Technology 57, 311–317.
The use of ethylenediamine in softening hard plant structures for paraffin sectioning.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaL3s%2FpsVahtg%3D%3D&md5=8c33ede4e2c8f2abebd316728661a895CAS | 7179333PubMed |

Carvalho TC, Simão MR, Ambrósio SR, Furtado NA, Veneziani RC, Heleno VC, Da Costa FB, Gomes BP, Souza MG, Reis EB, Martins CH (2011) Antimicrobial activity of diterpenes from Viguiera arenaria against endodontic bacteria. Molecules (Basel, Switzerland) 16, 543–551.
Antimicrobial activity of diterpenes from Viguiera arenaria against endodontic bacteria.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhtFOhs7o%3D&md5=3571139b5350c559397d46881f83607eCAS |

Castro MDM, Leitão-Filho HDF, Monteiro WR (1997) Utilização de estruturas secretoras na identificação dos gêneros de Asteraceae de uma vegetação de cerrado. Revista Brasileira de Botanica. Brazilian Journal of Botany 20, 163–174.
Utilização de estruturas secretoras na identificação dos gêneros de Asteraceae de uma vegetação de cerrado.Crossref | GoogleScholarGoogle Scholar |

Coutinho L (1990) Fire in the ecology of the Brazilian cerrado. In ‘Fire in the tropical biota: ecosystem process and global challenges’. (Ed. J Goldamer) pp. 82–105. (Springer-Verlag: Berlin)

Cury G, Appezzato-da-Glória B (2009) Internal secretory spaces in thickened underground systems of Asteraceae species. Australian Journal of Botany 57, 229–239.
Internal secretory spaces in thickened underground systems of Asteraceae species.Crossref | GoogleScholarGoogle Scholar |

De Micco V, Aronne G (2012) Morpho-anatomical traits for plant adaptation to drought: from morphological to molecular features. In ‘Plant responses to drought stress’. (Ed. R Aroca) pp. 37–61. (Springer: Berlin)

Duarte M, Wolf S, de Paula B (2008) Smallanthus sonchifolius (Poepp.) H.Rob. (Yacon): microscopical identification of the leaf and stem for the pharmacognostic quality control. Revista Brasileira de Ciências Farmacêuticas 44, 157–164.
Smallanthus sonchifolius (Poepp.) H.Rob. (Yacon): microscopical identification of the leaf and stem for the pharmacognostic quality control.Crossref | GoogleScholarGoogle Scholar |

Fahn A (2000) Structure and function of secretory cells. Advances in Botanical Research Incorporating Advances in Plant Pathology 31, 37–75.

Fidelis A, Pivello V (2011) Deve-se esar o fogo como instrumento de manejo no cerrado e campos sulinos? Biodiversidade Brasileira 2, 12–25.

Figueiredo-Ribeiro RCL (1993) Distribulção, aspectos estruturais e funcionais dos frutanos, com ênfase em plantas herbáceas do cerrado. Revista Brasileira de Fisiologia Vegetal 5, 203–208.

Gregory M, Baas P (1989) A survey of mucilage cells in vegetative organs of the dicotyledons. Israel Journal of Botany 38, 125–174.

Hayashi A, Appezzato-da-Glória B (2007) Anatomy of the underground system in Vernonia grandiflora Less. and V. brevifolia Less. (Asteraceae). Brazilian Archives of Biology and Technology 50, 979–988.
Anatomy of the underground system in Vernonia grandiflora Less. and V. brevifolia Less. (Asteraceae).Crossref | GoogleScholarGoogle Scholar |

Horridge G, Tamm S (1969) Critical point drying for scanning electron microscopic study of ciliary motion. Science 163, 817–818.
Critical point drying for scanning electron microscopic study of ciliary motion.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3cvjsl2ktA%3D%3D&md5=ade31ef4a8e380d08a55dc29fc237af0CAS | 17807989PubMed |

Inceer H, Ozcan M (2011) Leaf anatomy as an additional taxonomy tool for 18 taxa of Matricaria L. and Tripleurospermum Sch Bip. (Anthemideae-Asteraceae) in Turkey. Plant Systematics and Evolution 296, 205–215.
Leaf anatomy as an additional taxonomy tool for 18 taxa of Matricaria L. and Tripleurospermum Sch Bip. (Anthemideae-Asteraceae) in Turkey.Crossref | GoogleScholarGoogle Scholar |

Jensen W (1962) ‘Botanical histochemistry, principles and practice.’ (W. H. Freeman: San Francisco, CA)

Johansen D (1940) ‘Plant microtechnique.’ (McGraw-Hill Book Co. Inc.: New York)

Karanović D, Luković J, Zorić L, Anačkov G, Boza P (2015) Taxonomic status of Aster, Galatella and Tripolium (Asteraceae) in view of anatomical and micro-morphological evidence. Nordic Journal of Botany 33, 484–497.
Taxonomic status of Aster, Galatella and Tripolium (Asteraceae) in view of anatomical and micro-morphological evidence.Crossref | GoogleScholarGoogle Scholar |

Karnovsky MJ (1965) A Formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. The Journal of Cell Biology 27, 137–138.

Kelsey R (1984) Glandular trichomes: a helpful taxonomic character for Artemisia nova (black sagebrush). Journal of Range Management 37, 370–372.
Glandular trichomes: a helpful taxonomic character for Artemisia nova (black sagebrush).Crossref | GoogleScholarGoogle Scholar |

Machado SR, Oliviera DMT, Dip MR, Menezes NL (2004) Morfoanatomia do sistema subterrâneo de Smallanthus sonchifolius (Poepp. & Endl.) H.Robinson (Asteraceae). Revista Brasileira de Botanica. Brazilian Journal of Botany 27, 115–123.

Magenta MAG (2006) Viguiera Kunth (Asteraceae, Heliantheae) na América do sul e sistemática das espécies do Brasil. PhD Thesis, Universidade de São Paulo, Brazil.

Magenta MAG, Pirani JR (2014) Novidades taxonômicas em Aldama (Asteraceae–Heliantheae). Rodriguésia 65, 175–192.
Novidades taxonômicas em Aldama (Asteraceae–Heliantheae).Crossref | GoogleScholarGoogle Scholar |

Magenta MAG, Pirani JR, Mondin CA (2010) Novos táxons e combinações em Viguiera (Asteraceae–Heliantheae). Rodriguésia 61, 1–11.

Marquina S, Maldonado N, Garduño-Ramírez ML, Aranda E, Villarreal ML, Navarro V, Bye R, Delgado G, Alvarez L (2001) Bioactive oleanolic acid saponins and other constituents from the roots of Viguiera decurrens. Pytochemistry 56, 93–97.
Bioactive oleanolic acid saponins and other constituents from the roots of Viguiera decurrens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXitlaruw%3D%3D&md5=ed1c07d9c54ceea53d80ea2bdbe1f0f6CAS |

McManus J (1948) Histological and histochemical uses of periodic acid. Stain Technology 23, 99–108.
Histological and histochemical uses of periodic acid.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaH1cXjt1amsQ%3D%3D&md5=33aa2602e7c6f18d3d5eb421d41f9c4eCAS | 18867618PubMed |

Metcalfe CR, Chalk L (1950) ‘Anatomy of the dicotyledons.’ (Clarendon Press: Oxford, UK)

Nicolete R, Arakawa NS, Rius C, Nomizo A, Jose PJ, Da Costa FB, Sanz MJ, Faccioli LH (2009) Budlein A from Viguiera robusta inhibits leukocyte-endothelial cell interactions, adhesion molecule expression and inflammatory mediators release. Phytomedicine 16, 904–915.
Budlein A from Viguiera robusta inhibits leukocyte-endothelial cell interactions, adhesion molecule expression and inflammatory mediators release.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhtlKqt73E&md5=0990743c268703be8fd033260ca25752CAS | 19524419PubMed |

Oliveira TSd, Bombo AB, Appezzato-da-Glória B (2013) Anatomy of vegetative organs with an emphasis on the secretory structures of two species of Aldama (Asteraceae–Heliantheae). Botany 91, 335–342.
Anatomy of vegetative organs with an emphasis on the secretory structures of two species of Aldama (Asteraceae–Heliantheae).Crossref | GoogleScholarGoogle Scholar |

Paviani T (1987) Anatomia do desenvolvimento do xilopódio de Brasilia sickii G.M. Barroso. Estágio inicial. Ciencia e Cultura 39, 399–405.

Porto TS, Furtado NAJC, Heleno VCG, Martins CHG, Da Costa FB, Severiano ME, Silva AN, Veneziani RCS, Ambrósio SR (2009) Antimicrobial ent-pimarane diterpenes from Viguiera arenaria against Gram-positive bactéria. Fitoterapia 80, 432–436.
Antimicrobial ent-pimarane diterpenes from Viguiera arenaria against Gram-positive bactéria.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhtVKmu73E&md5=eeba34322ae7702e4b0b54d9ca066958CAS | 19524643PubMed |

Ramayya N (1962) Studies on the trichomes of some Compositae II. Phylogeny and classification. Bulletin of the Botanical Survey of India 4, 189–192.

Rizzini C (1965) Estudos experimentais sobre o xilopódio e outros órgãos tuberosos em plantas do cerrado. Anais da Academia Brasileira de Ciencias 37, 87–113.

Rizzini C, Heringer E (1961) Underground organs of plants from some southern Brazilian savannas, with special reference to the xylopodium. Phyton 17, 105–124.

Robinson H, Moore A (2004) New species and new combinations in Rhysolepis (Heliantheae: Asteraceae). Proceedings of the Biological Society of Washington 117, 423–446.

Sakai W (1973) Simple method for differential staining of paraffin embedded plant material using toluidine blue O. Stain Technology 48, 247–249.
Simple method for differential staining of paraffin embedded plant material using toluidine blue O.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE3sXlt1ehu70%3D&md5=67b2ef1f73c73d09305ddcead8f955b6CAS | 4126691PubMed |

Scatena V, Giulietti A, Borba E, Van den Berg C (2005) Anatomy of Brazilian Eriocaulaceae: correlation with taxonomy and habitat using multivariate analyses. Plant Systematics and Evolution 253, 1–22.
Anatomy of Brazilian Eriocaulaceae: correlation with taxonomy and habitat using multivariate analyses.Crossref | GoogleScholarGoogle Scholar |

Schilling E, Panero J (1991) Evidence for a close relationship between Iostephane and Viguiera (Asteraceae, Heliantheae). American Journal of Botany 78, 1054–1062.
Evidence for a close relationship between Iostephane and Viguiera (Asteraceae, Heliantheae).Crossref | GoogleScholarGoogle Scholar |

Schilling E, Panero J (1996) Phylogenetic reticulation in subtribe Helianthinae. American Journal of Botany 83, 939–948.
Phylogenetic reticulation in subtribe Helianthinae.Crossref | GoogleScholarGoogle Scholar |

Schilling E, Panero J (2011) A revised classification of subtribe Helianthinae (Asteraceae: Heliantheae) II. Derived lineages. Botanical Journal of the Linnean Society 167, 311–331.
A revised classification of subtribe Helianthinae (Asteraceae: Heliantheae) II. Derived lineages.Crossref | GoogleScholarGoogle Scholar |

Schilling EE, Da Costa FB, Lopes NP, Heise PJ (2000) Brazilian species of Viguiera (Asteraceae) exhibit low levels of its sequence variation. Edinburgh Journal of Botany 57, 323–332.
Brazilian species of Viguiera (Asteraceae) exhibit low levels of its sequence variation.Crossref | GoogleScholarGoogle Scholar |

Silva EMS, Hayashi AH, Appezzato-da-Glória B (2014) Anatomy of vegetative organs in Aldama tenuifolia and A. kunthiana (Asteraceae: Heliantheae). Brazilian Journal of Botany 37, 505–517.
Anatomy of vegetative organs in Aldama tenuifolia and A. kunthiana (Asteraceae: Heliantheae).Crossref | GoogleScholarGoogle Scholar |

Solereder H (1908) ‘Systematic anatomy of the dicotyledons: a handbook for laboratories of pure and applied botany.’ (Clarendon Press: Oxford, UK)

Sosa MdlM, Pico GMVd, Dematteis M (2014) Comparative anatomy of leaves and stems in some species of the South American genus Chrysolaena (Vernonieae, Asteraceae) and taxonomic implications. Nordic Journal of Botany 32, 611–619.
Comparative anatomy of leaves and stems in some species of the South American genus Chrysolaena (Vernonieae, Asteraceae) and taxonomic implications.Crossref | GoogleScholarGoogle Scholar |

Spring O, Rodon U, Macias F (1992) Sesquiterpenes from noncapitate glandular trichomes of Helianthus annuus. Phytochemistry 31, 1541–1544.
Sesquiterpenes from noncapitate glandular trichomes of Helianthus annuus.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK38XksVCmu7w%3D&md5=1d4959e89aa07467a55372c9940716bcCAS |

Strasburger E (1913) ‘Handbook of practical botany.’ 7th edn. (George Allen: London)

Thadeo M, Azevedo A, Meira R (2014) Foliar anatomy of neotropical Salicaceae: potentially useful characters for taxonomy. Plant Systematics and Evolution 300, 2073–2089.
Foliar anatomy of neotropical Salicaceae: potentially useful characters for taxonomy.Crossref | GoogleScholarGoogle Scholar |

Valluru R, Van den Ende W (2008) Plant fructans in stress environments: emerging concepts and future prospects. Journal of Experimental Botany 59, 2905–2916.
Plant fructans in stress environments: emerging concepts and future prospects.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXpslalsL4%3D&md5=e2b5f7bd75c79f22211a77dda7a16821CAS | 18603617PubMed |

Vilhalva DAA, Appezzato-da-Glória B (2006) Morfoanatomia da raiz tuberosa de Vernonia oxylepis Sch. Bip. in Mart. ex Baker – Asteraceae. Acta Botanica Brasílica 20, 591–598.
Morfoanatomia da raiz tuberosa de Vernonia oxylepis Sch. Bip. in Mart. ex Baker – Asteraceae.Crossref | GoogleScholarGoogle Scholar |

Vilhalva D, Cortelazzo A, Carvalho A, Figueiredo-Ribeiro R (2011) Histochemistry and ultrastructure of Campuloclinium chlorolepis (Asteraceae) tuberous roots accumulating fructan: evidences of functions other than reserve carbohydrate. Australian Journal of Botany 59, 46–52.
Histochemistry and ultrastructure of Campuloclinium chlorolepis (Asteraceae) tuberous roots accumulating fructan: evidences of functions other than reserve carbohydrate.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhslGisL0%3D&md5=2102477b3c71de013f13e2d7d672762bCAS |

Warming E (1908) ‘Lagoa Santa. Contribuição para a geographia phytobiologica.’ (Imprensa Oficial do Estado de Minas Gerais: Belo Horizonte)