Histochemistry and ultrastructure of Campuloclinium chlorolepis (Asteraceae) tuberous roots accumulating fructan: evidences of functions other than reserve carbohydrate
Divina A. A. Vilhalva A , Angelo L. Cortelazzo A , A. L. Maria Angela M. Carvalho B and Rita de Cássia L. Figueiredo-Ribeiro B C
+ Author Affiliations
- Author Affiliations
A Departamento de Biologia Celular e Estrutural, Universidade Estadual de Campinas, Av. Bertrand Roussel s/n, Cep 13083-865, Campinas, SP, Brazil.
B Núcleo de Pesquisa em Fisiologia e Bioquímica, Instituto de Botânica, Caixa Postal 3005, Cep 01061-970, São Paulo, SP, Brazil.
C Corresponding author. Email: rita.cassia@pq.cnpq.br
Australian Journal of Botany 59(1) 46-52 https://doi.org/10.1071/BT10126
Submitted: 22 May 2010 Accepted: 14 December 2010 Published: 10 February 2011
Abstract
Campuloclinium chlorolepis (Baker), an herbaceous species of the Cerrado, accumulates inulin-type fructans in the tuberous roots. Plants were collected in the Cerrado and the roots analysed using light and scanning electron microscopy. The presence of fructans was assessed by specific stain reactions and by high performance anion exchange chromatography. Here, we report the localisation of protein, phenols and neutral polysaccharides in the tuberous roots after staining with different dyes generally used in cytochemical analyses. Results showed the presence of fructans inside and outside cells from all tissues of tuberous roots with the exception of the epidermis. When visualised by scanning electron microscopy, globular bodies consistent with typical inulin spherocrystals were clearly detected under polarised light. These globular bodies varied in size according to location, being smaller in the cortical tissue and larger in the central cylinder. The localisation of fructans outside the cell in several tissues of the tuberous roots clearly shown by histochemical and ultrastructural analyses lead to the hypothesis of interaction of fructose polymers with cell membrane and possibly their role in membrane stabilisation in plants subjected to stressing environmental conditions.
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