Nuclear DNA content of three Eucalyptus species estimated by flow and image cytometry
Milene Miranda Praça A , Carlos Roberto Carvalho A C and Carolina Ribeiro Diniz Boaventura Novaes BA Laboratório de Citogenética e Citometria, Departamento de Biologia Geral, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil.
B School of Forest Resources and Conservation, University of Florida, Gainesville, Florida, 32611 USA.
C Corresponding author. Email: ccarvalh@ufv.br
Australian Journal of Botany 57(6) 524-531 https://doi.org/10.1071/BT09114
Submitted: 27 June 2009 Accepted: 15 September 2009 Published: 9 November 2009
Abstract
Previous flow cytometry (FCM) analyses delivered nearly equal mean values of nuclear 2C DNA content for Eucalyptus grandis Hill ex Maiden and E. urophylla S. T. Blake (1.33 pg and 1.34 pg, respectively), whereas E. globulus Labill. presented distinct mean values (1.09, 1.13 and 1.40). These differences have been attributed to the different methodological approach, utilised plant cultivar and presence of intrinsic metabolic compounds that affect fluorochrome fluorescence. In the present study, a FCM and image cytometry (ICM) design, following international consensus criteria, were adopted to reassess the nuclear DNA contents of the above-mentioned Eucalyptus species. Statistical analyses revealed either similar or discrepant nuclear DNA contents, depending on the standard species used and whether FCM or ICM was employed. Our results demonstrated that 2C DNA values obtained by FCM and ICM were most uniform when Solanum lycopersicum was used as a standard. Moreover, the values obtained for E. grandis and E. urophylla were close, but differed as much as 24.63% in relation to previous data, and E. globulus proportionally varied up to 25%. New DNA content values are suggested for these eucalypt species.
Acknowledgements
We thank CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil) and FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais) for providing the financial support for this work.
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