Pollen treatment for mutation induction in Eucalyptus globulus ssp. globulus (Myrtaceae)
L. J. McManus A , J. Sasse B , C. K. Blomstedt A C and G. Bossinger A DA The School of Forest and Ecosystem Science, University of Melbourne, Creswick, Vic. 3363, Australia.
B Sassafras Group, 2 O’Farrell Street, Yarraville, Vic. 3013, Australia.
C Current address: The School of Biological Sciences, Monash University, Clayton, Vic. 3800, Australia.
D Corresponding author. Email: gerd@unimelb.edu.au
Australian Journal of Botany 54(1) 65-71 https://doi.org/10.1071/BT05094
Submitted: 30 May 2005 Accepted: 8 September 2005 Published: 22 February 2006
Abstract
Mutation induction has played an integral role in the improvement of most commercially important crop species but has not been successfully applied to tree species because of their long reproductive cycles which hinder the use of the traditional seed-treatment approaches. Treatment of pollen with a chemical mutagen prior to pollination will, theoretically, allow stable, heterozygous mutant trees to be produced in a relatively short time and might facilitate mutagenesis of tree species. As the first step in testing this hypothesis, a controlled-pollination trial with chemically treated pollen was conducted in Eucalyptus globulus ssp. globulus (Labill.). Assessment of fruit, seed and seedlings from more than 500 pollinations associated mutagenic treatment of pollen with a significant reduction in seed set. Non-significant increases in capsule (fruit) abortion, the inhibition of seed germination and the incidence of aberration in seedlings were also noted. We argue that pollen treatment may be a useful means of producing Eucalyptus mutants with variation in flowering time, salinity and frost tolerance, lignification and other traits of scientific and economic importance.
Acknowledgments
We thank L. Hodge, P. Buxton of Grand Ridge Plantations and S. Elms of Hancock Victorian Plantations for their assistance in establishment and monitoring of the trial.
Ahloowalia BS,
Maluszynski M, Nichterlein K
(2004) Global impact of mutation-derived varieties. Euphytica 135, 187–204.
| Crossref | GoogleScholarGoogle Scholar |
Chaffey N,
Cholewa E,
Regan S, Sundberg B
(2002) Secondary xylem development in Arabidopsis: a model for wood formation. Physiologia Plantarum 114, 594–600.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Charlesworth B, Charlesworth D
(1998) Some evolutionary consequences of deleterious mutations. Genetica 102–103, 3–19.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Colbert T,
Till B,
Tompa R,
Reynolds S,
Steine M,
Yeung A,
McCallum C,
Comai L, Henikoff S
(2001) High-throughput screening for induced point mutations. Plant Physiology 126, 480–484.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Dimmel DR,
MacKay JJ,
Courchene CE,
Kadla JF,
Scott JT,
O’Malley DM, McKeand SE
(2002) Pulping and bleaching of partially CAD-deficient wood. Journal of Wood Chemistry and Technology 22, 235–248.
| Crossref | GoogleScholarGoogle Scholar |
Goujon T,
Sibout R,
Pollet B,
Maba B,
Nussaume L,
Bechtold N,
Lu FC,
Ralph J,
Mila I,
Barriere Y,
Lapierre C, Jouanin L
(2003) A new Arabidopsis thaliana mutant deficient in the expression of O-methyltransferase impacts lignins and sinapoyl esters. Plant Molecular Biology 51, 973–989.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Greene EA,
Codomo CA,
Taylor NE,
Henikoff JG,
Till BJ,
Reynolds SH,
Enns LC,
Burtner C,
Johnson JE,
Odden AR,
Comai L, Henikoff S
(2003) Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis. Genetics 164, 731–740.
| PubMed |
Griffin AR,
Ching KK,
Johnson KW,
Hand FC, Burgess IP
(1982) Processing Eucalyptus regnans pollen for use in controlled pollination. Silvae Genetica 31, 198–203.
Halpin C,
Holt K,
Chojecki J,
Oliver D,
Chabbert B,
Monties B,
Edwards K,
Barakate A, Foxon GA
(1998) Brown-midrib maize (bm1)—a mutation affecting the cinnamyl alcohol dehydrogenase gene. The Plant Journal 14, 545–553.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Harbard JL,
Griffin AR, Espejo J
(1999) Mass controlled pollination of Eucalyptus globulus: a practical reality. Canadian Journal of Forest Research 29, 1457–1463.
| Crossref | GoogleScholarGoogle Scholar |
Henikoff S, Comai L
(2003) Single nucleotide mutations for plant functional genomics. Annual Review of Plant Biology 54, 375–401.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Mackay JJ,
O’Malley DM,
Presnell T,
Booker FL,
Campbell MM,
Whetten RW, Sederoff RR
(1997) Inheritance, gene expression, and lignin characterization in a mutant pine deficient in cinnamyl alcohol dehydrogenase. Proceedings of the National Academy of Sciences, USA 94, 8255–8260.
| Crossref | GoogleScholarGoogle Scholar |
Marita JM,
Ralph J,
Hatfield RD, Chapple C
(1999) NMR characterization of lignins in Arabidopsis altered in the activity of ferulate 5-hydroxylase. Proceedings of the National Academy of Sciences, USA 96, 12 328–12 332.
| Crossref | GoogleScholarGoogle Scholar |
Marita JM,
Vermerris W,
Ralph J, Hatfield RD
(2003) Variations in the cell wall composition of maize brown midrib mutants. Journal of Agricultural and Food Chemistry 51, 1313–1321.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Meyer K,
Cusumano JC,
Somerville C, Chapple CCS
(1996) Ferulate-5-hydroxylase from Arabidopsis thaliana defines a new family of cytochrome P450-dependent monooxygenases. Proceedings of the National Academy of Sciences, USA 93, 6869–6874.
| Crossref | GoogleScholarGoogle Scholar |
Neuffer MG
(1971) Chemical mutagens and in vitro germination of pollen. Maize Genetics Cooperation News Letter 45, 145–146.
Neuffer MG, Coe EH
(1978) Paraffin oil technique for treating mature corn pollen with chemical mutagens. Maydica 23, 21–28.
Neuffer, MG ,
Coe, EH ,
and
Wessler, SR (1990). Mutagenesis. In ‘Mutants of maize’. pp. 395–401. (Cold Springs Harbor Laboratory Press: New York)
Oakeley EJ,
Podesta A, Jost JP
(1997) Developmental changes in DNA methylation of the two tobacco pollen nuclei during maturation. Proceedings of the National Academy of Sciences, USA 94, 11 721–11 725.
| Crossref | GoogleScholarGoogle Scholar |
Poke FS,
Vaillancourt RE,
Elliott RC, Reid JB
(2003) Sequence variation in two lignin biosynthesis genes, cinnamoyl CoA reductase (CCR) and cinnamyl alcohol dehydrogenase 2 (CAD2). Molecular Breeding 12, 107–118.
| Crossref | GoogleScholarGoogle Scholar |
Pound LM,
Wallwork MAB,
Potts BM, Sedgley M
(2002) Self-incompatibility in Eucalyptus globulus ssp globulus (Myrtaceae). Australian Journal of Botany 50, 365–372.
| Crossref | GoogleScholarGoogle Scholar |
Pound LM,
Patterson B,
Wallwork MAB,
Potts BM, Sedgley M
(2003) Pollen competition does not affect the success of self-pollination in Eucalyptus globulus (Myrtaceae). Australian Journal of Botany 51, 189–195.
| Crossref | GoogleScholarGoogle Scholar |
Ralph J,
Mackay JJ,
Hatfield RD,
O’Malley DM,
Whetten RW, Sederoff RR
(1997) Abnormal lignin in a loblolly pine mutant. Science 277, 235–239..
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Twell D
(1992) Use of a nuclear-targeted β-glucuronidase fusions protein to demonstrate vegetative cell-specific gene expression in developing pollen. The Plant Journal 2, 887–892.
| Crossref | GoogleScholarGoogle Scholar |
