The assessment of TGGE for the detection of interspecific and intergeneric DNA-marker polymorphism within Solanaceae
S. H. GarlandA Department of Primary Industries and Fisheries, GPO Box 46, Brisbane, Qld 4001, Australia.
B Email: Stephen.Garland@dpi.qld.gov.au
Australian Journal of Agricultural Research 56(3) 291-299 https://doi.org/10.1071/AR04141
Submitted: 21 June 2004 Accepted: 27 January 2005 Published: 23 March 2005
Abstract
RFLP markers are currently the most appropriate marker system for the identification of uncharacterised polymorphism at the interspecific and intergeneric level. Given the benefits of a PCR-based marker system and the availability of sequence information for many Solanaceous cDNA clones, it is now possible to target conserved fragments, for primer development, that flank sequences possessing interspecific polymorphism. The potential outcome is the development of a suite of markers that amplify widely in Solanaceae. Temperature gradient gel electrophoresis (TGGE) is a relatively inexpensive gel-based system that is suitable for the detection of most single-base changes. TGGE can be used to screen for both known and unknown polymorphisms, and has been assessed here, for the development of PCR-based markers that are useful for the detection of interspecific variation within Solanaceae. Fifteen markers are presented where differences between Lycopersicon esculentum and L. pennellii have been detected by TGGE. The markers were assessed on a wider selection of plant species and found to be potentially useful for the identification of interspecific and intergeneric polymorphism in Solanaceous plants.
Additional keywords: tomato, potato, Solanum, capsicum, pepper.
Acknowledgments
This research was funded by the Queensland Government’s Department of Primary Industries and Fisheries. The F2 mapping population was kindly supplied by Des McGrath and Ian Walker, Queensland Government’s Department of Primary Industries and Fisheries. The introgression lines were kindly supplied by the Tomato Genetics Resource Center, University of California, Davis. Thank you to Dr Mandy Christopher and Dr Wendy Lawson of the Queensland Government’s Department of Primary Industries and Fisheries, for their helpful review of the manuscript.
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