The development of an improved PCR-based marker system for Sw-5, an important TSWV resistance gene of tomato
S. Garland A B , M. Sharman A , D. Persley A and D. McGrath AA Department of Primary Industries and Fisheries, GPO Box 46, Brisbane, Qld 4001, Australia.
B Corresponding author. Email: Stephen.Garland@dpi.qld.gov.au
Australian Journal of Agricultural Research 56(3) 285-289 https://doi.org/10.1071/AR04140
Submitted: 21 June 2004 Accepted: 27 January 2005 Published: 23 March 2005
Abstract
Sw-5 is an important disease resistance gene of tomato, providing broad resistance to Tomato spotted wilt virus (TSWV). A cleaved amplified polymorphic sequence (CAPS) marker, closely linked to the gene, has been reported. Although the Sw-5 locus has been characterised, a gene-specific marker has not been developed. This paper presents a PCR-based marker-system that consists of the co-amplification of a dominant marker representing the Sw-5 gene sequence, and the modified CAPS marker as a positive control and indicator of genotype.
Additional keywords: rapid DNA extraction.
Acknowledgments
This research was jointly funded by the Queensland Government’s Department of Primary Industries and Fisheries (DPI&F), Australia; Horticulture Australia Limited (Project No. VG97025, Queensland Fresh Market Tomato Breeding); and Queensland Fruit and Vegetable Growers Limited (QFVG). Thank you to Dr John Thomas and Dr Mike Smith, of DPI&F, for kindly reviewing the manuscript.
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